【摘要】：金屬納米簇是近年來發(fā)展起來的一類粒徑小于或接近于費子波爾半徑的半導(dǎo)體納米顆粒,因為其獨特的光、電學(xué)性質(zhì)以及良好的生物相容性、水溶性、低毒性等優(yōu)點,廣泛應(yīng)用于化學(xué)傳感、生物標(biāo)記、疾病檢測等分析檢測領(lǐng)域。本文以聚乙烯亞胺為模板的銀納米簇為研究對象,構(gòu)建了檢測喹諾酮類抗生素、維生素B12、目標(biāo)DNA和三磷酸腺苷(adenosine triphosphate,ATP)的新方法。主要研究內(nèi)容如下:(1)以不同分子量(Mw=600、1300、2000、10000、25000、70000和750000)的聚乙烯亞胺(polyethyleneimine,PEI)為模板合成的銀納米簇(Ag nanoclusters,Ag NCs)為熒光探針,當(dāng)向Ag NCs中添加Cu2+,隨著Cu2+濃度的增大使得Ag NCs的熒光強(qiáng)度逐漸猝滅,通過向其系統(tǒng)中加入喹諾酮類藥物(包括萘啶酸、西諾沙星、環(huán)丙沙星、氧氟沙星、莫西沙星)使得Ag NCs的熒光逐漸恢復(fù)。同時,這一傳感系統(tǒng)(Ag NCs-Cu2+)對上述喹諾酮類藥物的檢測顯示出了良好的線性范圍和較低的檢出限。此外,該傳感器系統(tǒng)已成功用于人血清和尿中喹諾酮類藥物的測定。(2)以不同分子量(Mw=600、10000和70000)以及不同封端基團(tuán)(乙二胺和乙氧基)的PEI為模板合成的Ag NCs作為一種新型熒光探針,隨著維生素B12濃度的增大,由于內(nèi)濾效應(yīng),其可以猝滅Ag NCs的熒光。同時,該方法選擇性好、靈敏度高,已被成功用于注射劑和片劑樣品中維生素B12的測定。(3)血紅素適配體與血紅素可形成復(fù)合物,該復(fù)合物與Ag NCs之間發(fā)生光誘導(dǎo)電子轉(zhuǎn)移(photoinduced electron transfer,PET)猝滅Ag NCs的熒光。以此為實驗機(jī)理,以分子量為600的PEI包裹的Ag NCs作為熒光探針,本章構(gòu)建了具有廣泛適用性的傳感平臺,通過改變DNA序列,建立了檢測目標(biāo)DNA和ATP的分析方法。以目標(biāo)DNA/ATP和血紅素作為輸入信號,以Ag NCs的熒光變化作為輸出信號,構(gòu)建熒光“AND”邏輯門。
[Abstract]:Metal nanoclusters are a kind of semiconductor nanoparticles whose particle size is less than or close to the Fertilizer radius developed in recent years, because of its unique light, electrical properties and good biocompatibility, water solubility, low toxicity and so on. Widely used in chemical sensing, biomarker, disease detection and other analytical field. In this paper, a new method for the detection of quinolones, vitamin B12, target DNA and adenosine triphosphate ATP was constructed with polyimide as the template of silver nanoclusters. The main research contents are as follows: (1) Ag nanoclusters (Ag nanoclusters Ag NCs) with different molecular weights (MwC600,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000@@ The fluorescence of Ag NCs was gradually restored by adding quinolones (including naphthalic acid, ciprofloxacin, ofloxacin, moxifloxacin) into the system. At the same time, this sensing system (Ag NCs-Cu2) showed good linear range and low detection limit for quinolones. In addition, the sensor system has been successfully used for the determination of quinolones in human serum and urine. (2) Ag NCs with different molecular weights (MwC600,10000 and 70000) and PEI with different entrapment groups (ethylenediamine and ethoxy) as a new fluorescent probe, has been successfully used for the determination of quinolones in human serum and urine. With the increase of vitamin B12 concentration, the fluorescence of Ag NCs can be quenched due to the internal filtration effect. At the same time, the method has good selectivity and high sensitivity. It has been successfully used for the determination of vitamin B12 in injection and tablet samples. (3) heme aptamer can form complex with heme. The fluorescence of Ag NCs was quenched by photo-induced electron transfer (photoinduced electron transfer PET between the complex and Ag NCs. Based on the experimental mechanism, using Ag NCs encapsulated by PEI with molecular weight 600 as fluorescence probe, a widely applicable sensing platform is constructed in this chapter. By changing the DNA sequence, an analytical method for detecting target DNA and ATP is established. The target DNA/ATP and heme were used as input signals, and the fluorescence changes of Ag NCs were used as output signals to construct the fluorescent "AND" logic gate.
【學(xué)位授予單位】：曲阜師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R927;O657.3;TQ460.72

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