【摘要】：雙酚A作為一種環(huán)境雌激素,對人類以及其他生命體具有很大的危害。當(dāng)雙酚A進(jìn)入生命機(jī)體后,會干擾正常的內(nèi)分泌物的合成,造成機(jī)體內(nèi)各大系統(tǒng)的紊亂,從而產(chǎn)生各種疾病危害健康,甚至影響人類繁衍。因此,分離檢測雙酚A有著重要意義。近年來,管內(nèi)固相微萃取用于分離檢測痕量雙酚A,由于其在實現(xiàn)自動化、低成本、環(huán)境友好等方面的優(yōu)勢得到了蓬勃發(fā)展。然而,這些萃取介質(zhì)對雙酚A沒有特異選擇性,容易受到基質(zhì)或共存物干擾,影響分析準(zhǔn)確性和靈敏度。表面分子印跡技術(shù)制備的聚合物對目標(biāo)分子具有一定的選擇性,在雙酚A的分離檢測中也得到了廣泛的應(yīng)用。然而,表面分子印跡聚合物也存在一定的非特異性吸附。適配體作為一種高特異性識別材料在雙酚A的特異性檢測方面,已經(jīng)得到廣泛運(yùn)用。本論文結(jié)合管內(nèi)固相微萃取、表面分子印跡技術(shù)以及適配體的高特異性識別能力,對雙酚A的高效分離與檢測進(jìn)行了研究,為雙酚A的分離與檢測提供了一定的參考價值。具體研究內(nèi)容如下:1、通過適配體親和雙酚A定軌表面印跡技術(shù)制備了特異性識別雙酚A的分子印跡聚合物。對獲得的適配體-表面分子印跡聚合物微球和普通的表面分子印跡聚合物微球的吸附性能和選擇性能進(jìn)行了測試比較,實驗發(fā)現(xiàn)適配體-表面分子印跡聚合物微球,這種印跡方式提高了模板利用率,可以形成更密集的氫鍵的位點。適配體表面分子印跡的吸附能力是普通表面分子印跡的兩倍,且印跡孔穴中的適配體能對目標(biāo)分子具有較高的選擇性。這表明,適配體結(jié)合表面分子印跡有望成為一個很好選擇性提取材料。2、制備了一種雙重識別的共振光傳感器,用于檢測雙酚A。這種雙重識別來源于適配體和表面分子印跡的共同作用。試驗過程中考察了不同條件(pH,時間,粒子用量,溫度等)對共振光響應(yīng)值的影響。測試了它的選擇性和相對標(biāo)準(zhǔn)偏差等。這種以納米硅材料為主體交聯(lián)適配體的分子印跡共振光傳感器,制備過程簡單經(jīng)濟(jì),對檢測雙酚有一定的參考價值。3、以雙酚A為分析對象,在適宜的管狀材料上,通過電聚合方法制備結(jié)構(gòu)和厚度可控的、具有高特異選擇性的表面分子印跡聚合物膜。研究電聚合過程各因素對分子印跡聚合物結(jié)構(gòu)以及性能的影響,探討其構(gòu)效關(guān)系。為后續(xù)將該表面分子印跡管連接到高效液相色譜儀上,構(gòu)建管內(nèi)固相微萃取—高效液相色譜聯(lián)用分析系統(tǒng),建立在線選擇性分離富集環(huán)境水樣中的BPA的方法做個鋪墊。
[Abstract]:Bisphenol A, as an environmental estrogen, is very harmful to human beings and other living organisms. When bisphenol A enters into living organisms, it will interfere with the synthesis of normal endocrine substances, causing disorders in the body's major systems, resulting in a variety of diseases that endanger health, and even affect human reproduction. In recent years, in-tube solid-phase microextraction (SPME) for the separation and detection of trace bisphenol A (BPA) has been developed rapidly due to its advantages of automation, low cost and environmental friendliness. The polymer prepared by imprinting technique has certain selectivity to the target molecule and has been widely used in the separation and detection of bisphenol A. However, the surface molecularly imprinted polymer also has some nonspecific adsorption. As a highly specific recognition material, aptamer has been widely used in the detection of bisphenol A specificity. In this paper, combined with in-tube solid-phase microextraction, surface molecularly imprinted technique and high specific recognition ability of aptamer, the separation and detection of bisphenol A were studied, which provided a certain reference value for the separation and detection of bisphenol A. Molecularly imprinted polymers for specific recognition of bisphenol A were prepared. The adsorption and selectivity of the obtained aptamer-surface molecularly imprinted polymer microspheres were tested and compared with those of the conventional surface molecularly imprinted polymer microspheres. It was found that the aptamer-surface molecularly imprinted polymer microspheres improved the template efficiency. The molecular imprinting ability of the aptamer surface is twice that of the common surface molecule imprinting, and the aptamer in the imprinting hole has high selectivity to the target molecule. This indicates that the aptamer-binding surface molecule imprinting is expected to be a good selective extraction material. A dual-recognition resonant light sensor for detecting bisphenol A. The dual recognition results from the interaction of aptamer and surface molecular imprinting. The effects of different conditions (pH, time, particle dosage, temperature, etc.) on the resonant light response were investigated. The selectivity and relative standard deviation of the sensor were measured. Molecularly imprinted polymer films with controllable structure and thickness were prepared by electropolymerization on suitable tubular materials with bisphenol A as analytical object. The effects of various factors on the structure and properties of molecularly imprinted polymers (MIPs) during electropolymerization were studied, and their structure-activity relationships were discussed. Make a paving the way.
【學(xué)位授予單位】：湘潭大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O631.3;X832

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 Underwood Susanne W.;Jong Edmund C.;Luxbacher Kray D.;Sarver Emily A.;Ripepi Nino S.;Mc Nair Harold M.;;Solid phase microextraction(SPME) sampling under turbulent conditions and for the simultaneous collecting of tracer gases[J];International Journal of Mining Science and Technology;2015年04期

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本文編號：2194363