發(fā)布時(shí)間：2018-08-09 19:20

【摘要】：目的:建立超高效液相色譜法分離和測(cè)定不同收集期的香蕉和皇帝蕉皮及其果肉中羽扇豆酮含量的方法,并優(yōu)化提取條件。方法:采用甲醇回流提取法對(duì)2種蕉皮及其果肉的羽扇豆酮進(jìn)行提取分離,減壓濃縮,選用Waters ACQUITY UPLC BEH C18色譜柱(2.1 mm×50 mm,1.7μm),流動(dòng)相為乙腈-水(85∶15,V/V)溶液,流速0.3 mL/min,柱溫30℃,檢測(cè)波長(zhǎng)206 nm。結(jié)果:香蕉和皇帝蕉果肉中均未檢測(cè)出羽扇豆酮,但2種蕉皮中有較高含量的羽扇豆酮,并且在16.70~59.96 mg/100 mL質(zhì)量濃度范圍內(nèi)有良好線性關(guān)系(r=0.999 6),羽扇豆酮平均回收率為99.00%,相對(duì)標(biāo)準(zhǔn)偏差為3.0%。結(jié)論:該法回收率高、重復(fù)性好,且具有專屬性強(qiáng)、準(zhǔn)確性高和簡(jiǎn)便快捷等特點(diǎn),適用于香蕉、皇帝蕉皮中羽扇豆酮的含量測(cè)定。
[Abstract]:Objective: to establish a high performance liquid chromatography method for the separation and determination of Lupanone in banana, banana peel and pulp of banana and emperor in different collecting periods, and to optimize the extraction conditions. Methods: two kinds of plantain peel and pulp were extracted and concentrated by methanol reflux extraction method. Waters ACQUITY UPLC BEH C18 column (2.1 mm 脳 50 mm ~ (1.7) 渭 m),) was used as mobile phase with acetonitrile-water (85: 15 V / V) solution, flow rate was 0.3 mL / min, column temperature was 30 鈩，

本文編號(hào)：2175065