【摘要】：甲胎蛋白(AFP)作為腫瘤標(biāo)志物,其檢測在肝癌早期診斷過程中有著重要意義。磁分離技術(shù)能夠?qū)崿F(xiàn)快速無損分離,可以有效簡化實驗過程。本實驗用免疫磁珠作為固相載體連接捕獲抗體,并與酶標(biāo)二抗通過AFP連接形成夾心結(jié)構(gòu),酶催化外加底物使其顯色,最終用紫外分光光度法檢測底物濃度間接實現(xiàn)對溶液中AFP的定量檢測。體系對AFP的檢測范圍為10~80ng·mL-1,檢出限為3.6ng·mL-1。該方法靈敏度高,重現(xiàn)性好,為AFP的檢測提供了新的思路和方法。
[Abstract]:As a tumor marker, the detection of alpha-fetoprotein (AFP) plays an important role in the early diagnosis of liver cancer. Magnetic separation technology can realize fast and lossless separation, and simplify the experimental process effectively. In this experiment, immunomagnetic beads were used as solid phase carrier to capture antibody, and the antibody was linked with enzyme labeled second antibody through AFP to form a sandwich structure, and enzyme catalyzed the addition of substrate to make it color. Finally, the quantitative detection of AFP in solution was realized indirectly by UV spectrophotometry. The detection range of AFP is that the detection limit of 10~80ng mL-1, is 3.6ng mL-1.. This method has high sensitivity and good reproducibility, which provides a new method for AFP detection.
【作者單位】： 北京師范大學(xué)分析測試中心;
【分類號】：R730.43;O657.3

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