二氧化鈦-AgNPs表面增強拉曼基底活性的研究
發(fā)布時間：2018-10-22 08:41
【摘要】：拉曼散射效應信號強度弱是拉曼散射本身固有的特性,因而導致檢測靈敏度相應較低,在微量和痕量分析時發(fā)生困難。表面增強拉曼光譜技術能有效地克服這個弱點,使拉曼光譜技術的應用擴大到更為廣泛的領域。尤其在生物醫(yī)學領域,在過去的20年以來,拉曼光譜技術顯示了很寬闊的應用前景,為醫(yī)學工作者在解釋疾病進展和病原學,以及在改善臨床診斷和治療方面都能提供很有價值的信息。本文除對拉曼光譜技術的基礎知識進行相關介紹外,著重介紹了二氧化鈦-AgNPs表面增強拉曼基底的制備及其活性的研究,采用532nm、632.8nm和785nm三種不同波長激光束,檢測羅丹明6g(R6g)和健康人血清兩種樣品的表面增強拉曼光譜(SERSp),并驗證不同波長激光激勵對SERSp指紋譜的影響。結果表明：當提拉速度達到200mm/min時,二氧化鈦薄膜光催化活性不再增加,影響基底活性的主要因素為粒子間距；440度-600度范圍內,二氧化鈦晶型均為銳鈦礦結構；膜厚、溫度及紫外燈照射時間共同影響銀粒子生長,200mm/min提拉速度、520℃煅燒溫度及紫外光照射80 min條件下獲得的二氧化鈦-AgNPs基底具有較好的拉曼增強效果；不同波長激勵對SERSp各譜線的增強有明顯的影響,在532nm波長激勵的SERSp中,大波數的譜峰增強因子明顯高于小波數的譜峰,在785nm波長激勵的SERSp中又正好相反,而在632.8nm波長激勵的SERSp中,大、小波數譜峰增強因子比較均一。不同波長激勵樣品SERSp指紋譜有明顯的差異。最后介紹了該基底在肺癌篩查中的應用,初步結果表明,利用532nm波長激發(fā)光及支持向量機的分類方法,肺癌篩查正確率達89%,說明該方法具有進一步研究及實用的價值。
[Abstract]:The weak intensity of Raman scattering signal is the inherent characteristic of Raman scattering, which leads to low detection sensitivity and difficulty in trace and trace analysis. Surface-enhanced Raman spectroscopy can effectively overcome this weakness and expand the application of Raman spectroscopy to a wider range of fields. Especially in the field of biomedicine, over the past 20 years, Raman spectroscopy has shown a broad application prospect for medical workers in explaining disease progress and etiology, And can provide valuable information in improving clinical diagnosis and treatment. In addition to the introduction of the basic knowledge of Raman spectroscopy, the preparation and activity of TIO _ 2 AgNPs surface-enhanced Raman substrate are introduced in this paper. The laser beams of 532nm and 632.8 nm and 785nm are used. The surface enhanced Raman spectra (SERSp),) of Rhodamine 6g (R6g) and healthy serum samples were determined and the effects of laser excitation at different wavelengths on SERSp fingerprints were verified. The results show that the photocatalytic activity of TIO _ 2 films does not increase when the Czochralski rate reaches 200mm/min, and the main factors affecting the substrate activity are the particle spacing, the crystalline form of TIO _ 2 is anatase structure and the film thickness is in the range of 440 ~ 600 degrees. The growth of silver particles was affected by temperature and irradiation time of UV lamp. The 200mm/min Czochralski rate, calcination temperature at 520 鈩，
本文編號：2286671
[Abstract]:The weak intensity of Raman scattering signal is the inherent characteristic of Raman scattering, which leads to low detection sensitivity and difficulty in trace and trace analysis. Surface-enhanced Raman spectroscopy can effectively overcome this weakness and expand the application of Raman spectroscopy to a wider range of fields. Especially in the field of biomedicine, over the past 20 years, Raman spectroscopy has shown a broad application prospect for medical workers in explaining disease progress and etiology, And can provide valuable information in improving clinical diagnosis and treatment. In addition to the introduction of the basic knowledge of Raman spectroscopy, the preparation and activity of TIO _ 2 AgNPs surface-enhanced Raman substrate are introduced in this paper. The laser beams of 532nm and 632.8 nm and 785nm are used. The surface enhanced Raman spectra (SERSp),) of Rhodamine 6g (R6g) and healthy serum samples were determined and the effects of laser excitation at different wavelengths on SERSp fingerprints were verified. The results show that the photocatalytic activity of TIO _ 2 films does not increase when the Czochralski rate reaches 200mm/min, and the main factors affecting the substrate activity are the particle spacing, the crystalline form of TIO _ 2 is anatase structure and the film thickness is in the range of 440 ~ 600 degrees. The growth of silver particles was affected by temperature and irradiation time of UV lamp. The 200mm/min Czochralski rate, calcination temperature at 520 鈩，
本文編號：2286671
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