【摘要】：目的:建立RP-HPLC法同時測定復(fù)方消銀顆粒中沒食子酸、黃芩苷和黃芩素3個化學(xué)成分含量。方法:采用Shim-pack ODS(250 mm×4.6 mm,5μm)色譜柱,以乙腈(A)-0.1%磷酸水溶液(B)為流動相,梯度洗脫(0~22 min,3%A→10%A;22~35 min,10%A→35%A;35~50 min,35%A→28%A),流速1.0 m L·min~(-1),檢測波長為280 nm,柱溫35℃。結(jié)果:沒食子酸、黃芩苷和黃芩素質(zhì)量濃度分別在3.57~49.98μg·m L~(-1)(r=0.999 5)、4.032~40.32μg·m L~(-1)(r=0.999 4)和3.278~16.390μg·m L~(-1)(r=0.999 3)范圍內(nèi)呈良好線性關(guān)系,平均回收率在98.74%~100.1%范圍內(nèi),RSD在0.49%~1.5%范圍內(nèi);3批樣品中沒食子酸、黃芩苷和黃芩素含量分別為0.153~0.154、0.256~0.258、0.113~0.114 mg·g~(-1)。結(jié)論:本方法可為復(fù)方消銀顆粒多指標(biāo)質(zhì)量評價及控制提供科學(xué)依據(jù)。
[Abstract]:Objective: to establish a RP-HPLC method for the simultaneous determination of Gallic acid, baicalin and baicalin in compound Xiaoyin granules. Methods: Shim-pack ODS (250 mm 脳 4. 6 mm,5 渭 m) column was used with acetonitrile (A)-0.1% phosphoric acid solution (B) as mobile phase. Gradient elution was used. The flow rate was 1.0 mL min~ (-1). The detection wavelength was 280 nm, column temperature 35 鈩，

本文編號：2316925