本文選題：多肽組 + 超濾　； 參考：《分析化學(xué)》2017年02期

【摘要】：漏出性胸腔積液中的多肽和蛋白質(zhì)等生物分子直接或間接地與機(jī)體特定的生理、病理狀態(tài)相關(guān),反映了肺部或者全身其它部位疾病的信息。本研究利用超濾法將漏出性胸腔積液中的多肽組分進(jìn)行分離,經(jīng)脫鹽富集后,進(jìn)行納升液相色譜-串聯(lián)質(zhì)譜分析。結(jié)果表明,在漏出性胸腔積液中共鑒定到來源于52種蛋白的314條多肽,超過一半的肽段來源于纖維蛋白原,并且許多肽段具有階梯序列的特征。此外,在來源于膠原蛋白和纖維蛋白原的多肽中還發(fā)現(xiàn)了大量的脯氨酸氧化修飾�；虮倔w論富集分析顯示,漏出性胸腔積液多肽組分所屬蛋白均具有胞外分泌的屬性。本研究給出了漏出性胸腔積液中多肽組的序列、等電點(diǎn)、分子量、翻譯后修飾等理化參數(shù)的分布特征,為進(jìn)一步尋找肺部疾病相關(guān)的多肽標(biāo)志物提供了可借鑒的參考數(shù)據(jù)和分析方法。
[Abstract]:The biological molecules such as peptides and proteins in the effusion of effusion are directly or indirectly related to the specific physiological and pathological state of the body and reflect the information of diseases in the lungs or other parts of the body. In this study, the polypeptide components in effusion were separated by ultrafiltration method, and then were analyzed by liquid chromatography-tandem mass spectrometry after desalination and enrichment. The results showed that a total of 314 peptides from 52 proteins were identified in effusion and more than half of the peptides were derived from fibrinogen and many of them had the characteristics of ladder sequence. In addition, a large number of proline oxidative modifications were found in polypeptides derived from collagen and fibrinogen. Gene ontological enrichment analysis showed that the proteins of the polypeptide components in the effusion of effusion had the properties of extracellular secretion. The distribution characteristics of polypeptide group sequence, isoelectric point, molecular weight, post-translational modification and other physical and chemical parameters in leaky pleural effusion were given in this study. It provides reference data and analytical methods for further searching for polypeptide markers related to lung diseases.
【作者單位】： 吉林大學(xué)第二醫(yī)院;吉林省腫瘤醫(yī)院;吉林大學(xué)中日聯(lián)誼醫(yī)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(Nos.81572082,81472030) 吉林省科技廳項(xiàng)目(Nos.20150101122JC,20150204001YY,20140311092YY)資助~~
【分類號】：R561.3;O657.63

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1 朱洪斌;多指標(biāo)聯(lián)合檢測鑒別滲出與漏出性胸腔積液的研究[D];安徽醫(yī)科大學(xué);2011年

2 張家強(qiáng);特異性蛋白質(zhì)在結(jié)核性、惡性及漏出性胸腔積液中的表達(dá)差異及其鑒別診斷意義研究[D];昆明醫(yī)科大學(xué);2014年

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本文編號：1937107