【摘要】：為探索大豆異黃酮乙醇提取液的高效液相色譜檢測(cè)方法,本研究按照改進(jìn)后的色譜條件對(duì)分析方法學(xué)進(jìn)行了評(píng)價(jià)。確定的色譜條件如下,流動(dòng)相為0.1%乙酸-水溶液和0.1%乙酸-乙腈溶液,梯度洗脫;柱溫30℃;檢測(cè)波長(zhǎng)260 nm;流速1.0 m L/min。六種大豆異黃酮線性范圍良好,決定系數(shù)R20.9993,回收率范圍99.62%~114.00%,相對(duì)標(biāo)準(zhǔn)偏差RSD范圍0.96%~7.99%。本試驗(yàn)改進(jìn)的檢測(cè)方法線性關(guān)系、精密度、穩(wěn)定性、準(zhǔn)確度、靈敏度均較好,滿足分析方法學(xué)評(píng)價(jià)的要求及大豆中六種異黃酮單體檢測(cè)的要求,為改進(jìn)大豆中異黃酮的檢測(cè)方法提供了參考。
[Abstract]:In order to explore the determination method of soybean isoflavone ethanol extract by high performance liquid chromatography (HPLC), the analytical methodology was evaluated according to the improved chromatographic conditions. The chromatographic conditions are as follows: mobile phase: 0.1% acetic acid-aqueous solution and 0.1% acetic acid-acetonitrile solution, gradient elution, column temperature 30 鈩，

本文編號(hào)：2291500