本文選題：米諾地爾 + 毛細(xì)管區(qū)帶電泳　； 參考：《環(huán)境與健康雜志》2017年08期

【摘要】：目的建立化妝品中米諾地爾的毛細(xì)管區(qū)帶電泳(CZE)測(cè)定法。方法樣品經(jīng)提取液(甲醇∶2 mmol/L磷酸二氫鈉+3 mmol/L磷酸體積比為1∶9)提取后離心,過0.45μm膜后上機(jī)。采用未涂敷熔融石英毛細(xì)管[75μm×30.2 cm(有效長(zhǎng)度:20 cm)];分離緩沖液為20 mmol/L磷酸二氫鈉-30 mmol/L磷酸,pH 2.13;分離電壓為11 kV;檢測(cè)波長(zhǎng)為278 nm;進(jìn)樣壓力為0.5 psi;進(jìn)樣時(shí)間為3 s。結(jié)果在1~160 mg/L的線性范圍內(nèi),所得米諾地爾的回歸方程為y=151.12x+73.363,r=0.999 95,呈良好的線性關(guān)系。該方法的檢出限為0.3 mg/L,定量下限為1.0 mg/L,平均回收率在93.4%~95.9%之間,RSD在3.3%~3.7%之間。結(jié)論該方法簡(jiǎn)單、快速、準(zhǔn)確,可用于化妝品中米諾地爾含量的檢測(cè)。
[Abstract]:Objective to establish a capillary zone electrophoresis (CZE) method for the determination of minoxidil in cosmetics. Methods the sample was extracted from the extract solution (methanol: 2 mmol/L sodium dihydrogen phosphate 3 mmol/L phosphoric acid volume ratio 1: 9) and centrifuged. The uncoated fused quartz capillary [75 渭 m 脳 30.2 cm (effective length: 20 cm)] was used, the separation buffer was 20 mmol/L sodium dihydrogen phosphate -30 mmol/L phosphate pH 2.13, the separation voltage was 11 KV, the detection wavelength was 278nm, the injection pressure was 0.5 psii and the injection time was 3 s. Results within the linear range of 1: 160 mg/L, the regression equation of minodil was YJ 151.12x 73.363C 0.999 95, showing a good linear relationship. The detection limit of this method is 0.3 mg / L, the limit of quantification is 1.0 mg / L, and the average recovery is between 93.44% and 95.9% (RSD = 3.3%). Conclusion the method is simple, rapid and accurate, and can be used for the determination of minoxidil in cosmetics.
【作者單位】： 北京市疾病預(yù)防控制中心中心實(shí)驗(yàn)室食物中毒診斷溯源技術(shù)北京市重點(diǎn)實(shí)驗(yàn)室;北京市預(yù)防醫(yī)學(xué)研究中心;北京化工大學(xué)理學(xué)院;首都醫(yī)科大學(xué)公共衛(wèi)生學(xué)院;
【基金】：北京市衛(wèi)生系統(tǒng)高層次衛(wèi)生技術(shù)人才培養(yǎng)項(xiàng)目(2013-2-036)
【分類號(hào)】：O658.9;TQ658

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