【摘要】：目的建立10個采收期(3月末、5月初、7月初、8月末、9月初、9月末、10月初、10月末、11月初、11月中)青錢柳Cyclocarya paliurus(Batal)Iljinsk的HPLC指紋圖譜,并測定4種成分含有量。方法青錢柳80%甲醇提取液的分析采用Agilent C18色譜柱(5μm,250 mm×4.6 mm);乙腈-0.1%磷酸為流動相,梯度洗脫;檢測波長365nm(指紋圖譜)和254 nm(含有量測定);體積流量1.0 m L/min;柱溫35℃。再對指紋圖譜中的共有峰進行主成分分析。結(jié)果 10批樣品指紋圖譜中有15個共有峰,相似度0.920~0.981,4個主成分累計貢獻率達92.82%,峰1、2(綠原酸)、12、14的權(quán)重值較大。綠原酸、異槲皮苷、槲皮素、山柰酚分別在0.008 492~0.566 1、0.004 370~0.291 3、0.000 551 5~0.036 77、0.001 287~0.085 77 mg/m L范圍內(nèi)線性關(guān)系良好,平均加樣回收率分別為98.2%、97.5%、96.3%、97.0%,RSD分別為1.35%、1.14%、2.21%、1.74%。各采收期樣品中4種成分含有量差異明顯,其中綠原酸與異槲皮苷在7月初最高,而槲皮素和山柰酚分別在8月末和9月初最高。結(jié)論該方法可用于青錢柳的質(zhì)量控制,并為篩選其最佳采收期提供依據(jù)。
[Abstract]:Objective to establish the HPLC fingerprint of Cyclocarya paliurus (Batal) Iljinsk in 10 harvest periods (from the end of March to the beginning of July to the beginning of September and early October to the beginning of October and November), and to determine the contents of four components. Methods Agilent C18 column (5 渭 m ~ 250mm 脳 4.6 mm); acetonitrile-0.1% phosphoric acid) was used as mobile phase for the analysis of 80% methanol extract from Salix Cycariae, the detection wavelength was 365nm (fingerprint) and 254nm (content determination), the volume flow rate was 1.0 mL / min, the column temperature was 35 鈩，

本文編號：2197866