發(fā)布時(shí)間：2018-10-09 15:07

【摘要】：目的:建立火把花根片中間體的高效液相色譜(HPLC)指紋圖譜。方法:采用HPLC法。色譜柱為用Inertsil ODS-4,流動(dòng)相為乙腈-0.1%磷酸溶液(梯度洗脫),流速為0.75 mL/min,檢測(cè)波長(zhǎng)為220 nm,柱溫為30℃,進(jìn)樣量為10μL。以雷公藤晉堿為參照,測(cè)定10批樣品的HPLC圖譜,采用《中藥色譜指紋圖譜相似度評(píng)價(jià)系統(tǒng)》(2004 A版)進(jìn)行共有峰指認(rèn)和相似度評(píng)價(jià)。結(jié)果:10批火把花根片中間體的HPLC圖譜有25個(gè)共有峰,相似度均0.90。經(jīng)驗(yàn)證,10批樣品HPLC圖譜與對(duì)照指紋圖譜具有較好的一致性。結(jié)論:該研究所建指紋圖譜可為火把花根片中間體的鑒別和質(zhì)量評(píng)價(jià)提供參考。
[Abstract]:Objective: to establish the (HPLC) fingerprint of the intermediate of Huanghuatou root tablets by high performance liquid chromatography (HPLC). Methods: HPLC method was used. The chromatographic column consisted of Inertsil ODS-4, mobile phase with acetonitrile-0.1% phosphoric acid solution (gradient elution), the flow rate was 0.75 mL/min, the detection wavelength was 220 nm, the temperature was 30 鈩，

本文編號(hào)：2259786