本文選題：閉合型雙極化電極 + 多巴胺��； 參考：《湘潭大學(xué)》2016年碩士論文

【摘要】：雙極化電化學(xué)是以雙極化電極(BPE)為工作電極,電極的兩端分別發(fā)生氧化或還原反應(yīng)的一種電化學(xué)研究方法,它包括開放型雙極化體系和閉合型雙極化體系兩種類型。本論文主要研究的是閉合型雙極化體系,基于閉合型雙極化原理,我們?yōu)槭竽X中一些神經(jīng)遞質(zhì)或活性分子(如多巴胺、抗壞血酸)的電化學(xué)檢測提供了一種新型的有效檢測方法。具體工作概括如下:(1)我們首先研究了閉合型雙極化體系中電極尺寸和電極兩端物質(zhì)的濃度對半波電位的影響,然后選擇一個最合適的K_3Fe(CN)_6濃度和電極尺寸制備了K_3Fe(CN)_6溶液填充的圓柱形碳纖維電極(CFEs),用于原位檢測鼠腦中的多巴胺(DA)。并比較了該電極與傳統(tǒng)的填充KCl或Ru(NH3)6Cl3溶液的電極穩(wěn)定性,靈敏度和半波電位的大小,最后證明我們所制備的電極對活體中兒茶酚胺的檢測具有高穩(wěn)定性、高靈敏度和對動物的低損害性等優(yōu)點。(2)我們對傳統(tǒng)意義上的閉合型雙極化電極裝置進行了一定的改裝,即撤去外界所施加的電壓,在電極兩端分別加入可以發(fā)生氧化或還原反應(yīng)的電化學(xué)物質(zhì),利用電極兩端自發(fā)地發(fā)生氧化或還原反應(yīng)產(chǎn)生的電勢差,將該體系改裝成一個可自行發(fā)電的原電池。電壓和功率經(jīng)兩根理想型非極化電極(閉合型雙極化體系中的驅(qū)動電極)進行輸出。在此設(shè)計下,我們研究了H_2-O_2,K_4Fe(CN)_6-O_2,K_3Fe(CN)_6-Fc(COOH)2,K_3Fe(CN)_6-DA四種電池的工作性能,發(fā)現(xiàn)功率和電壓均有較好地輸出。(3)基于改裝后的原電池原理的閉合型雙極化體系,我們制備了一種檢測活體中抗壞血酸釋放的微型生物傳感器。即在碳纖維電極內(nèi)填充含有支持電解質(zhì)的一定濃度的K_3Fe(CN)_6溶液,并在電極表面修飾多壁碳納米管,將其作為工作電極用于活體中抗壞血酸的原位檢測。我們對該基于原電池原理所制備的生物傳感器性能進行了研究,發(fā)現(xiàn)利用此方法可以實現(xiàn)對鼠腦中抗壞血酸的高穩(wěn)定、高選擇性檢測。
[Abstract]:Bipolar electrochemistry is a kind of electrochemical research method in which bipolarization electrode BPEE is used as working electrode. It includes two kinds of open bipolarization system and closed bipolarization system, which have redox or reduction reaction at the two ends of the electrode respectively. In this paper, we mainly study the closed bipolarization system. Based on the closed bipolarization principle, we are some neurotransmitters or active molecules in the brain, such as dopamine, dopamine. The electrochemical detection of ascorbic acid provides a new and effective method for detection of ascorbic acid. The specific work is summarized as follows: (1) We first studied the effects of electrode size and the concentration of matter at both ends of the electrode on the half-wave potential in a closed bipolar system. Then the cylindrical carbon fiber electrode filled with K_3Fe(CN)_6 solution was prepared by selecting the most suitable K_3Fe(CN)_6 concentration and electrode size for in situ detection of dopamine dadamine in the brain. The stability, sensitivity and half-wave potential of the electrode were compared with those of the conventional solution filled with KCl or Ru(NH3)6Cl3. Finally, it was proved that the prepared electrode had high stability for the detection of catecholamine in vivo. The advantages of high sensitivity and low damage to animals.) We have modified the closed bipolar electrode device in the traditional sense, that is, to remove the external applied voltage. The electrochemical materials which can be oxidized or reduced were added at the two ends of the electrode, and the potential difference between the two ends of the electrode was used to convert the system into a self-generated primary battery. The voltage and power are outputted by two ideal non-polarized electrodes (driving electrodes in closed bipolar systems). Under this design, we have studied the performance of HStut _ 2-O _ 2C _ S _ 4FeC _ S _ 4C _ S _ 4 C _ S _ 6O _ 2C _ T _ 6-O _ 2C _ T _ 3F _ C _ C _ C _ C _ C _ C _ C _ C _ C _ C _ T _ (6), and found that the output of power and voltage is better. 3) A closed double polarization system based on the modified principle of the primary cell. We have prepared a micro biosensor for the detection of ascorbic acid release in vivo. The carbon fiber electrode was filled with a certain concentration of K_3Fe(CN)_6 solution containing supporting electrolyte, and the multi-walled carbon nanotubes were modified on the electrode surface, which was used as the working electrode for the in-situ detection of ascorbic acid in vivo. We have studied the performance of the biosensor based on the principle of primary battery and found that this method can be used to detect ascorbic acid in brain with high stability and selectivity.
【學(xué)位授予單位】：湘潭大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：O657.1
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本文編號：1888693