【摘要】：浙貝母(Fritillariae thunbergii Bulbus)是一種常用的化痰止咳中藥,為浙江著名的"浙八味"之一。硫熏能夠使浙貝母增白、防蟲蛀以及延長保質(zhì)期,然而過度的硫熏不僅會影響浙貝母的品質(zhì),還會危害人體健康。因此,進(jìn)行硫熏浙貝母的無損鑒別分析有利于浙貝母的品質(zhì)監(jiān)測,保障中藥質(zhì)量。采用近紅外光譜結(jié)合化學(xué)計(jì)量學(xué)方法進(jìn)行六種不同硫熏程度浙貝母的鑒別分析,在近紅外(900~1 700nm)光譜條件下,采用"boxplot"統(tǒng)計(jì)分析1 000~1 100nm內(nèi)樣本間的光譜反射值的差異。同時(shí)采用主成分分析(PCA)進(jìn)行六種樣本的聚類分析。應(yīng)用連續(xù)投影法(SPA)進(jìn)行數(shù)據(jù)挖掘獲得10條特征波段,建立其偏最小二乘判別分析(PLS-DA)模型。結(jié)果表明,建立的PLS-DA模型可達(dá)到與全譜類似的判別結(jié)果。近紅外光譜技術(shù)結(jié)合化學(xué)計(jì)量學(xué)方法能夠?qū)崿F(xiàn)不同硫熏程度浙貝母的無損鑒別分析,這為后續(xù)進(jìn)行硫熏浙貝母品質(zhì)分析以及研發(fā)相應(yīng)貝母便攜檢測儀提供參考。
[Abstract]:Zhejiang Fritillaria (Fritillariae thunbergii Bulbus) is a common Chinese medicine for eliminating phlegm and relieving cough, and it is one of the famous "eight flavors of Zhejiang" in Zhejiang Province. Sulfur fumigation can whiten Fritillaria thunbergii, prevent insects and prolong shelf life. However, excessive sulfur fumigation will not only affect the quality of Fritillaria thunbergii, but also endanger human health. Therefore, nondestructive identification analysis is beneficial to the quality monitoring of Fritillaria thunbergii and ensuring the quality of traditional Chinese medicine. Six different degree of sulfur fumigation of Fritillaria thunbergii were identified by means of near infrared spectroscopy (NIR) combined with chemometrics. Under the condition of near infrared spectrum (900 ~ 1 700nm), the difference of spectral reflectance between samples in 1 000 ~ 1 100nm was analyzed by "boxplot" statistics. At the same time, principal component analysis (PCA) was used to cluster the six samples. The continuous projection method (SPA) is used for data mining to obtain 10 feature bands, and its partial least squares discriminant analysis (PLS-DA) model is established. The results show that the established PLS-DA model can achieve a discriminant result similar to that of the whole spectrum. Near-infrared spectroscopy (NIR) combined with chemometrics can achieve nondestructive identification of Fritillaria fritillariae with different degrees of sulfur fumigation, which provides a reference for the further analysis of the quality of Fritillaria chinensis and the development of a portable detector for Fritillaria chinensis.
【作者單位】： 浙江省中醫(yī)藥研究院浙江省中藥新藥研發(fā)重點(diǎn)實(shí)驗(yàn)室;浙江大學(xué)生物系統(tǒng)工程與食品科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(31471417) 浙江省科技計(jì)劃項(xiàng)目(2015C37060,2015F50018)資助
【分類號】：O657.33;R284.1
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本文編號：2307913