本文選題：硒化銅 + 化學發(fā)光��； 參考：《西南大學》2017年碩士論文

【摘要】：近年來,由于具有優(yōu)越的催化活性、生物相容性、易自組裝等特質,金屬納米材料、非金屬納米材料、半導體納米材料均廣泛被引入發(fā)光體系中,將其成功用于生物分析中。這些研究在改進分析方法靈敏度、縮短分析時間等方面取得了重要進展。過去,化學發(fā)光的研究領域主要局限于分子、離子等體系。近年來,隨著納米科技的飛速發(fā)展,納米材料參與的液相化學發(fā)光反應受到廣泛關注。本論文介紹了常見的化學發(fā)光體系,并綜述了納米材料參與的化學發(fā)光體系及其在分析化學中的應用。實驗室發(fā)現(xiàn)半導體納米粒子硒化銅納米能夠作為催化劑增強液相魯米諾過氧化氫體系的化學發(fā)光信號,然而目前已經(jīng)報道的普遍應用的納米粒子主要集中在貴金屬納米粒子如納米金、銀等,關于硒化銅催化化學發(fā)光的研究報道極少。因此本論文將硒化銅納米粒子應用在液相化學發(fā)光體系中,研究了硒化銅納米粒子對魯米諾化學發(fā)光體系的催化作用,進一步討論了催化化學發(fā)光的機理并將其應用于生物分子的檢測中,結果表明分析的靈敏度得到進一步的提高,同時拓寬分析方法的線性范圍。具體的研究內容包括以下三個方面:(1)建立一種以硒化銅催化化學發(fā)光檢測膽固醇的新方法。膽固醇在膽固醇氧化酶的作用下生成過氧化氫,過氧化氫在硒化銅納米粒子存在的條件下和魯米諾發(fā)生化學發(fā)應,產生較強的化學發(fā)光信號,化學發(fā)光的信號強弱與膽固醇的濃度呈正相關的關系�；诖�,開發(fā)了一種借助于硒化銅催化效果的簡單快速檢測膽固醇的方法,并將該化學發(fā)光體系用于人體尿液中膽固醇含量的檢測。(2)建立一種通過調節(jié)硒化銅催化化學發(fā)光檢測溶菌酶的新方法。在合成硒化銅的基礎上,通過調節(jié)反應條件,探索了一種更快速的合成具有表面負電荷的硒化銅的方法。通過實驗發(fā)現(xiàn),該硒化銅納米粒子也具有優(yōu)良的催化化學發(fā)光的性質,在暗室中發(fā)現(xiàn)其能催化魯米諾-過氧化氫體系發(fā)出長時間的可視化化學發(fā)光。由于納米粒表面帶有負電荷,溶菌酶在其等電點以下帶正電荷,兩者通過靜電作用力相互作用,影響其聚集狀態(tài)及催化活性,不同濃度溶菌酶的加入和化學發(fā)光信號猝滅程度有正相關關系,從而建立了溶菌酶免標、靈敏、快速的檢測方法。該研究不僅建立了一種檢測溶菌酶的新方法,擴寬了金屬氧族化合物納米材料在分析檢測方面的應用。(3)合成了金包被硒化銅的納米復合物,具有化學發(fā)光活性。硒化銅不易修飾,本論文將金納米包被在硒化銅的表面,從而便于化學發(fā)光物質魯米諾通過Au-N鍵耦合在復合納米粒子表面。通過實驗證明了該復合納米粒子的成功合成,成功探索設計了一種簡單、快速的合成具有化學發(fā)光活性硒化銅-金-魯米諾花狀復合物的新方法,通過紫外、電子掃描顯微鏡等手段證明了該復合物的成功合成,該復合物具有良好的化學發(fā)光活性,有望將其應用于復雜生物體內活性氧的檢測。通過以上研究,本文建立了基于硒化銅作為催化劑的簡單、快速、靈敏的檢測新方法,探究具有高的化學發(fā)光效率的新體系,拓展了化學發(fā)光理論和應用研究范圍,為化學發(fā)光和納米材料的基礎研究以及化學發(fā)光在分析檢測應用方面提供一些新方法和新思路。
[Abstract]:In recent years, metal nanomaterials, non-metallic nanomaterials and semiconductor nanomaterials have been widely introduced into the luminescent system because of their superior catalytic activity, biocompatibility and easy self-assembly, and they have been successfully applied to biological analysis. These studies have been important in improving the sensitivity of analytical methods and reducing the time of analysis. In the past, the research field of chemiluminescence is mainly limited to molecules, ions and other systems. In recent years, with the rapid development of nanotechnology, the liquid phase chemiluminescence reaction of nanomaterials has attracted wide attention. This paper introduces the common chemiluminescence system, and summarizes the chemiluminescence system and its analysis in the analysis of nanomaterials. The laboratory found that the semiconductor nanoparticles can be used as the chemiluminescence signal of the catalyst enhanced liquid phase Lumino hydrogen peroxide system. However, the widely used nanoparticles are mainly concentrated in the noble metal nanoparticles, such as nano gold, silver, etc., on the catalytic chemiluminescence of copper selenide. The research reports are very few. Therefore, the copper selenide nanoparticles were used in the liquid chemiluminescence system, and the catalytic effect of copper selenide nanoparticles on the Lumino chemiluminescence system was studied. The mechanism of the catalytic chemiluminescence was further discussed and applied to the detection of biomolecules. The results showed that the sensitivity of the analysis was obtained. The following three aspects include: (1) a new method for the detection of cholesterol with copper selenide catalyzed chemiluminescence is established. The formation of hydrogen peroxide under the action of cholesterol oxidase in the presence of copper selenide, and the presence of copper selenide nanoparticles and Lu rice A chemical luminescence signal is produced and a strong chemiluminescence signal is produced. The signal intensity of chemiluminescence is positively related to the concentration of cholesterol. Based on this, a simple and rapid method for detecting cholesterol with the help of copper selenide catalysis is developed, and the chemiluminescent system is used for the detection of cholesterol content in human urine. (2) A new method for the detection of lysozyme by catalytic chemiluminescence of copper selenide was established. On the basis of the synthesis of copper selenide, a more rapid method for synthesis of copper selenide with negative surface charge was explored by adjusting the reaction conditions. It was found that the copper selenide nanoparticles also had excellent catalytic chemiluminescence properties. It is found in the dark room that it can catalyze the long time visual chemiluminescence of the luminol hydrogen peroxide system. Due to the negative charge of the nanoparticles, the lysozyme has positive charge below the isoelectric point. Both of them affect the aggregation and the catalytic activity through the electrostatic force, and the addition of different concentrations of lysozyme and chemical hair. There is a positive correlation between the degree of light signal quenching and the establishment of a rapid and sensitive method for detecting lysozyme. This study not only establishes a new method for detecting lysozyme, but also broadens the application of nanomaterials in the analysis and detection of metal oxide compounds. (3) the synthesis of gold coated copper selenide nanocomposites with Chemiluminescence Copper selenide was not easy to be modified. In this paper, gold nanoparticles were coated on the surface of copper selenide, so that the chemiluminescent material, Lumino, was coupled to the surface of the composite nanoparticles through the Au-N bond. The successful synthesis of the composite nanoparticles was proved by experiments. A simple and rapid synthesis of chemiluminescent active selenium was designed and designed. A new method of copper gold luminol flower like complex has been proved by UV and electron scanning microscopy. The composite has good chemiluminescence activity and is expected to be applied to the detection of reactive oxygen in complex organisms. Through the above study, copper selenide is established as a catalyst. A new rapid and sensitive detection method is used to explore new systems with high chemiluminescence efficiency, which extends the scope of chemiluminescence theory and application, and provides some new methods and new ideas for the basic research of chemiluminescence and nanomaterials and the application of chemiluminescence in analysis and detection.

【學位授予單位】：西南大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.3;O643.3

【參考文獻】

相關期刊論文 前8條

1 李向堂;張禮春;趙書林;;基于微流控芯片電泳化學發(fā)光檢測人單個紅細胞中血紅蛋白的含量[J];分析測試學報;2015年03期

2 牛衛(wèi)芬;呂九如;;高錳酸鉀-魯米諾化學發(fā)光體系分子印跡-后化學發(fā)光法測定奮乃靜[J];分析化學;2007年02期

3 劉清慧;呂九如;馮娜;;鐵氰化鉀-魯米諾體系后化學發(fā)光反應及其分析應用研究——分子印跡-后化學發(fā)光法測定雙嘧達莫[J];高等學校化學學報;2006年06期

4 范順利;張立科;魏彥林;林金明;;高效液相色譜-高錳酸鉀氧化化學發(fā)光法測定水中的痕量苯二酚[J];色譜;2006年02期

5 何云華,呂九如,張紅鴿,杜建修;分子印跡-化學發(fā)光分析法測定安乃近[J];高等學�；瘜W學報;2005年04期

6 林金明;化學發(fā)光色譜柱后檢測技術及其應用[J];色譜;2003年04期

7 林金明;活性氧的化學發(fā)光測定法[J];環(huán)境科學學報;2003年02期

8 林金明,屈鋒,單孝全;活性氧測定的基本原理與方法[J];分析化學;2002年12期

相關博士學位論文 前2條

1 段春鳳;金屬納米誘導的化學發(fā)光體系的設計及在免疫分析中的應用[D];中國科學技術大學;2009年

2 郭吉兆;貴金屬納米粒子參與的化學發(fā)光[D];中國科學技術大學;2007年

相關碩士學位論文 前2條

1 劉博;金屬鉑納米催化的光澤精化學發(fā)光新體系及其應用[D];中國科學技術大學;2010年

2 白文玲;毛細管電泳化學發(fā)光聯(lián)用技術在藥物分析中的應用[D];廣西師范大學;2006年

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