本文選題：光子晶體 + “三明治”法�。� 參考：《江南大學(xué)》2016年碩士論文

【摘要】：分子印跡是一種構(gòu)建具有特異性識(shí)別位點(diǎn)的聚合物的有效方法。由于聚合物中形成的納米孔穴在尺寸、空間形狀以及作用點(diǎn)上均與印跡分子相匹配,因此可選擇性識(shí)別被印跡分子。反蛋白石光子晶體由于具有三維有序孔結(jié)構(gòu),與可見(jiàn)光發(fā)生強(qiáng)烈相互作用產(chǎn)生Bragg衍射,呈現(xiàn)出鮮艷的結(jié)構(gòu)顏色。分子印跡凝膠光子晶體(MIPHs)是將反蛋白石光子晶體的三維有序結(jié)構(gòu)與分子印跡聚合物水凝膠相結(jié)合發(fā)展起來(lái)的。MIPHs的體積隨著對(duì)環(huán)境中目標(biāo)分子的特異性識(shí)別吸附發(fā)生變化,引起光子晶體的晶格參數(shù)發(fā)生明顯改變,造成光子晶體的Bragg衍射峰偏移,同時(shí)產(chǎn)生肉眼即可觀察的顏色變化從而實(shí)現(xiàn)對(duì)待測(cè)物的半定量分析。本文制備了兩類(lèi)重要分子L-色氨酸(L-Trp)、葉酸(FA)印跡的凝膠光子晶體,并對(duì)其響應(yīng)性能進(jìn)行了測(cè)試、表征,表明制得的MIPHs具有高選擇性、高靈敏性、信號(hào)自表達(dá)、快速響應(yīng)以及可再生的優(yōu)點(diǎn)。具體研究?jī)?nèi)容與結(jié)果如下:(1)采用經(jīng)典的膠體晶體模板法制備MIPHs,借助兩種前驅(qū)液填充方法“三明治”法和“小角度傾斜滲入”法,均可獲得良好的反蛋白石結(jié)構(gòu)。“三明治”法由于操作更為簡(jiǎn)單方便,為此本文研究的MIPHs采用“三明治”法制得。此外,制備出的MIPHs可與基底剝離,改善了其溶脹-收縮性,并且方便使用。(2)以丙烯酰胺(AM)為功能單體,光引發(fā)聚合制備了L-色氨酸(L-Trp)分子印跡凝膠光子晶體(L-MIPHs),考察了MIPHs對(duì)印跡分子的選擇性。隨著目標(biāo)分子L-Trp濃度增大,分子印跡凝膠光子晶體的Bragg衍射峰紅移83 nm,并能肉眼觀察到其在不同濃度的目標(biāo)分子溶液中呈現(xiàn)不同的顏色。(3)以丙烯酰胺(AM)、馬來(lái)酸酐改性環(huán)糊精(MAH-β-CD)為功能單體,光引發(fā)聚合制備了基于β-CD的L-色氨酸(L-Trp)分子印跡凝膠光子晶體(CD-MIPHs)。采用響應(yīng)面法,利用中心組合實(shí)驗(yàn)設(shè)計(jì),基于有限數(shù)目的實(shí)驗(yàn)評(píng)估了影響MIPHs性能的主要影響因素,并預(yù)測(cè)了最優(yōu)聚合物組成。通過(guò)實(shí)驗(yàn)發(fā)現(xiàn)隨著待測(cè)液中目標(biāo)分子L-Trp濃度不斷增大,MIPHs的Bragg衍射峰紅移126 nm,并對(duì)兩種構(gòu)型的色氨酸表現(xiàn)出良好的手性識(shí)別性能。(4)制備出葉酸(FA)與馬來(lái)酸酐改性環(huán)糊精(MAH-β-CD)的FA-MAH-β-CD包合物,以丙烯酰胺(AM)、FA-MAH-β-CD包合物為功能單體,光引發(fā)聚合制備了基于β-CD的葉酸(FA)分子印跡凝膠光子晶體(F-MIPHs)。制備出的葉酸(FA)分子印跡凝膠光子晶體最低檢測(cè)限可達(dá)到10-12 mol/L。
[Abstract]:Molecular imprinting is an effective method for the construction of polymers with specific identification sites. Because the nano holes formed in the polymer are matched with the imprinted molecules in size, space shape and the point of action, the molecularly imprinted molecules can be identified. The inverse opal photonic crystal has a three-dimensional ordered pore structure and visible light. The strong interaction produces Bragg diffraction, showing a bright structure color. Molecular imprinted gel photonic crystal (MIPHs) is the volume of.MIPHs which combines the three-dimensional ordered structure of the inverse opal photonic crystal and the molecularly imprinted polymer hydrogel, and changes with the specific recognition and adsorption of the target molecules in the environment. The lattice parameters of the photonic crystal change obviously, causing the Bragg diffraction peak shift of the photonic crystal, and producing the color changes that can be observed by the naked eye so as to realize the semi quantitative analysis of the measured objects. This paper prepared two kinds of important molecular L- tryptophan (L-Trp), folic acid (FA) imprinted gel photonic crystal and its response performance. The results are as follows: (1) the preparation of MIPHs by the classical colloidal crystal template method and the "sandwich" method and the "small angle inclined infiltration" method with the help of two kinds of precursor filling methods. The results are as follows: (1) 1) the preparation of the colloidal crystal template method by the classical colloidal crystal template method, and the "sandwich" method and the "small angle inclined infiltration" method by means of the method of filling the precursor. A good inverse opal structure can be obtained. "Sandwich" method is more simple and convenient to operate, for this purpose, the MIPHs method used in this paper is obtained by "sandwich" method. In addition, the prepared MIPHs can be stripped from the substrate, improve its swelling and shrinkage, and be convenient to use. (2) L- is used as a functional monomer and photopolymerization is used to prepare L-. The molecular imprinted gel photonic crystal (L-MIPHs) of tryptophan (L-Trp) was used to investigate the selectivity of MIPHs to the imprinted molecules. As the L-Trp concentration of the target molecule increased, the Bragg diffraction peak of the Molecularly Imprinted Photonic Crystal was red shift 83 nm, and it could be observed by the naked eye to show different colors in the solution of different concentrations of the target molecules. (3) AM A L- tryptophan (L-Trp) Molecularly Imprinted Photonic Crystal (CD-MIPHs) based on L- tryptophan (L-Trp) was prepared by photopolymerization of maleic anhydride modified cyclodextrin (MAH- beta) as a functional monomer. The response surface method was used to evaluate the main influence factors on the performance of MIPHs based on the experimental design of the central combination, and the optimal polymerization was evaluated and the optimal polymerization was predicted. It is found that the Bragg diffraction peak of MIPHs has a red shift of 126 nm with the increasing concentration of the target molecule L-Trp in the solution, and a good chiral recognition performance is shown for the two types of tryptophan. (4) the preparation of the FA-MAH- beta -CD inclusion complex of folic acid (FA) and maleic anhydride modified cyclodextrin (MAH- beta -CD), with acrylamide (AM), FA-M AH- beta -CD inclusion complex is a functional monomer and photopolymerization based on beta -CD (FA) molecular imprinted gel photonic crystal (F-MIPHs) is prepared. The minimum detection limit of the prepared folic acid (FA) molecularly imprinted gel photonic crystal can reach 10-12 mol/L.
【學(xué)位授予單位】：江南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：O631.3;O734
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本文編號(hào)：2018011