本文選題：痹通藥酒 + 新烏頭堿　； 參考：《中成藥》2017年12期

【摘要】：目的建立HPLC法同時測定痹通藥酒中4種生物堿的含有量。方法該藥物乙醚提取液分析采用Agilent C18色譜柱(5μm,4.6 mm×250 mm);流動相[乙腈-四氫呋喃(25∶15)]-0.1 mol/L醋酸銨,梯度洗脫;體積流量1.0 m L/min;柱溫30℃;檢測波長235 nm。結果新烏頭堿、次烏頭堿、烏頭堿、苯甲酰新烏頭原堿分別在39.87~199.36、99.63~498.13、13.87~69.33、39.65~198.27μg/m L范圍內線性關系良好(r0.999 0),平均加樣回收率分別為98.24%(RSD=0.75%)、99.54%(RSD=0.50%)、97.60%(RSD=1.54%)、99.52%(RSD=1.32%)。結論該方法簡便、準確、可靠,可用于痹通藥酒的質量評價和控制。
[Abstract]:Objective to establish a HPLC method for simultaneous determination of four alkaloids in Bitong medicinal wine. Methods the Agilent C18 column (4.6 mm 脳 250 mm);) was used for the analysis of the ether extract. The mobile phase [acetonitrile-tetrahydrofuran (25:15)] -0.1 mol / L ammonium acetate was used for gradient elution, the volume flow rate was 1.0 mL / min, the column temperature was 30 鈩，

本文編號：2066404