本文選題：生物仿生礦化 + 金銀納米簇��； 參考：《曲阜師范大學(xué)》2017年碩士論文

【摘要】：金銀等貴金屬納米簇(NCs)憑借其獨特的物理化學(xué)性質(zhì),被廣泛地應(yīng)用于生物分析、醫(yī)學(xué)成像、催化等領(lǐng)域。本論文分別以谷胱甘肽(GSH)和牛血清白蛋白(BSA)為模板,借助生物仿生礦化途徑,分別合成了銀納米簇(AgNCs)和金-銀納米簇(Au-AgNCs),用以建立了三種簡單、快速、選擇性好、靈敏度高的傳感檢測技術(shù),實現(xiàn)了對植酸(PA)和三聚氰胺(MA)等目標(biāo)物的檢測以及酪氨酸酶(TYR)催化活性的研究。(一)以GSH為模板,在超聲條件下,通過GSH介導(dǎo)銀生物礦化,制備出具有強藍色熒光的AgNCs,進而以熒光AgNCs為探針,借助Fe3+猝滅AgNCs熒光以及引入植酸絡(luò)合Fe3+后可引起其熒光恢復(fù)的現(xiàn)象,設(shè)計了一種熒光“Turn-on”型的傳感檢測技術(shù),實現(xiàn)了谷物中植酸的高靈敏分析(第二章)。結(jié)果表明,與傳統(tǒng)植酸檢測方法比較,本檢測技術(shù)具有操作簡單、選擇性好、靈敏度高等優(yōu)點,可望廣泛應(yīng)用于對谷物等農(nóng)作物的營養(yǎng)成分植酸的分析以及水果保鮮度的評價。(二)以BSA為模板,通過“一鍋式”仿生礦化途徑,制備了強紅色熒光的Au-AgNCs。以Au-AgNCs為熒光探針,利用Au-AgNCs與Hg2+作用引起熒光猝滅,并借助三聚氰胺與Hg2+絡(luò)合形成穩(wěn)定配合物,致使Au-AgNCs熒光恢復(fù)的原理,發(fā)展了一種三聚氰胺熒光傳感分析技術(shù)(第三章)。實驗發(fā)現(xiàn),與單一金或銀納米簇比較,以BSA模板介導(dǎo)的金銀仿生礦化途徑制得的雙金屬型Au-AgNCs,不僅具有顯著增強的熒光,而且能靈敏地響應(yīng)Hg2+和三聚氰胺引起的熒光猝滅與恢復(fù)。由此研制的三聚氰胺熒光檢測法,具有靈敏度高、選擇性好、檢測速度快等優(yōu)點,經(jīng)應(yīng)用于奶制品中三聚氰胺的檢測,獲得了滿意的效果。(三)以BSA模板介導(dǎo)合成的Au-AgNCs為熒光探針,并結(jié)合TYR催化多巴胺氧化反應(yīng)原理,建立了一種快速、靈敏評價TYR活性的熒光檢測方法(第四章)。在溶解氧存在的情況下,多巴胺被TYR催化氧化生成多巴醌,從而引起Au-AgNCs的熒光猝滅,借以實現(xiàn)了對TYR活性的檢測。該方法避免了傳統(tǒng)方法選擇性差、靈敏度低等缺點,為TYR的催化活性評價、以及白癜風(fēng)和白化病等疾病的臨床檢驗診斷,提供了一種新的分析工具。
[Abstract]:Due to its unique physical and chemical properties, gold and silver nanoclusters have been widely used in biological analysis, medical imaging, catalysis and other fields. In this paper, using glutathione (GSH) and bovine serum albumin (BSA) as templates, silver nanoclusters (AgNCs) and gold-silver nanoclusters (Au-AgNCsN) were synthesized by biomimetic mineralization, respectively. The detection of phytate (PAA) and melamine malamine (MAA) and the catalytic activity of tyrosinase (TYR) with high sensitivity were achieved. (1) using GSH as template, silver biomineralization mediated by GSH was used to prepare AgNCswith strong blue fluorescence, and then fluorescent AgNCs were used as probes. Based on the phenomenon that the fluorescence of AgNCs quenched by Fe _ 3 and the fluorescence recovery caused by the introduction of Fe _ 3 complexing with phytic acid, a kind of "Turn-on" type fluorescence sensing technique was designed, which realized the highly sensitive analysis of phytic acid in cereals (Chapter 2). The results show that compared with the traditional phytic acid detection method, this method has the advantages of simple operation, good selectivity, high sensitivity and so on. It is expected to be widely used in the analysis of phytic acid in crops such as cereals and the evaluation of fruit freshness. (2) A strong red fluorescent Au-AgNCswas prepared by "one-pot" bionic mineralization using BSA as template. Using Au-AgNCs as the fluorescence probe, the fluorescence quenching was induced by the interaction of Au-AgNCs with Hg _ 2, and the stable complex was formed by the complexation of melamine with Hg _ 2, which led to the fluorescence recovery of Au-AgNCs. A fluorescence sensing analysis technique for melamine was developed (Chapter 3). Compared with single gold or silver nanoclusters, the bimetallic Au-AgNCsobtained by BSA template mediated gold and silver biomimetic mineralization pathway not only has significantly enhanced fluorescence, but also can sensitively respond to fluorescence quenching and recovery induced by Hg2 and melamine. The developed method has the advantages of high sensitivity, good selectivity and fast detection speed. It has been applied to the detection of melamine in dairy products, and satisfactory results have been obtained. (3) A rapid and sensitive method for the detection of TYR activity was established by using Au-AgNCs synthesized by BSA template as fluorescent probe and combining with the principle of TYR catalytic dopamine oxidation (TYR). In the presence of dissolved oxygen dopamine was oxidized to dopa quinone catalyzed by TYR which resulted in fluorescence quenching of Au-AgNCs and thus the detection of TYR activity was realized. This method avoids the disadvantages of poor selectivity and low sensitivity of traditional methods and provides a new analytical tool for the evaluation of catalytic activity of TYR and the clinical diagnosis of vitiligo and albinism.
【學(xué)位授予單位】：曲阜師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3;TB383.1

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