【摘要】：目的:建立同時測定茯苓中去氫土莫酸、去氫茯苓酸、茯苓酸和松苓新酸含量的HPLC方法。方法:色譜柱為Wonda Cract C_(18)柱(150 mm×4.6 mm,5μm),以乙腈-0.2%甲酸溶液(76∶24)為流動相,檢測波長222 nm,流速1.0 m L/min,柱溫32℃。結(jié)果:4種活性成分均達到基線分離,線性關(guān)系良好(r≥0.9998);平均加樣回收率分別為100.26%、88.71%、108.43%、101.70%。結(jié)論:該方法靈敏、準確、可靠、重復(fù)性好,可用于茯苓藥材的質(zhì)量控制和體內(nèi)活性評價。
[Abstract]:Objective: to establish a HPLC method for simultaneous determination of deshydrotomic acid, dehydroporic acid, Poria cocoic acid and pachymaric acid in Poria cocos. Methods: the chromatographic column was Wonda Cract C18 column (150mm 脳 4.6mm ~ 5 渭 m),) with acetonitrile-0.2% formic acid solution (76:24) as mobile phase, detection wavelength was 222nm, flow rate was 1.0 mL / min, column temperature was 32 鈩，

本文編號：2196020