本文選題：竹材 + 木質(zhì)素��； 參考：《中南林業(yè)科技大學(xué)》2016年碩士論文

【摘要】：我國竹資源豐富,竹纖維具有綠色環(huán)保性、良好吸放濕性、天然抗菌和抗紫外線等特性,具有極大的市場潛力和附加值。竹纖維中木質(zhì)素嚴(yán)重影響了竹纖維產(chǎn)業(yè)化開發(fā)的進(jìn)程,而微生物降解木質(zhì)素是解決這一問題的最有效途徑。本文從降解竹材木質(zhì)素所需的微生物入手,利用微生物繁殖快、易培養(yǎng)、降解能力強的特點,通過不同竹材品種化學(xué)成分的分析、構(gòu)建降解木質(zhì)素的復(fù)合菌系,研究復(fù)合菌系降解竹材工藝,研究結(jié)果為竹原纖維生物降解奠定基礎(chǔ)。主要研究結(jié)果如下：1.不同竹材品種化學(xué)成分的研究通過對不同竹材品種,同種竹材品種不同年齡及不同部位竹材的灰分、木質(zhì)素、綜纖維素及果膠含量等重要化學(xué)成分分析,由于青皮竹或者毛竹的近中部位的竹材的化學(xué)成分中的灰分含量為2.07%、木質(zhì)素含量為22.54%、綜纖維素含量為76.86%、果膠含量為1.11%；毛竹竹材化學(xué)成分中的灰分含量為1.12%、木質(zhì)素含量為27.49%、綜纖維素含量為77.65%、果膠含量為0.92%,因此選用青皮竹或者毛竹的近中部位的竹材作為制漿原料的利用率較高,且能降低生產(chǎn)成本,減輕環(huán)境污染,提高竹材的持續(xù)經(jīng)營和經(jīng)濟(jì)效益等優(yōu)點。2.降解竹材木質(zhì)素高效復(fù)合菌系的構(gòu)建以不同菌株之間拮抗性、不同單菌株對竹材木質(zhì)素降解效果為指標(biāo)初篩選出不存在拮抗性且降解木質(zhì)素能力高的菌株CN-12、HY-31、YY-36、ZYS-19;采用不同復(fù)合菌組合對生物量、竹材失重、竹材木質(zhì)素的降解能力、竹材纖維素的降解能力、對降解選擇性系數(shù)為指標(biāo)復(fù)篩出高降解、高選擇性的竹材木質(zhì)素復(fù)合菌株組合HY-YY-ZYS,其對竹材失重率為27.33%,竹材木質(zhì)素的降解能力為38.19%、竹材纖維素的降解能力為10.49%、降解選擇性系數(shù)為3.64：且經(jīng)過復(fù)合菌組合的穩(wěn)定性研究,表明該復(fù)合菌株組合木質(zhì)素降解能力穩(wěn)定,平均降解率達(dá)到38.54%,比降解能力最強的單菌株(ZYS-19)的降解率提高了8.78%。3.復(fù)合菌降解竹材木質(zhì)素固體發(fā)酵工藝條件研究以降解率和選擇系數(shù)為主要指標(biāo),采用單因素優(yōu)化方法結(jié)合均勻設(shè)計試驗,對復(fù)合菌株組合(BY-YY-ZYS)固體發(fā)酵降解竹材木質(zhì)素培養(yǎng)基條件進(jìn)行了優(yōu)化：竹粉(40目)15.0g,以1：3的料液比加入合成培養(yǎng)液,酒石酸銨0.2 g/L,葡萄糖14g/L, Ca2+0.8mmol/L,Mn2+ 0.6mmol/L時,降解率為46.29%,選擇系數(shù)為10.72。以降解率和選擇系數(shù)為主要指標(biāo),采用正交設(shè)計試驗,對復(fù)合菌株組合(HY-YY-ZYS)固體發(fā)酵降解竹材木質(zhì)素工藝條件進(jìn)行了優(yōu)化,結(jié)果表明：接種量為6%、含水量為50%、溫度32℃、pH 5.0,在此條件下固體發(fā)酵25d時,竹材木質(zhì)素降解率為60.29%,選擇系數(shù)為12.79,降解率增加了21.75%,選擇系數(shù)增加了9.15,降解時間縮短5d,竹材木質(zhì)素降解率、選擇系數(shù),生產(chǎn)效率均得到了提高,且降低了生產(chǎn)成本。
[Abstract]:China is rich in bamboo resources, bamboo fiber has green environmental protection, good moisture absorption and desorption, natural antibacterial and anti-ultraviolet characteristics, with great market potential and added value. Lignin in bamboo fiber seriously affects the development process of bamboo fiber industrialization, and microbial degradation of lignin is the most effective way to solve this problem. Based on the microbes needed to degrade the lignin of bamboo wood, by using the characteristics of rapid propagation, easy culture and strong degradation ability, the complex strain of lignin degradation was constructed by analyzing the chemical composition of different bamboo varieties. The process of bamboo degradation by composite bacteria was studied. The results laid a foundation for the biodegradation of bamboo fiber. The main results are as follows: 1. The chemical constituents of different bamboo varieties were analyzed by analyzing the ash content, lignin, holocellulose and pectin content of different bamboo varieties, the same bamboo varieties, different ages and different parts of bamboo. Because the chemical composition of bamboo is 2.07, lignin is 22.54, holocellulose is 76.86, pectin is 1.11, bamboo ash is 1.12 and lignin is 1.12. The content is 27.49, the content of holocellulose is 77.65, the content of pectin is 0.92k.Therefore, the utilization rate of bamboo in the near and middle part of green bamboo or Phyllostachys pubescens as raw material for pulping is higher. And it can reduce the production cost, reduce the environmental pollution, improve the sustainable management of bamboo and economic benefits. 2. 2. The biodegradation of bamboo lignin efficient complex strain was constructed to antagonize between different strains. The effect of different strains on lignin degradation of bamboo wood was studied. The strain CN-12HY-31, YY-36, ZYS-19, which had no antagonism and high ability of lignin degradation, was selected initially, and the biodegradation ability of biomass, bamboo weight loss and bamboo lignin was studied by using different combinations of bacteria. The degradation ability of bamboo cellulose, the selectivity coefficient of degradation was the index of high degradation, The weight loss rate of HY-YY-ZYS was 27.33, the degradation ability of bamboo lignin was 38.19, the degradation ability of bamboo cellulose was 10.499.The degradation selectivity coefficient was 3.64: and the stability of compound bacteria combination was studied. The results showed that the lignin degradation ability of the compound strain was stable, the average degradation rate was 38.54, and the degradation rate of ZYS-19, the single strain with the strongest degradation ability, was 8.78.3. Study on the technological conditions for the degradation of lignin from Bamboo Wood by Composite bacteria the degradation rate and the selection coefficient were taken as the main indexes and the single factor optimization method was used to combine the uniform design test. The conditions of solid fermentation medium for the degradation of bamboo lignin by compound strain BY-YY-ZYS were optimized. The results showed that the degradation rate of bamboo lignin was 46.29g / L and the selectivity coefficient was 10.72when the mixture was added to the synthetic medium at 1:3, when ammonium tartrate was 0.2 g / L, glucose was 14 g / L, Ca2 was 0.8 mmol / L ~ (2) mn _ (2), the degradation rate was 46.29g 路L ~ (-1) and the selection coefficient was 10.72 g 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). With the degradation rate and selection coefficient as the main indexes, the process conditions for the degradation of bamboo lignin by solid fermentation of compound strain combination HY-YY-ZYS were optimized by orthogonal design. The results showed that the inoculation amount was 6, the water content was 50, the temperature was 32 鈩，

本文編號：1981330