【摘要】：建立了二維柱切換-超高效液相色譜法同時測定明目地黃丸中莫諾苷、馬錢苷、芍藥苷、丹皮酚含量的方法。一維色譜柱為Thermo Accucore XL C_(18)(250 mm×2.1 mm,4μm),二維色譜柱為DIONEX Acclaim phenyl-1(150 mm×4.6 mm,3μm),一維分析流動相為乙腈-水,梯度洗脫,二維分析流動相為乙腈-水,等度洗脫,14.5 min進(jìn)行閥切換;檢測波長:0~14.5 min為240 nm,14.5~30 min為275 nm,流速:0.5 m L/min,柱溫:30℃。30 min即可完成明目地黃丸中莫諾苷、馬錢苷、芍藥苷、丹皮酚的含量測定,并有效地將莫諾苷同分異構(gòu)體分離。莫諾苷、馬錢苷、芍藥苷、丹皮酚的線性范圍分別為7.6~377 mg/L,9.2~459 mg/L,8.4~419 mg/L和8.2~409 mg/L,相關(guān)系數(shù)為0.9999,加樣回收率為98.3%~100.2%。本方法快捷高效,可對控制明目地黃丸質(zhì)量提供參考。
[Abstract]:A method for simultaneous determination of monoside, marganin, paeoniflorin and paeonol in Mingmu Rehmannia pills by two dimensional column switching and ultra-high performance liquid chromatography was established. One dimensional column was Thermo Accucore XL C _ (18) (250mm 脳 2.1mm ~ 4 渭 m),). Two-dimensional column was DIONEX Acclaim phenyl-1 (150mm 脳 4.6mm ~ 3 渭 m),). The mobile phase was acetonitrile-water, gradient elution was gradient elution. The mobile phase was acetonitrile-water, and the isometric elution was 14.5 min for valve switching. The detection wavelength of 14.5 min was 240nmL 14.5 min, the flow rate was 275nm / min, the column temperature was 30 鈩，

本文編號：2146134