發(fā)布時(shí)間：2018-05-03 15:08

  本文選題：碳點(diǎn) + 熒光��； 參考：《華東師范大學(xué)》2017年博士論文

【摘要】：熒光碳點(diǎn)由于其良好的生物兼容性和制備方法的簡(jiǎn)便性受到了廣泛的關(guān)注。本論文主要采用電化學(xué)法和水熱法制備了一系列熒光碳點(diǎn)。研究了碳點(diǎn)作為能量給體用于DNA雜化的檢測(cè)、反應(yīng)物對(duì)碳點(diǎn)性能的影響、聚集效應(yīng)對(duì)碳點(diǎn)熒光性質(zhì)的影響。具體如下:1.采用碳棒作為工作電極和對(duì)電極,銀/氯化銀作為參比電極,通過(guò)電化學(xué)氧化的方法,制備了綠色熒光的碳點(diǎn),并對(duì)其形貌和熒光性能進(jìn)行了表征。將所制備的熒光碳點(diǎn)作為能量給體,能過(guò)帶正電的聚合物聚二甲基二烯丙基氯化銨(PDADMAC)作為電荷橋梁,與Cy3熒光染料標(biāo)記的單鏈DNA通過(guò)電荷間的作用形成"三明治"結(jié)構(gòu),構(gòu)建了基于熒光共振能量轉(zhuǎn)移(FRET)的DNA雜化檢測(cè)平臺(tái),實(shí)現(xiàn)了對(duì)目標(biāo)DNA的定量檢測(cè)。2.以磷�；宜崛阴樵�,在磷酸的存在下通過(guò)水熱法,制備了磷摻雜的、水溶性的熒光碳點(diǎn)。研究了磷摻雜碳點(diǎn)的熒光性質(zhì),發(fā)現(xiàn)了隨著熒光碳點(diǎn)的濃度增大,碳點(diǎn)的熒光發(fā)射峰紅移;降低濃度導(dǎo)致熒光發(fā)射峰藍(lán)移。通過(guò)實(shí)現(xiàn)證實(shí)了所發(fā)現(xiàn)的熒光紅移現(xiàn)象是由于碳點(diǎn)的聚集所導(dǎo)致。3.研究了反應(yīng)物對(duì)所得到的氮摻雜熒光碳點(diǎn)性能的影響。采用檸檬酸或者葡萄糖作為碳源,4,7,10-三氧-1,13-十三烷二胺或者多巴胺作為氮源,兩兩組合,通過(guò)水熱法制備了四種碳點(diǎn)。通過(guò)透射電子顯微鏡(TEM)和原子力顯微鏡(AFM)對(duì)所制備的碳納米點(diǎn)進(jìn)行形貌表征之后,通過(guò)對(duì)它們光學(xué)性質(zhì),電化學(xué)性質(zhì)的研究,發(fā)現(xiàn)反應(yīng)原料不僅僅影響其熒光性能,也影響了其它的理化性能,如pH敏感性、光伏性能。其中用4,7,10-三氧-1,13-十三烷二胺作為氮源制備的碳點(diǎn),比多巴胺作為氮源所制備的碳點(diǎn)具有更高的熒光量子產(chǎn)率;而用多巴胺作為氮源制備得到的碳點(diǎn),因?yàn)槠浔砻婧卸喟桶坊蛘叨喟桶奉愃莆锏慕Y(jié)構(gòu),具有更高的pH敏感性。此外,用多巴胺作為氮源的所制備的兩種碳點(diǎn)不僅具有熒光特性,同時(shí)還表現(xiàn)出電化學(xué)活性,為通過(guò)原料的選擇制備多功能熒光碳點(diǎn)提供了一個(gè)新的思路。4.采用具有電化學(xué)活性的溴化乙錠(EB)作為反應(yīng)物,通過(guò)水熱法制備了熒光碳點(diǎn),其熒光峰位于510 nm,并且不隨激發(fā)波長(zhǎng)的變化而變化。循環(huán)伏安法測(cè)試結(jié)果表明,在pH為0.54時(shí),所得到的碳點(diǎn)于-0.63出現(xiàn)了一還原峰,并且隨著pH的增大,還原峰正移。該特性可用于pH變化檢測(cè)。
[Abstract]:Fluorescent carbon points have been widely concerned because of their good biocompatibility and the simplicity of preparation methods. In this paper, a series of fluorescent carbon dots were prepared by electrochemical method and hydrothermal method. The effects of carbon dots as energy donors on the detection of DNA hybrid, the effect of the reactants on the carbon point energy and the carbon point fluorescence properties of the carbon dots were studied. The effects are as follows: 1. using carbon rods as working electrodes and opposite electrodes, silver / silver chloride as a reference electrode, the carbon points of green fluorescence are prepared by electrochemical oxidation, and their morphology and fluorescence properties are characterized. The prepared fluorescent carbon points can be used as an energy donor and can lead to a positive polymer of poly (two methyl diene). Propyl ammonium chloride (PDADMAC) is used as a charge bridge and the single strand DNA marked by Cy3 fluorescent dye forms a "sandwich" structure. A DNA hybrid detection platform based on fluorescence resonance energy transfer (FRET) is constructed. The quantitative detection of the target DNA is achieved by using three ethyl phosphoacetoacetate as the raw material and the presence of phosphoric acid in the presence of phosphoric acid. The fluorescence properties of phosphorus doped carbon dots were studied by over hydrothermal method. The fluorescence properties of phosphorus doped carbon point were studied. It was found that the fluorescence emission peak of the carbon point was red shift with the increase of the concentration of the fluorescent carbon point, and the decrease of the concentration resulted in the blue shift of the fluorescence emission peak. The results showed that the fluorescence redshift found by the fluorescence was caused by the aggregation of carbon points. 3. the effects of the reactant on the properties of nitrogen doped fluorescent carbon were investigated. Four kinds of carbon points were prepared by using citric acid or glucose as carbon source, 4,7,10- three oxygen -1,13- thirteen alkane two amine or dopamine as nitrogen source, and 22 combinations by hydrothermal method. The preparation was prepared by transmission electron microscopy (TEM) and atomic force microscopy (AFM). After characterization of the carbon nanodots, through the study of their optical properties and electrochemical properties, it is found that the reaction material not only affects its fluorescence properties, but also affects other physical and chemical properties, such as pH sensitivity and photovoltaic performance. The carbon points prepared by 4,7,10- three oxygen -1,13- thirteen alkanes as nitrogen source are compared with dopamine as nitrogen source. The carbon dots prepared have higher fluorescence quantum yields, and the carbon points produced by dopamine as a nitrogen source, because the surface contains dopamine or dopamine analogues, with higher pH sensitivity. In addition, the two kinds of carbon dots prepared with dopamine as nitrogen sources have not only fluorescence characteristics but also electrochemistry. The study activity provides a new idea for the preparation of multi-functional fluorescent carbon points through the selection of raw materials..4. uses electrochemically active ethidium bromide (EB) as a reactant, and the fluorescent carbon point is prepared by hydrothermal method. Its fluorescence peak is located at 510 nm and does not change with the change of the excitation wavelength. Cyclic voltammetry test results show that in pH At 0.54, a reduction peak appeared in -0.63, and the reduction peak shifted positively with the increase of pH. This characteristic can be used for pH change detection.

【學(xué)位授予單位】：華東師范大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.3

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本文編號(hào)：1838987