【摘要】：采用213 nm-納秒激光剝蝕系統(tǒng)對生物基體樣品的剝蝕顆粒進(jìn)行研究,優(yōu)化了激光剝蝕條件。在剝蝕能量為25%,束斑直徑為200μm,剝蝕速率為20μm/s,頻率為20 Hz,載氣為700 m L He+700 m L Ar時,信號強(qiáng)度及穩(wěn)定性最佳。以~(31)P為內(nèi)標(biāo)元素,最佳剝蝕條件下,考察了56個元素的相對分餾因子。結(jié)果表明,生物基體的剝蝕顆粒相較于NIST 610玻璃標(biāo)樣更大,達(dá)到3μm;生物基體中元素分餾效應(yīng)相較于玻璃基體小,大多數(shù)元素的相對分餾因子達(dá)到1.0±0.1。探討了生物基體中元素分餾機(jī)理,分析了生物基體相較于玻璃基體剝蝕顆粒大,而相對分餾因子未明顯增大的原因。一方面可能是粒徑3μm的顆粒進(jìn)入電感耦合等離子體后能原子化;另一方面,大的剝蝕顆粒的富集效應(yīng)相對較小。進(jìn)一步對分餾效應(yīng)的影響因素進(jìn)行研究,發(fā)現(xiàn)分餾效應(yīng)與激光剝蝕能量、激光頻率和掃描速率相關(guān),并且與元素的氧化物沸點負(fù)相關(guān),與氧化物鍵能和電離能正相關(guān)。
[Abstract]:The denudation particles of biological matrix samples were studied by 213 nm- nanosecond laser denudation system, and the laser denudation conditions were optimized. When the denudation energy is 25, the beam spot diameter is 200 渭 m, the denudation rate is 20 渭 m / s, the frequency is 20 Hz, the carrier gas is 700 m L He 700 m L Ar, the signal intensity and stability are the best. The relative fractionation factors of 56 elements were investigated under the optimum denudation conditions with ~ (31) P as the internal standard element. The results show that the denudation particles of the biological matrix are larger than the NIST 610 glass standard sample, and the fractionation effect of the elements in the biological matrix is smaller than that of the glass matrix, and the relative fractionation factor of most elements is 1.0 鹵0.1. The mechanism of element fractionation in biological matrix was discussed. The reason that the denudation particle of biological matrix was larger than that of glass substrate but the relative fractionation factor was not obviously increased was analyzed. On the one hand, it is possible that the particle size of 3 渭 m is atomized after entering the inductively coupled plasma; on the other hand, the enrichment effect of the large denudated particles is relatively small. It is found that the fractionation effect is related to the laser denudation energy, laser frequency and scanning rate, and negatively correlated with the oxide boiling point of the elements, and positively correlated with the oxide bond energy and ionization energy.
【作者單位】： 中國科學(xué)院上海硅酸鹽研究所;中國科學(xué)院大學(xué);
【基金】：國家自然科學(xué)基金項目(No.21175145) 中國科學(xué)院儀器設(shè)備功能開發(fā)技術(shù)創(chuàng)新項目(No.Y57YQ5150G)資助~~
【分類號】：O657.63;Q-33
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本文編號：2272474