發(fā)布時間：2018-08-24 15:53

【摘要】：建立了制備型高效液相色譜(Prep-HPLC)分離高良姜黃酮中高良姜素和山柰素的方法。高良姜黃酮經(jīng)HPD-600樹脂吸附洗脫純化后,采用Prep-HPLC分離高良姜黃酮中高良姜素和山柰素。制備色譜條件:流動相為甲醇-0.6%(v/v)乙酸水溶液(58∶42,v/v),柱溫為常溫,流速為7.0 mL/min,檢測波長為360 nm,進樣量為700μL,上樣質(zhì)量濃度為10.0 g/L。分離的單體由質(zhì)譜和核磁共振氫譜、碳譜鑒定確證為高良姜素和山柰素,HPLC外標法定量,純度分別為99.5%和99.7%。該方法分離效果好、高效、低毒,可用于高良姜中高良姜素和山柰素的分離制備。
[Abstract]:A method for the separation of galangin and kaempferol from flavonoids of alpinia officinalis by Prep-HPLC was established. The flavonoids of alpinia officinalis were eluted and purified by HPD-600 resin, and Prep-HPLC was used to separate alpinol and kaempferol from the flavonoids of alpinia officinalis. The chromatographic conditions were as follows: the mobile phase was methanol-0.6% (v / v) acetic acid solution (58: 42 v / v), the column temperature was normal temperature, the flow rate was 7.0 mL/min, the detection wavelength was 360 nm, the sample volume was 700 渭 L, and the sample concentration was 10.0 g / L. The isolated monomers were identified by mass spectrometry and nuclear magnetic resonance (NMR) and identified as gingerin and kaempferol by HPLC. The purity was 99.5% and 99.7%, respectively. The method has the advantages of good separation, high efficiency and low toxicity. It can be used for the separation and preparation of galangin and kaempferol from alpinia officinalis.
【作者單位】： 茂名職業(yè)技術(shù)學院;廣東中輕楓泰生化科技有限公司;華南理工大學食品科學與工程學院;
【基金】：茂名市科技計劃項目([2015]14)~~
【分類號】：O652.63;R284

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