本文選題：羅丹明 + 熒光探針��； 參考：《海南師范大學(xué)》2017年碩士論文

【摘要】：當(dāng)今,環(huán)境中的重金屬污染問題已日益加重,一些重金屬在人和動(dòng)物體內(nèi)扮演了很重要的角色。在眾多重金屬離子檢測(cè)方法中,熒光探針法具有靈敏度高、特異性強(qiáng)、方便快捷等特點(diǎn)。羅丹明類熒光染料具有毒性小、水溶性好、光量子產(chǎn)率高等優(yōu)點(diǎn)。因此研究者廣泛的關(guān)注了羅丹明類熒光分子探針。本文概述了羅丹明類熒光分子探針的研究進(jìn)展,在此基礎(chǔ)上,以羅丹明衍生物作為母體,主要設(shè)計(jì)合成了3種熒光探針。具體內(nèi)容如下:(1)一種基于丹酰氯與羅丹明B的新型熒光探針�？梢酝ㄟ^顯示獨(dú)特的熒光和吸收特性從其他常見金屬離子中選擇性識(shí)別Hg~(2+)和Cu~(2+)。在MeCN/HEPES緩沖溶液中,探針對(duì)Hg~(2+)具有比例熒光響應(yīng),這歸因于從丹�；介_環(huán)的羅丹明B的熒光共振能量轉(zhuǎn)移,但當(dāng)Cu~(2+)的存時(shí)候能引起熒光猝滅,其他金屬離子對(duì)該探針沒有影響。(2)通過亞胺鍵連接羅丹明6G酰肼與N-甲基靛紅合成了一個(gè)新的熒光探針,可以選擇性識(shí)別和傳感Pb~(2+)、Hg~(2+)和Cd~(2+)。在乙醇/HEPES緩沖溶液中,加入Cd~(2+)導(dǎo)致黃綠熒光,而Pb~(2+)或Hg~(2+)的存在產(chǎn)生橙色熒光。此外,探針顯示對(duì)Pb~(2+)的不可逆熒光響應(yīng)和對(duì)Hg~(2+)和Cd~(2+)的可逆熒光響應(yīng)。(3)用乙二胺連接羅丹明B和7-二乙胺基-3-醛基香豆素合成了一個(gè)熒光共振能量轉(zhuǎn)移系統(tǒng)RC,合成方法簡(jiǎn)便、快速,產(chǎn)物經(jīng)核磁共振、質(zhì)譜和X-射線單晶衍射確認(rèn)。
[Abstract]:Nowadays, the pollution of heavy metals in the environment is becoming more and more serious, and some heavy metals play an important role in human and animal. Among many heavy metal ion detection methods, the fluorescence probe method has the characteristics of high sensitivity, specificity, convenience and so on. Rhodamine fluorescent dyes have the advantages of low toxicity, good water solubility and high light quantum yield. So researchers have focused extensively on Rhodamine fluorescent probes. In this paper, the research progress of Rhodamine fluorescent molecular probes is summarized. On the basis of this, three kinds of fluorescent probes are designed and synthesized with Rhodamine derivatives as the parent. A novel fluorescent probe based on Danyl chloride and rhodamine B. Hg~(2 and Cu~(2 can be selectively identified from other common metal ions by displaying unique fluorescence and absorption characteristics. In MeCN/HEPES buffer solution, the probe has a proportional fluorescence response to Hg~(2, which is attributed to the fluorescence resonance energy transfer from the Danyl group to the open ring Rhodamine B, but it can cause fluorescence quenching when the Cu~(2) exists. Other metal ions had no effect on the probe. 2) A new fluorescent probe was synthesized by linking Rhodamine 6G hydrazide with N-methylindirubin by imine bond, which can selectively recognize and sensing the Pb~(2 and Cd~(2. In ethanol / HEPES buffer solution, the addition of Cd~(2) leads to yellowish green fluorescence, while the presence of Pb~(2) or Hg~(2) produces orange fluorescence. In addition, The probe showed irreversible fluorescence response to Pb~(2 and reversible fluorescence response to Hg~(2 and Cd~(2).) A fluorescence resonance energy transfer system was synthesized by using ethylenediamine to link rhodamine B and 7-diethylamino -3-aldehyde coumarin. The method is simple and convenient. The product was confirmed by NMR, MS and X-ray single crystal diffraction.
【學(xué)位授予單位】：海南師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 Xiao-Bo Li;Zhi-Gang Niu;Ling-Ling Chang;Men-Xia Chen;En-Ju Wang;;Quinoline-based colorimetric chemosensor for Cu~(2+): Cu~(2+)-induced deprotonation leading to color change[J];Chinese Chemical Letters;2014年01期

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本文編號(hào)：1958171