本文選題：NADH + 3-氨基苯硼��； 參考：《西北大學(xué)》2017年碩士論文

【摘要】：煙酰胺腺嘌呤二核苷酸(NADH)是人體中最重要輔酶之一,在人體的許多氧化還原反應(yīng)中起著傳遞電子和質(zhì)子的作用,研究NADH的電化學(xué)氧化對了解生命體系的電荷轉(zhuǎn)移有著非常重要的意義。但由于NADH在裸電極表面的過電位較高,因此,在分析實際樣品時會受到許多電活性物質(zhì)的干擾,從而導(dǎo)致電極表面鈍化、靈敏度降低以及重現(xiàn)性變差等問題。通過對電極表面進(jìn)行修飾,不僅可以降低NADH的過電位,同時也可以避免干擾物質(zhì)的影響。本論文基于硼親作用實現(xiàn)NADH在電極表面的富集,結(jié)合吩嗪硫酸甲酯(PMS)對NADH的催化,實現(xiàn)對了在低電位下NADH的高靈敏檢測;研究了氧化還原介體與二價陽離子對NADH的協(xié)同電催化作用;共分為三章:1.第一章主要對NADH及其在人體的作用以及NADH電化學(xué)傳感器的研究進(jìn)展進(jìn)行了簡單的介紹。2.第二章采用硼酸功能化多壁碳納米管(MWCNTs)吸附吩嗪硫酸甲酯(PMS)作為電極修飾材料,構(gòu)建了低電位高靈敏的NADH電化學(xué)傳感器。硼酸作為識別單元可有效實現(xiàn)NADH在電極表面的富集;同時,電極表面吸附的PMS對富集的NADH產(chǎn)生電催化作用。該傳感器不僅具有較高的靈敏度,而且可在較低電位下實現(xiàn)檢測,有效地避免了共存物質(zhì)的干擾。3.第三章研究了氧化還原介體與二價陽離子對NADH的協(xié)同電催化作用。將PMS固定在MWCNTs和離子液體(IL)復(fù)合電極表面,MWCNTs大的比表面積增加了PMS在電極表面的負(fù)載量,IL較高的導(dǎo)電性加速了 NADH與電極之間的電子轉(zhuǎn)移。在以上修飾電極上研究了七種二價陽離子對NADH的電催化作用,研究表明,Ca~(2+) 的催化效果最好,催化電流增大了 2.3倍,從而實現(xiàn)了對NADH高靈敏的電化學(xué)檢測。
[Abstract]:Nicotinamide adenine dinucleotide (NADH) is one of the most important coenzymes in human body and plays an important role in the transport of electrons and protons in many redox reactions. It is very important to study the electrochemical oxidation of NADH for understanding the charge transfer of the living system. However, due to the high overpotential of NADH on the bare electrode surface, many electroactive substances interfere with the analysis of the actual sample, which leads to the passivation of the electrode surface, the decrease of the sensitivity and the poor reproducibility of the electrode surface. By modifying the electrode surface, not only the overpotential of NADH can be reduced, but also the influence of interfering substances can be avoided. In this paper, the enrichment of NADH on the electrode surface based on boron lipophilicity and the catalytic activity of phenazine methyl ester sulfate (PMSs) on NADH were carried out to achieve the high sensitivity detection of NADH at low potential. The synergistic electrocatalytic effect of redox and divalent cations on NADH was studied, which was divided into three chapters: 1: 1. In the first chapter, the research progress of NADH and its role in human body and the electrochemical sensor of NADH are briefly introduced. In chapter 2, a low potential and high sensitivity NADH electrochemical sensor was constructed by using boric acid functionalized multiwalled carbon nanotubes (MWCNTs) to adsorb Phenazine Methyl Sulfate (Phenazine Sulfate) as electrode modified materials. Boric acid can effectively realize the enrichment of NADH on the electrode surface and the PMS adsorbed on the electrode surface can produce electrocatalytic effect on the enriched NADH. The sensor not only has high sensitivity, but also can be detected at lower potential, effectively avoiding the interference of coexisting substances. 3. In chapter 3, the synergistic electrocatalysis of NADH by redox and divalent cations was studied. The large specific surface area of PMS on the surface of MWCNTs and ionic liquid PMS composite electrode increases the loading amount of PMS on the electrode surface and the high conductivity of IL accelerates the electron transfer between NADH and the electrode. The electrocatalytic effect of seven kinds of divalent cations on NADH was studied at the above modified electrode. The results showed that the catalytic effect was the best, and the catalytic current increased 2.3 times, thus the highly sensitive electrochemical detection of NADH was realized.
【學(xué)位授予單位】：西北大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：Q75;O657.1

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