本文選題：竹茹 + 多糖　； 參考：《中國(guó)食品學(xué)報(bào)》2017年07期

【摘要】：目的:研究竹茹多糖(BSP)的結(jié)構(gòu)特性及體外免疫調(diào)節(jié)活性。方法:利用甲基化分析及核磁共振波譜法解析BSP的化學(xué)結(jié)構(gòu);采用小鼠脾臟淋巴細(xì)胞轉(zhuǎn)化增殖試驗(yàn),RAW264.7巨噬細(xì)胞吞噬中性紅以及分泌NO試驗(yàn)評(píng)價(jià)BSP的體外免疫調(diào)節(jié)活性。結(jié)果:BSP為乙酰化的阿拉伯糖基4-O-甲基葡萄糖醛酸木聚糖,其結(jié)構(gòu)為:以β-1,4-D-木糖為骨架,在木糖基的C-2位上連接有乙�；�4-O-甲基-α-D-葡萄糖醛酸,C-3位上連接有乙�；颚�-L-阿拉伯糖;BSP在10~100μg/m L范圍內(nèi)能促進(jìn)刀豆蛋白或脂多糖誘導(dǎo)的小鼠脾臟淋巴細(xì)胞增殖;BSP在10~1 000μg/m L范圍可顯著提高RAW 264.7巨噬細(xì)胞吞噬中性紅及生成NO的能力,并呈明顯的劑量依賴性。結(jié)論:原料來源豐富的竹茹多糖具有作為天然免疫調(diào)節(jié)劑的潛力。
[Abstract]:Objective: to study the structure and immunomodulatory activity of BSPs. Methods: the chemical structure of BSP was analyzed by methylation analysis and nuclear magnetic resonance spectroscopy (NMR), the phagocytosis of neutral red by RAW264.7 macrophages and the in vitro immunomodulatory activity of BSP were evaluated by mouse spleen lymphocyte transformation and proliferation test (RAW264.7). Results: BSP was acetylated arabinyl 4-Omethylglucuronic acid xylan, and its structure was as follows: 尾 -1N 4-D-xylose was used as skeleton. Ligation of acetyl or 4-Omethyl- 偽 -Dglucuronic acid C-3 on C-2 site of xylose group with acetyl group or 偽 -L-arabinose BSP on C-3 site can promote proliferation of spleen lymphocytes induced by concanavalin or lipopolysaccharide in 10 ~ 100 渭 g / m L range. The ability of RAW 264.7 macrophages to phagocytosis of neutrophilic red and to produce no was significantly increased in the range of 10 ~ 1 000 渭 g 路mL ~ (-1) ~ (-1) 渭 g 路mL ~ (-1). And in a dose-dependent manner. Conclusion: the polysaccharide with abundant raw materials has the potential as a natural immunomodulator.
【作者單位】： 浙江大學(xué)生物系統(tǒng)工程與食品科學(xué)學(xué)院浙江省農(nóng)產(chǎn)品加工技術(shù)研究重點(diǎn)實(shí)驗(yàn)室浙江省食品加工技術(shù)與裝備工程中心;
【分類號(hào)】：O629.12
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本文編號(hào)：1921741