本文選題：和頻光譜 + 手性　； 參考：《中國科學技術大學》2017年碩士論文

【摘要】：在生命體內,特定手性的分子才能發(fā)揮特定的功能,以維持正常的生命活動。研究生物分子在生物膜界面的手性問題對于正確理解某些疾病的發(fā)病機理非常重要。因此加深對生物膜界面的手性生物分子的研究有助于理解相關疾病的發(fā)生機理,也為新型藥物的研究、合成提供指導。然而生物膜界面的手性分子的研究很復雜,目前有效、實時、原位、免標記表征的研究手段很少。針對這一現狀,本文利用界面敏感的、手性和非手性偏振結合的和頻光譜測量技術,以兩種手性生物分子,即膽固醇和hIAPP蛋白為模型,系統(tǒng)研究其在生物膜界面的組裝和運輸行為,以及蛋白在生物膜界面的結構轉變過程。通過研究膽固醇分子在電中性的PC類磷脂膜和負電荷PG類磷脂膜中的翻轉運輸行為以及組裝方式,給出膽固醇分子在此體系中的翻轉運輸時間尺度以及組裝方式,得出膽固醇在磷脂雙層膜中是否翻轉主要取決于磷脂是否翻轉的結論。同時在PG類磷脂膜中引入鹽離子,再次證明了膽固醇是否翻轉取決于磷脂分子是否翻轉的結論。在研究hIAPP蛋白在磷脂雙層膜中的結構演變動力學中,通過系統(tǒng)研究不同相態(tài)的磷脂膜以及抑制劑對hIAPP蛋白在生物膜中結構變化的影響,結果發(fā)現hIAPP在生物膜界面上會發(fā)生聚集,而酰胺Ⅱ譜帶的出現與聚集的程度有關。
[Abstract]:In life, specific chiral molecules can perform specific functions to maintain normal life activities. It is very important to study the chirality of biomolecules at the biomembrane interface in order to understand the pathogenesis of some diseases correctly. Therefore, the further study of chiral biomolecules at the biofilm interface is helpful to understand the pathogenesis of related diseases and provide guidance for the study and synthesis of novel drugs. However, the study of chiral molecules at biofilm interface is very complicated. In this paper, two chiral biomolecules, cholesterol and hIAPP protein, were used as models of chiral, chiral and non-chiral polarization-binding sum-frequency spectroscopy. The assembly and transport behavior at the biofilm interface and the structural transformation of proteins at the biofilm interface were systematically studied. The flipping transport behavior and assembly mode of cholesterol molecules in electroneutral phospholipid membrane (PC) and negatively charged phospholipid (PG) membrane were studied. It is concluded that whether cholesterol flips in the bilayer membrane of phospholipid mainly depends on whether the phospholipid is flipped. At the same time, salt ions were introduced into PG phospholipid membrane, which proved that whether cholesterol flips depends on whether phospholipid molecules flip or not. In the study of the structural evolution kinetics of hIAPP protein in phospholipid bilayer membrane, the effects of different phase phospholipid membrane and inhibitor on the structural change of hIAPP protein in biofilm were systematically studied. The results showed that hIAPP aggregates at the interface of biofilm. The appearance of amide-II band is related to the degree of aggregation.
【學位授予單位】：中國科學技術大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.3;O647.1

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本文編號：1931165