本文選題：滇龍膽 + 灰色關聯(lián)度分析法�。� 參考：《中國實驗方劑學雜志》2017年13期

【摘要】：目的:測定不同來源滇龍膽樣品中4種指標成分,結合灰色關聯(lián)度分析(GRA)法建立其質(zhì)量評價方法。方法:采用高效液相色譜法測定云南10個不同地區(qū)的50株滇龍膽根及根莖中主要藥效成分馬錢苷酸、獐牙菜苦苷、龍膽苦苷和當藥苷的含量�；贕RA法處理樣品各指標成分數(shù)據(jù),計算各評價單元序列相對于參考序列的相對關聯(lián)度作為測度,對樣品進行綜合評價。結果:建立滇龍膽樣品的指紋圖譜,并測定出4種主要成分的含量。滇龍膽樣品的相對關聯(lián)度r_i在0.306~0.600,其中14個樣品的r_i0.500,23個樣品的r_i0.400,表明樣品質(zhì)量之間存在較大差異。大理鶴慶的DH3和DH5的r_i分別為0.600和0.576,質(zhì)量最優(yōu)。不同地區(qū)樣品r_i的平均值在0.325~0.541,鶴慶樣品r_i的平均值最大,質(zhì)量總體水平最高,可作為優(yōu)質(zhì)種源進一步研究和推廣。各地區(qū)樣品r_i的RSD在4.4%~11.9%,昆明尋甸樣品r_i的RSD最小,樣品最為穩(wěn)定。結論:GRA法結合多指標成分定量的評價方法簡單、全面,可用于滇龍膽的質(zhì)量評價,為資源開發(fā)利用及優(yōu)質(zhì)種源的篩選提供了參考依據(jù)。
[Abstract]:Objective: to determine the four index components in samples from different sources of Gentiana and to establish a method for quality evaluation with the method of grey correlation analysis (GRA). Methods: HPLC was used to determine the contents of maqouside, Swertia, gentiopicrin and danamycin in the root and rhizome of 50 Gentianopsis yunnanensis in 10 different regions of Yunnan. Based on the GRA method, the relative correlation degree of each evaluation unit sequence relative to the reference sequence is calculated as a measure to evaluate the sample synthetically. Results: the fingerprint of Gentiana chinensis was established and the contents of four main components were determined. The relative correlation degree of Gentiana yunnanensis samples is 0.306 ~ 0.600, of which 14 samples are r _ S _ I _ (0.500) and 23 samples are r _ s _ I _ (0.400), which indicates that there is a great difference in the quality of the samples. The RCI of DH3 and DH5 in Heqing, Dali, was 0.600 and 0.576, respectively. The quality of DH3 and DH5 was the best. The average value of the samples of different regions was 0.325 ~ 0.541.The average value of the samples of Heqing was the largest and the quality of the samples was the highest, which could be used as a good provenance for further study and popularization. The RSD of the samples of each region was 4.4 and 11.9.The RSD of the Kunming Xundian sample was the smallest and the sample was the most stable. Conclusion the weight GRA method is simple and comprehensive, and can be used to evaluate the quality of Gentiana paniculata. It provides a reference for resource exploitation and selection of high quality provenances.
【作者單位】： 云南中醫(yī)學院中藥學院;云南省農(nóng)業(yè)科學院藥用植物研究所;云南省省級中藥原料質(zhì)量監(jiān)測技術服務中心;
【基金】：國家自然科學基金項目(81660638,81260608) 云南省自然科學基金項目(2013FZ150,2014FD068)
【分類號】：O657.72;R284.1
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本文編號：2104339