發(fā)布時(shí)間：2018-06-18 03:35

  本文選題：雙親水性嵌段共聚物 + 熒光化學(xué)傳感器��； 參考：《江蘇大學(xué)》2017年碩士論文

【摘要】：近年來,自組裝的概念已經(jīng)被引入到熒光化學(xué)傳感器的領(lǐng)域中,通過利用智能型聚合物作為響應(yīng)的基體,制備新型熒光化學(xué)傳感器能夠?qū)崿F(xiàn)多種功能的協(xié)同性以及更廣泛的適應(yīng)性。本課題利用了溫度響應(yīng)型雙親水性嵌段共聚物的自組裝來設(shè)計(jì)和制備具有良好水溶性、溫度響應(yīng)性、金屬離子或pH響應(yīng)性、生物相容性、高靈敏性等集多種功能于的一體新型熒光化學(xué)傳感器。(1)制備了一種新型基于雙親水性嵌段共聚物的金屬離子熒光化學(xué)傳感器,PEG113-b-P(NIPAM-co-SHMa)65,在溫敏性嵌段中標(biāo)記了金屬離子識(shí)別基元SHMa。它可以作為金屬離子和溫度響應(yīng)的多功能熒光化學(xué)傳感器,不僅能夠從眾多金屬離子中選擇性識(shí)別Al3+和Zn2+,根據(jù)熒光顏色和熒光響應(yīng)時(shí)間的不同來區(qū)別Al3+和Zn2+,而且具有很強(qiáng)的抗干擾能力、極佳的水溶性、較高的靈敏度和出色的生物相容性。一旦溫度升高會(huì)導(dǎo)致溫敏嵌段PNIPAM坍塌,DHBCs在水溶液中隨即發(fā)生自組裝,金屬離子熒光響應(yīng)基元SHMa將聚集到極性較小的疏水核里面,量子產(chǎn)率繼而提高,檢測(cè)靈敏度進(jìn)一步提升。另外,回收率實(shí)驗(yàn)證明PEG113-b-P(NIPAM-co-SHMa)65可用于實(shí)際生物制品中Al3+或Zn2+的定性和定量檢測(cè)。(2)制備了一種新型的基于雙親水性嵌段共聚物的pH熒光化學(xué)傳感器,PEG113-b-P(NIPAM-co-SHMa-co-Rh6GEMa)62,并在溫敏性嵌段中標(biāo)記了堿性識(shí)別基元SHMa和酸性識(shí)別基元RhSAMa。它可以作為pH響應(yīng)和溫度響應(yīng)的多功能熒光化學(xué)傳感器,具有寬pH檢測(cè)范圍、溫度響應(yīng)性、水溶性、生物相容性、高靈敏度等特點(diǎn)。溫度升高超過其LCST溫度時(shí),pH的檢測(cè)信號(hào)可進(jìn)一步增強(qiáng)。同時(shí),通過自主開發(fā)的用于顏色識(shí)別的手機(jī)APP處理圖像獲取不同pH值下溶液的色差圖及色差圖數(shù)據(jù)ΔR,ΔG,ΔB。將pH 1-6和pH 8-14下溶液的ΔR,ΔG,ΔB數(shù)據(jù)分別利用SPSS 20建立主成份線性回歸定量模型用于定量待測(cè)溶液的pH。最后,利用主成份線性回歸定量模型開發(fā)了ColorExtract即時(shí)pH檢測(cè)手機(jī)APP。(3)制備了兩種新型基于雙親水性嵌段聚合物的金屬離子熒光化學(xué)傳感器,PEG113-b-P(NIPAM-co-BDMa)60,PEG113-b-P(NIPAM-co-R6GEMa)67,并分別在溫敏性嵌段中標(biāo)記Al3+和Fe3+識(shí)別基元BDMa和Rh6GEMa。它們組成的混合物可以作為溫度和混合金屬離子響應(yīng)的多功能熒光化學(xué)傳感器,而且具有很強(qiáng)的抗干擾能力、極佳的水溶性、較高的靈敏度和出色的生物相容性。此外,溫度誘導(dǎo)嵌段坍塌后,形成以P(NIPAM-co-BDMa)和P(NIPAM-co-R6GEMa)為核的混合膠束,可以進(jìn)一步提高其對(duì)Al3+和Fe3+的檢測(cè)靈敏度。同時(shí),本章通過自主開發(fā)的用于顏色識(shí)別的手機(jī)APP處理圖像以獲取不同Al3+和Fe3+混合比例下溶液反應(yīng)前后的色差圖及色差圖數(shù)據(jù)ΔR,ΔG,ΔB,利用SPSS 20建立了對(duì)Al3+和Fe3+混合金屬離子比例定量檢測(cè)的主成份線性回歸模型并對(duì)模型進(jìn)行了驗(yàn)證。
[Abstract]:In recent years, the concept of self-assembly has been introduced into the field of fluorescent chemical sensors by using intelligent polymers as the responsive matrix. The novel fluorescent chemical sensor can achieve multi-functional cooperation and wider adaptability. In this paper, the self-assembly of thermo-responsive dihydrophilic block copolymers was used to design and prepare water-soluble, temperature-responsive, metal or pH responsive, biocompatible, and water-soluble dihydrophilic block copolymers. A novel metal ion fluorescence chemical sensor based on dihydrophilic block copolymers PEG113-b-PNPAM-co-SHMa65 was prepared. The metal ion recognition unit SHMaa was labeled in the thermosensitive block. It can be used as a multifunctional fluorescent chemical sensor for metal ion and temperature response. It not only can selectively recognize Al3 and Zn2 from many metal ions, but also can distinguish Al3 and Zn2 according to the difference of fluorescence color and fluorescence response time. And has very strong anti-interference ability, excellent water-soluble, high sensitivity and excellent biocompatibility. Once the temperature increases, the temperature sensitive block PNIPAM collapses and the DHBCs self-assemble in aqueous solution, and the metal ion fluorescence response unit SHMa will gather in the hydrophobic nucleus with small polarity, and the quantum yield will increase, and the detection sensitivity will be further improved. In addition, A novel pH fluorescence chemical sensor based on dihydrophilic block copolymers, PEG113-b-PNPAM-co-Rh6GEMa62, was prepared by using PEG113-b-PNNIPAM-co-SHMa-65 for the qualitative and quantitative detection of Al3 or Zn2 in practical biological products. A novel pH fluorescence chemical sensor based on dihydrophilic block copolymers, PEG113-b-PNNIPAM-co-SHMa-co-Rh6GEMa62, was prepared and labeled in thermosensitive block. Basic recognition unit SHMa and acid recognition unit RhSAMa. It can be used as a multifunctional fluorescent chemical sensor for pH response and temperature response. It has the characteristics of wide pH detection range, temperature response, water solubility, biocompatibility, high sensitivity and so on. The detection signal of pH value can be further enhanced when the temperature is higher than its LCST temperature. At the same time, the color difference map and color difference map data 螖 R, 螖 G, 螖 B of the solution at different pH values were obtained by the mobile phone app developed by ourselves. Using the data of 螖 R, 螖 G, 螖 B at pH 1-6 and pH 8-14, respectively, the principal component linear regression quantitative model was established by SPSS20 to quantify the pHs of the solution to be measured. Last Two novel metal ion fluorescence chemical sensors based on bihydrophilic block polymer, PEG113-b-PniPAM-co-BDMaC60 / PEG113-b-PNNIPAM-co-R6GEMa67 were developed by using a quantitative model of principal component linear regression (PCA) for real-time pH detection of ColorExtract. Two novel metal ion fluorescence sensors (PEG113-b-PNPAM-co-R6GEMa67) were prepared, respectively, and labeled Al3 and PNIPAM-co-R6GEMa67 in thermosensitive blocks. Fe3 recognized the elements BDMa and Rh6GE Ma. The mixture can be used as a multifunctional fluorescent chemical sensor for temperature and mixed metal ion response, and has strong anti-interference ability, excellent water solubility, high sensitivity and excellent biocompatibility. In addition, the temperature induced block collapse resulted in the formation of mixed micelles with PNIPAM-co-BDMaand PNNIPAM-co-R6GEMa. which can further improve the sensitivity to Al3 and Fe3 detection. meanwhile In this chapter, a mobile phone app for color recognition is developed to process images to obtain chromatic aberration map and chromatic aberration map data 螖 R, 螖 G, 螖 B before and after solution reaction under different Al3 and Fe 3 mixed ratios. Using SPSS20, the mixed metal ions of Al3 and Fe 3 are established. The principal component linear regression model of proportional quantitative detection was used to verify the model.
【學(xué)位授予單位】：江蘇大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.3

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本文編號(hào)：2033873