本文選題：南極磷蝦油 + 砷　； 參考：《分析化學(xué)》2016年05期

【摘要】：南極磷蝦油富含Omega-3磷脂而具有強(qiáng)大的保健功能,但由于其砷含量較高,在保健品領(lǐng)域的應(yīng)用受到一定限制。由于南極磷蝦油中含有大量的磷脂質(zhì),采用常規(guī)的提取方法分析難以對(duì)其砷形態(tài)進(jìn)行準(zhǔn)確定量分析。本研究采用高效液相色譜-電感耦合等離子體質(zhì)譜((HPLC-ICP-MS)聯(lián)用技術(shù)建立了針對(duì)砷甜菜堿(As B)、二甲基砷酸(DMA)、一甲基砷酸(MMA)、三價(jià)砷As(Ⅲ)、五價(jià)砷As(Ⅴ)5種砷形態(tài)的分析方法。采用C_(18)固相萃取進(jìn)行樣品脫脂前處理,采用陰離子交換色譜Ion Pac AS9-H陰離子分析柱(250 mm×4 mm),流動(dòng)相采用38 mmol/L Na_2SO_4+15 mmol/L乙酸鈉;流速0.6 m L/min,流速1 m L/min,進(jìn)行5種形態(tài)砷化合物的分析。結(jié)果表明,5種砷化合物的線(xiàn)性范圍為0.5~500μg/L,相關(guān)系數(shù)R0.9993.檢出限(LOD)為0.1~0.2μg/L,定量限(LOQ)為1.5~2.6μg/L,RSD5%。加標(biāo)回收率為88.9%~106.3%。樣品的測(cè)定結(jié)果顯示,南極磷蝦油中砷形態(tài)的主要存在形式為無(wú)毒的砷甜菜堿,因此可以安全地應(yīng)用于食品及保健品。
[Abstract]:Antarctic krill oil is rich in Omega-3 phospholipids and has powerful health care function, but its application in the field of health products is limited due to its high arsenic content. Due to the large amount of phospholipid in Antarctic krill oil, it is difficult to analyze the arsenic species accurately by conventional extraction method. High performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) was used to determine the arsenic speciation of arsenic betaine (as B), dimethyl arsenic acid (DMA), monomethylarsenic acid (MMA), trivalent arsenic as (鈪，

本文編號(hào)：2073306