高靈敏表面增強拉曼襯底的研制
發(fā)布時間：2018-08-03 09:57
【摘要】：自從表面增強拉曼散射(SERS)被發(fā)現(xiàn)以來,SERS光譜技術因其高靈敏、快速、高效、簡單的優(yōu)點被廣泛應用于生物、醫(yī)學、化學分析等領域。特別是當目標分子數(shù)量低于ppb級別時,SERS光譜的優(yōu)勢顯得尤為突出,它可以在復雜的體系中檢測到生物化學類樣品的指紋特征拉曼譜,可用于檢測、鑒定甚至標定含量。近年來我國的食品安全問題時有發(fā)生,簡單高效的SERS光譜技術在食品安全檢測中發(fā)揮了重要的作用。將SERS技術應用于食品安全檢測最關鍵的是制備出經(jīng)濟、增強效果好、可重復性高的SERS襯底。本論文利用FDTD方法對金字塔、截角金字塔、橢球體、方塊、米字形和點陣形貌金襯底的散射光譜和空間電場分布進行了理論模擬計算,分析了襯底的表面等離子體共振特性,研究了各自的SERS效果。模擬結果表明將襯底表面微形貌化會產(chǎn)生較好的增強效果,并且復雜的形貌比單一的形貌增強效果更好,同時形貌的尺寸改變時,散射光譜的規(guī)律也會發(fā)生變化。實驗中利用電子束曝光和軟模板壓印技術成功制備出了米字形和點陣形貌的金襯底,并將其用于胱氨酸和三聚氰胺的拉曼散射光譜的測量,測量結果表明米字形金襯底和點陣金襯底都能檢測到濃度為50ppm的胱氨酸的拉曼特征峰,并且米字形金襯底也可以檢測到濃度為50ppm的三聚氰胺的拉曼特征峰。
[Abstract]:Since the discovery of surface-enhanced Raman scattering (SERS) spectroscopy technology has been widely used in biological, medical, chemical analysis and other fields because of its high sensitivity, fast, high efficiency and simple advantages. Especially when the number of target molecules is lower than the ppb level, the advantage of SERS spectrum is particularly obvious. It can detect the characteristic Raman spectrum of biochemistry samples in complex system, and can be used to detect, identify and even calibrate the content. In recent years, food safety problems occur from time to time in our country. Simple and efficient SERS spectrum technology plays an important role in food safety detection. The key to the application of SERS in food safety detection is to prepare SERS substrates which are economical, effective and reproducible. In this paper, the scattering spectra and spatial electric field distribution of gold substrates with pyramid, truncated pyramid, ellipsoid, block, meter shape and lattice topography are simulated by FDTD method. The surface plasmon resonance characteristics of the substrate are analyzed. The effects of SERS were studied. The simulation results show that the microtopography of the substrate surface will produce a better enhancement effect, and the complex morphology is better than the single morphology enhancement effect. At the same time, when the size of the morphology changes, the law of the scattering spectrum will also change. In the experiment, the gold substrates with meter shape and lattice morphology were successfully prepared by electron beam exposure and soft template imprint techniques, and used to measure the Raman scattering spectra of cystine and melamine. The results show that the Raman characteristic peaks of cystine at the concentration of 50ppm can be detected on the meter shaped gold substrate and the lattice gold substrate, and the Raman characteristic peak of melamine at the concentration of 50ppm can also be detected on the meter shaped gold substrate.
【學位授予單位】：南京郵電大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.37
本文編號：2161383
[Abstract]:Since the discovery of surface-enhanced Raman scattering (SERS) spectroscopy technology has been widely used in biological, medical, chemical analysis and other fields because of its high sensitivity, fast, high efficiency and simple advantages. Especially when the number of target molecules is lower than the ppb level, the advantage of SERS spectrum is particularly obvious. It can detect the characteristic Raman spectrum of biochemistry samples in complex system, and can be used to detect, identify and even calibrate the content. In recent years, food safety problems occur from time to time in our country. Simple and efficient SERS spectrum technology plays an important role in food safety detection. The key to the application of SERS in food safety detection is to prepare SERS substrates which are economical, effective and reproducible. In this paper, the scattering spectra and spatial electric field distribution of gold substrates with pyramid, truncated pyramid, ellipsoid, block, meter shape and lattice topography are simulated by FDTD method. The surface plasmon resonance characteristics of the substrate are analyzed. The effects of SERS were studied. The simulation results show that the microtopography of the substrate surface will produce a better enhancement effect, and the complex morphology is better than the single morphology enhancement effect. At the same time, when the size of the morphology changes, the law of the scattering spectrum will also change. In the experiment, the gold substrates with meter shape and lattice morphology were successfully prepared by electron beam exposure and soft template imprint techniques, and used to measure the Raman scattering spectra of cystine and melamine. The results show that the Raman characteristic peaks of cystine at the concentration of 50ppm can be detected on the meter shaped gold substrate and the lattice gold substrate, and the Raman characteristic peak of melamine at the concentration of 50ppm can also be detected on the meter shaped gold substrate.
【學位授予單位】：南京郵電大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.37
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，本文編號：2161383
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