本文選題：熒光探針 + 熒光基團(tuán)�。� 參考：《應(yīng)用化學(xué)》2017年12期

【摘要】：正熒光技術(shù)已發(fā)展成為分子生物學(xué)、細(xì)胞生物學(xué)、分析化學(xué)、環(huán)境檢測(cè)和臨床診斷的有力工具,具有靈敏度高,選擇性好,時(shí)空分辨率高,設(shè)備簡(jiǎn)單廉價(jià)等突出優(yōu)勢(shì)。熒光探針通常由熒光基團(tuán)和識(shí)別基團(tuán)組成。識(shí)別基團(tuán)可以選擇性感知環(huán)境參數(shù),也可以識(shí)別小分子、離子、酶、蛋白、核酸等。識(shí)別事件如何產(chǎn)生熒光響應(yīng),需要研究熒光發(fā)射的基本規(guī)律。當(dāng)染料吸收光到達(dá)激發(fā)態(tài)后,其能量可以熒光形式釋放,也可以被內(nèi)轉(zhuǎn)換、電子轉(zhuǎn)移、能量轉(zhuǎn)移、或系間穿越等過(guò)程耗散。識(shí)別事件通過(guò)對(duì)上述激發(fā)態(tài)能量釋放
[Abstract]:Positive fluorescence technology has been developed into a powerful tool for molecular biology, cell biology, analytical chemistry, environmental detection and clinical diagnosis. It has the advantages of high sensitivity, good selectivity, high space-time resolution, simple and cheap equipment and so on. Fluorescence probes usually consist of fluorescent groups and recognition groups. Recognition groups can choose sexy environmental parameters, but also recognize small molecules, ions, enzymes, proteins, nucleic acids and so on. The basic rules of fluorescence emission need to be studied to identify how the event produces fluorescence response. When the dye absorbs light to the excited state, its energy can be released in the form of fluorescence or dissipated by internal conversion, electron transfer, energy transfer, or intersystem crossing. Identify the event by releasing the energy of the excited state
【作者單位】： 大連理工大學(xué)精細(xì)化工國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【分類(lèi)號(hào)】：O657.3
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本文編號(hào)：2113000