發(fā)布時(shí)間：2018-05-31 16:12

  本文選題：熒光銅納米簇 + 二氧化錳納米片�。� 參考：《信陽(yáng)師范學(xué)院》2017年碩士論文

【摘要】：熒光金屬納米團(tuán)簇具有尺寸小、易于合成、較強(qiáng)的抗光漂白性、良好的生物相容性等優(yōu)點(diǎn),是一種新型的納米材料。近年來(lái),由于熒光銅納米簇具有成本低、水溶性好、光學(xué)性能穩(wěn)定、可調(diào)的熒光發(fā)射等特點(diǎn),在生物分析、生物成像、醫(yī)療應(yīng)用、環(huán)境監(jiān)測(cè)等領(lǐng)域引起了廣泛的研究興趣。本文利用Poly(T)DNA和牛血清白蛋白作為合成模板,合成了不同熒光發(fā)射波長(zhǎng)的熒光銅納米簇,并以此作為熒光信號(hào)探針,檢測(cè)生物小分子和巰基化合物。此外,蛋白質(zhì)為模板的熒光銅納米簇可以與二氧化錳納米片形成復(fù)合物,開(kāi)發(fā)了一種新型、靈敏、無(wú)標(biāo)記的傳感新方法,用于蛋白質(zhì)的檢測(cè)。主要研究?jī)?nèi)容如下:(1)利用曲酸阻止Poly(T)DNA模板的熒光銅納米簇的形成,構(gòu)建了一種簡(jiǎn)單,快速,非標(biāo)記型的熒光傳感新方法并用于曲酸的靈敏檢測(cè)。熒光銅納米簇以Poly(T)DNA作為模板,以抗壞血酸為還原劑,在室溫下5分鐘內(nèi)合成。當(dāng)目標(biāo)曲酸存在時(shí),由于Cu~(2+)和曲酸形成穩(wěn)定的絡(luò)合物,有效地阻止了Poly(T)DNA模板熒光銅納米簇形成,從而導(dǎo)致體系熒光信號(hào)減弱。在優(yōu)化的條件下,該方法對(duì)曲酸檢測(cè)的線性范圍為0.1~300μmol/L,檢出限為10 nmol/L。同時(shí),該方法用于食品樣品中曲酸含量的測(cè)定,RSD范圍在2.7%~4.2%,表明該方法是可行的。(2)基于阻礙半胱氨酸(Cys)猝滅Poly(T)DNA模板的熒光銅納米簇的熒光,發(fā)展了一種快速、靈敏、無(wú)標(biāo)記的熒光生物傳感策略用于Hg~(2+)的檢測(cè)。在Cys存在的情況下,Cys與銅納米簇之間形成穩(wěn)定的Cu-S鍵,從而有效地猝滅銅納米簇的熒光信號(hào)。當(dāng)體系加入Hg~(2+)后,Cys與Hg~(2+)形成了更穩(wěn)定的Hg-S鍵,導(dǎo)致Cys遠(yuǎn)離了熒光銅納米簇的表面,體系的熒光強(qiáng)度得到恢復(fù)。該方法實(shí)現(xiàn)了對(duì)目標(biāo)Hg~(2+)的靈敏檢測(cè),線性范圍為0.5~100 nmol/L,檢出限為0.1 nmol/L,用于實(shí)際樣品檢測(cè),RSD在2.36%~3.12%,表明該方法可用于實(shí)際樣品的檢測(cè)。(3)基于MnO_2納米片-熒光銅納米簇復(fù)合物,建立了一種新型的熒光傳感策略用于谷胱甘肽的高靈敏、高選擇性檢測(cè)。熒光銅納米簇以牛血清白蛋白為模板,通過(guò)簡(jiǎn)便的一步化學(xué)還原法制得。當(dāng)MnO_2納米片-熒光銅納米簇復(fù)合物形成時(shí),銅納米簇的熒光信號(hào)能夠通過(guò)熒光能量共振轉(zhuǎn)移被MnO_2納米片有效地猝滅。當(dāng)加入目標(biāo)谷胱甘肽時(shí),MnO_2納米片能夠被還原生成Mn2+,從而破壞了MnO_2納米片,使MnO_2納米片引起的熒光猝滅效應(yīng)得到抑制,最終體系的熒光信號(hào)得到恢復(fù)。該方法的線性檢測(cè)范圍為0~300μmol/L,檢出限為100 nmol/L,用于實(shí)際樣品檢測(cè),RSD范圍3.3%~4.3%,同時(shí)具有較好的選擇性。結(jié)果表明,該方法具有簡(jiǎn)單,快速,成本低,易于制備等優(yōu)點(diǎn)。(4)利用MnO_2納米片-熒光銅納米簇復(fù)合物作為熒光探針,基于H_2O_2誘導(dǎo)破壞猝滅劑MnO_2納米片,開(kāi)發(fā)了一種靈敏的熒光生物傳感新方法用于葡萄糖的測(cè)定。當(dāng)H_2O_2存在時(shí),MnO_2被還原生成Mn2+,銅納米簇從MnO_2納米片表面釋放出來(lái),體系的熒光信號(hào)得到恢復(fù)。另一方面,葡萄糖在葡萄糖氧化酶存在條件下,被催化氧化生成葡萄糖酸和H_2O_2,因此,通過(guò)酶催化產(chǎn)生的H_2O_2導(dǎo)致的熒光信號(hào),可以間接地檢測(cè)葡萄糖的濃度。該方法的線性檢測(cè)范圍為1~200μmol/L,檢出限為100 nmol/L。同時(shí),RSD在2.9%~4.2%,表明該方法可用于人血清樣品中葡萄糖含量的測(cè)定。
[Abstract]:The fluorescent metal nanoclusters have the advantages of small size, easy synthesis, strong anti photobleaching, good biocompatibility and so on. It is a new kind of nano material. In recent years, the fluorescent copper nanoclusters have the characteristics of low cost, good water solubility, stable optical properties, adjustable fluorescein and so on, in biological analysis, bioimaging, medical applications, and so on. In this paper, Poly (T) DNA and bovine serum albumin (BSA) are used as a synthetic template to synthesize fluorescent copper nanoclusters with different fluorescence emission wavelengths, which are used as a fluorescent probe to detect small biological molecules and sulfhydryl compounds. In addition, the fluorescent copper nanoclusters of protein as a template can be used as a template. A new, sensitive and unmarked new sensing method was developed for the detection of protein. The main contents of this study are as follows: (1) a simple, fast, unlabeled new fluorescence sensing method was constructed and used in the spirit of kojic acid by using kojic acid to prevent the formation of Poly (T) DNA template. Poly (T) DNA as a template and ascorbic acid as a reductant and synthesized in 5 minutes at room temperature. When target ascorbic acid exists, Cu~ (2+) and kojic acid form a stable complex, which effectively prevents the formation of Poly (T) DNA template fluorescent copper nanoclusters, resulting in a weakening of the fluorescence signal of the system. At the same time, the linear range of the method is 0.1~300 mu mol/L and the detection limit is 10 nmol/L.. The method is used to determine the content of kojic acid in food samples. The RSD range is 2.7%~4.2%, indicating that the method is feasible. (2) a rapid development is developed based on the fluorescence of the fluorescent copper nanoclusters that hinder the quenching of Poly (T) DNA template with cysteine (Cys). A sensitive, unmarked fluorescence biosensor strategy is used for the detection of Hg~ (2+). In the presence of Cys, a stable Cu-S bond between Cys and copper nanoclusters is formed, which effectively quenches the fluorescence signals of the copper nanoclusters. When Hg~ (2+) is added to the system, Cys and Hg~ (2+) form a more stable Hg-S bond, which leads to Cys away from the table of the fluorescent copper nanoclusters. The fluorescence intensity of the system is restored. The method realizes the sensitive detection of the target Hg~ (2+). The linear range is 0.5~100 nmol/L, the detection limit is 0.1 nmol/L. The method is used for the actual sample detection and RSD in 2.36%~3.12%. It shows that the method can be used for the detection of actual samples. (3) a kind of MnO_2 nanoscale copper nanocluster complex is established. The new fluorescence sensing strategy is used for high sensitivity and high selectivity of glutathione. The fluorescent copper nanocluster is prepared by a simple one-step chemical reduction method using bovine serum albumin as a template. When the MnO_2 nanoscale copper nanocluster complex is formed, the fluorescence signal of the copper nanocluster can be transferred by MnO_2 to the fluorescence energy resonance. MnO_2 nanoscale can be reduced to Mn2+ when the target glutathione is added. The MnO_2 nanoscale is destroyed and the fluorescence quenching effect caused by the MnO_2 nanoscale is suppressed. The fluorescence signal of the final system is restored. The linear detection of this method is 0~300 micron mol/L, the detection limit is 100 nmol/L, used for real time. The results show that the method has the advantages of simple, rapid, low cost, easy to prepare and so on. (4) a sensitive fluorescent biological transmission is developed by using MnO_2 nanoscale fluorescent copper nanocluster complex as a fluorescent probe and H_2O_2 induced sudden destruction agent MnO_2 nanoscale based on H_2O_2. A new sense method is used for the determination of glucose. When H_2O_2 exists, MnO_2 is reduced to Mn2+, and the copper nanocluster is released from the surface of MnO_2 nanoscale. The fluorescence signal of the system is restored. On the other hand, glucose is catalyzed to produce gluconic acid and H_2O_2 under the condition of glucose oxidase. Therefore, the H_2 produced by enzyme catalyzed. The fluorescence signal caused by O_2 can be used to detect the concentration of glucose indirectly. The linear detection range of this method is 1~200 mol/L, the detection limit is 100 nmol/L., and RSD is 2.9%~4.2%. It shows that the method can be used for the determination of glucose content in human serum samples.
【學(xué)位授予單位】：信陽(yáng)師范學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 ;Copper nanoclusters:Synthesis,characterization and properties[J];Chinese Science Bulletin;2012年01期

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本文編號(hào)：1960484