本文選題：熒光相關光譜 + 熒光量子點��； 參考：《青島大學》2017年碩士論文

【摘要】：由于測量具有很高的靈敏度、測量時不破壞研究體系的平衡狀態(tài)以及可以進行實時監(jiān)測等優(yōu)點,熒光相關光譜(FCS)技術在化學、生命科學等領域得到越來越廣泛的應用,特別是在生物化學領域內對原位測量顯示出獨有的優(yōu)勢。量子點由于特殊的尺寸和優(yōu)異的物理化學性能,在生物化學領域吸引了越來越多的關注。當量子點被引入到蛋白質或細胞中后,它們可以被許多的蛋白質包覆而形成一個蛋白質外殼,稱為“蛋白質冕”。這樣,被包被了蛋白質的量子點就有了更好的生物相容性,有利于量子點的生物應用和毒性研究。因此本文基于熒光相關光譜法,研究了典型的熒光納米材料─CdSe/ZnS水溶性羧基量子點,對生物大分子的相互結合的溫度和pH的依賴性,并進行了量子點與蛋白質結合的熱力學分析;并且通過圓二色譜法和模擬計算得到了CdSe/ZnS水溶性羧基量子點與蛋白質之間可能的結合位點和可能的結合方式�；跓晒庀嚓P光譜技術探究了原位條件下不同基團包覆的CdSe/ZnS水溶性點與血漿和血清的結合程度,實現(xiàn)了在緩沖液以及純血清和純血液的原位環(huán)境下對FCS的應用。探究了金和銀納米顆粒對不同濃度BSA包覆的不同厚度的CdSe/ZnS水溶性羧基量子點的熒光壽命的影響,即相對作用距離對CdSe/ZnS水溶性羧基量子點的熒光壽命的影響。結果表明隨著BSA對CdSe/ZnS水溶性羧基量子點的包覆厚度增大,CdSe/ZnS水溶性羧基量子點的熒光壽命都向短波長方向移動;短壽命物種的比例從14.75%增加到28.93%,長壽命物種的比例從85.25%減少到71.07%。該研究課題對于量子點的生物原位應用和毒性研究有重要意義。
[Abstract]:Because of the advantages of high sensitivity, no damage to the equilibrium state of the research system and the ability to monitor the system in real time, the fluorescence correlation spectroscopy (FCSs) technology has been widely used in the fields of chemistry, life science and so on. In particular, in the field of biochemistry, in situ measurement shows unique advantages. Quantum dots have attracted more and more attention in the field of biochemistry because of their special size and excellent physical and chemical properties. When quantum dots are introduced into proteins or cells, they can be coated with many proteins to form a protein shell called a "protein corona". In this way, the quantum dots coated by protein have better biocompatibility, which is beneficial to the biological application and toxicity study of quantum dots. Therefore, based on fluorescence correlation spectroscopy, the dependence of typical fluorescent nanomaterials, CdSe/ZnS water-soluble carboxyl quantum dots, on the binding temperature and pH of biomolecules is studied. The possible binding sites and possible binding modes between CdSe/ZnS water-soluble carboxyl quantum dots and proteins were obtained by circular dichroism and simulation. Based on fluorescence correlation spectroscopy, the binding degree of water-soluble sites of CdSe/ZnS coated with different groups to plasma and serum was investigated in situ, and the application of FCS in buffer solution, in situ environment of pure serum and pure blood was realized. The effect of gold and silver nanoparticles on the fluorescence lifetime of CdSe/ZnS water-soluble carboxyl quantum dots coated with different concentrations of BSA was investigated, that is, the influence of the relative distance of action on the fluorescence lifetime of CdSe/ZnS water-soluble carboxyl quantum dots. The results show that the fluorescence lifetime of BSA / ZnS water-soluble carboxyl quantum dots moves towards short wavelength with the increase of the coating thickness of CdSe/ZnS water-soluble carboxyl quantum dots, the proportion of short-lived species increases from 14.75% to 28.93%, and the proportion of long-lived species decreases from 85.25% to 71.07%. This research is of great significance for the biological in situ application and toxicity study of quantum dots.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.3

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