發(fā)布時間：2018-06-10 15:46

  本文選題：導(dǎo)電聚合物 + 聚苯胺納米線��； 參考：《青島科技大學(xué)》2017年碩士論文

【摘要】：本課題采用電化學(xué)方法,在電極上成功制備了不同的新型導(dǎo)電聚合物納米線復(fù)合材料。然后,基于這些納米線復(fù)合材料構(gòu)建了幾種檢測不同腫瘤標(biāo)志物的電化學(xué)免疫傳感器。通過掃描電子顯微鏡、光電子能譜、電化學(xué)阻抗和微分脈沖伏安法等方法對這些納米線復(fù)合材料的微觀結(jié)構(gòu)、元素組成、電化學(xué)性能和抗污染能力進(jìn)行了表征。我們希望這些新型的生物傳感平臺能夠為實際臨床檢測提供可靠的技術(shù)支持。本課題的主要工作簡述如下:(1)制備了一種新型的基于聚乙二醇/金納米粒子/聚苯胺納米線(PEG/AuNPs/PANI)的電化學(xué)免疫傳感器。該傳感器以聚苯胺納米線(PANI)為基底,繼而修飾上金納米粒子(AuNPs)和聚乙二醇(PEG)。制備的新型PEG/AuNPs/PANI復(fù)合材料,結(jié)合了AuNPs/PANI的優(yōu)良的電導(dǎo)率和PEG良好的抗污染能力,為具有抗污染能力的電化學(xué)免疫傳感器的發(fā)展提供的一個理想的基底。甲胎蛋白(AFP)是眾所周知的肝癌的腫瘤標(biāo)志物,將其作為檢測對象,把它的抗體固定到PEG/AuNPs/PANI復(fù)合材料上,構(gòu)建成一個免標(biāo)記、抗污染的檢測AFP的免疫傳感器。通過使用聚苯胺的氧化還原電流作為傳感信號,結(jié)合PEG/AuNPs良好的生物相容性和PEG優(yōu)異的抗污染能力,使得該傳感器有較寬的線性范圍,最低檢測限達(dá)到0.007 pg mL~(-1)。(2)制備了一種無試劑、免標(biāo)記的用來檢測結(jié)腸直腸癌的重要腫瘤標(biāo)志物癌胚抗原(CEA)的電化學(xué)免疫傳感器。在聚3,4-乙烯二氧噻吩(PEDOT)摻雜離子液體(IL)1-乙基-3-甲基咪唑雙三氟甲磺酰亞胺鹽的多孔復(fù)合材料上,電化學(xué)沉積聚苯胺納米線制備得到網(wǎng)狀復(fù)合材料。該復(fù)合材料的特點是有良好的導(dǎo)電性和比表面積大的多孔結(jié)構(gòu)。在該復(fù)合材料修飾的玻碳電極上,固定癌胚抗原的抗體以檢測癌胚抗原。該免疫傳感器的線性范圍為0.001-10 ng m L~(-1),最低檢測限達(dá)到0.7 pg mL~(-1),同時它還具有良好的穩(wěn)定性和選擇性。(3)在這項工作中,發(fā)展了一種新型的通過剪切特定序列的多肽(peptide)來分析前列腺癌抗原的電化學(xué)方法。首先,在磷酸鹽緩沖溶液中通過電化學(xué)方法合成聚吡咯納米線(Ppy)。然后,在聚吡咯納米線上自組裝金納米粒子(AuNPs)用來固定具有抗污染性能的多肽。制備好的peptide/AuNPs/Ppy新型復(fù)合材料修飾電極,結(jié)合了AuNPs/Ppy優(yōu)良的導(dǎo)電性和peptide良好的抗污染能力,為擁有優(yōu)異抗污染能力的電化學(xué)傳感器的發(fā)展提供的一個極好的基質(zhì)。在前列腺癌抗原的存在下,多肽序列被其特異性的識別并剪切,由此導(dǎo)致的電極阻抗變化可以用于前列腺癌抗原的檢測。在最佳的實驗條件下,該免疫傳感器的線性范圍為0.00001-10 ng mL~(-1)。
[Abstract]:In this paper, different conductive polymer nanowire composites were successfully prepared on the electrode by electrochemical method. Then, based on these nanowire composites, several electrochemical immunosensors were constructed to detect different tumor markers. The microstructure, elemental composition, electrochemical performance and anti-pollution ability of these nanowires were characterized by scanning electron microscopy, photoelectron spectroscopy, electrochemical impedance and differential pulse voltammetry. We hope these new biosensor platforms can provide reliable technical support for practical clinical detection. A novel electrochemical immunosensor based on PEG / au nanoparticles / Polyaniline nanowires PEG / AuNPs / PANIs was prepared. The sensor is based on Polyaniline nanowires (PANI) and then modified with au nanoparticles (AuNPs) and polyethylene glycol (PEGN). The new PEG / AuNPs / pani composite, which combines the excellent conductivity of AuNPs / pani and the good anti-pollution ability of PEG, provides an ideal substrate for the development of electrochemical immunosensors with anti-pollution ability. AFP (alpha-fetoprotein) is a well-known tumor marker of liver cancer. Its antibody was immobilized on PEG / AuNPs / pani composite to construct an anti-AFP immunosensor. By using the redox current of Polyaniline as the sensing signal, combining the good biocompatibility of PEG / AuNPs and the excellent anti-pollution ability of PEG, the sensor has a wide linear range and the minimum detection limit is 0.007 PG / mL ~ (-1). Electrochemical immunosensors for detection of carcinoembryonic antigen (CEA), an important tumor marker in colorectal cancer. The composite was prepared by electrochemical deposition of Polyaniline nanowires on porous composites doped with poly (3-trimethylidene dioxothiophene) (PEDOT) ionic liquid, 1-ethyl-3-methyl imidazolium bis (fluoromethylsulfonimide), and prepared by electrochemical deposition of Polyaniline nanowires. The composite is characterized by good electrical conductivity and large specific surface area porous structure. Antibodies against carcinoembryonic antigen were immobilized on the composite modified glassy carbon electrode to detect carcinoembryonic antigen. The linear range of the immunosensor is 0.001-10 ng / mL ~ (-1), the minimum detection limit is 0.7 PG / mL ~ (-1), and it also has good stability and selectivity. A novel electrochemical method for the analysis of prostate cancer antigens by shearing a specific sequence of peptide peptides has been developed. Firstly, polypyrrole nanowires were synthesized by electrochemical method in phosphate buffer solution. Self-assembled au nanoparticles (AuNPs) were then used to immobilize polypeptides with antifouling properties on polypyrrole nanowires. The new composite modified electrode prepared by Peptide / AuNPs / Ppy combines the excellent conductivity of AuNPs / Ppy and the excellent anti-pollution ability of peptide, which provides an excellent substrate for the development of electrochemical sensors with excellent anti-pollution ability. In the presence of prostate cancer antigen, polypeptide sequences are specifically identified and cut, resulting in changes in electrode impedance can be used to detect prostate cancer antigen. Under the optimum experimental conditions, the linear range of the immunosensor was 0.00001-10 ng / mL ~ (-1).
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 Michael A McNutt;;Elevated serum alpha fetoprotein levels promote pathological progression of hepatocellular carcinoma[J];World Journal of Gastroenterology;2011年41期

2 ;Alpha-fetoprotein stimulated the expression of some oncogenes in human hepatocellular carcinoma Bel 7402 cells[J];World Journal of Gastroenterology;2004年06期

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本文編號：2003747