本文選題：精胺 + 亞精胺�。� 參考：《光譜學(xué)與光譜分析》2017年06期

【摘要】：熒光單分子實驗結(jié)果表明溫度能夠促進(jìn)DNA凝聚,隨著溫度升高,精胺(亞精胺)-DNA凝聚體系中DNA凝聚構(gòu)象趨于更加緊致、有序。為探究溫度對不同高價離子-DNA凝聚體系的影響,利用自行搭建的變溫紫外分光光度計,通過系統(tǒng)研究熒光單分子實驗中采用的精胺(亞精胺)-DNA凝聚體系及去離子水中DNA在260nm處特征吸收值隨溫度變化情況,確定了DNA凝聚構(gòu)象與其260nm處特征吸收值之間的對應(yīng)關(guān)系為:DNA凝聚構(gòu)象越緊致、有序,其對應(yīng)的260nm處紫外吸收值越低。在此基礎(chǔ)上,系統(tǒng)地研究了DNA凝聚實驗中常用四種鈷胺化合物-DNA凝聚體系隨溫度變化情況,結(jié)果表明三氯六胺合鈷與精胺(亞精胺)類似,其對應(yīng)的DNA凝聚體系在260nm特征吸收值,隨著溫度升高而逐漸降低,即DNA凝聚構(gòu)象趨于更加緊致、有序。而三(乙二胺)氯化鈷、反式雙(乙二胺)氯化鈷、五胺氯化鈷的情況卻不同,與其對應(yīng)的DNA凝聚體系在260nm特征吸收值,隨著溫度升高,呈現(xiàn)先增加,再降低,然后再增加的規(guī)律。
[Abstract]:The results of fluorescence monolayer experiments show that temperature can promote DNA condensation, and with the increase of temperature, the conformation of DNA condensation in spermidine (spermidine) -DNA condensation system tends to be more compact and ordered. In order to investigate the effect of temperature on different high valence ions-DNA condensate system, a self-built variable temperature ultraviolet spectrophotometer was used. The characteristic absorption values of DNA at 260nm in deionized water and spermine (spermidine) condensate system used in fluorescence monolayer experiments were systematically studied. The relation between the conformation of DNA condensation and the characteristic absorption value of 260nm is determined as follows: the tighter the conformation of DNA condensation is, the more ordered the conformation is, the lower the UV absorption value of the corresponding 260nm is. On the basis of this, four kinds of cobalt amine compounds in common use in DNA condensation experiment were studied systematically. The results showed that cobalt trichlorohexamethylamine was similar to spermidine (spermidine), and the results showed that the cobalt trichlorohexylamine was similar to the spermidine (spermidine). The characteristic absorption of the corresponding DNA condensate system decreases with the increase of temperature, that is, the conformation of DNA condensation tends to be more compact and orderly. However, the case of tris (ethylenediamine) cobalt chloride, trans bis (ethylenediamine) cobalt chloride and pentylamine cobalt chloride is different. With the increase of temperature, the characteristic absorption value of the corresponding DNA condensate system in 260nm increases first and then decreases. Then increase the law.
【作者單位】： 貴州大學(xué)生命科學(xué)學(xué)院;貴州大學(xué)物理學(xué)院軟凝聚態(tài)物理實驗室;
【基金】：國家自然科學(xué)基金項目(11047022,11204045,11464004) 貴州省社會發(fā)展攻關(guān)項目(SY20123089,SZ20113069) 中國博士后面上項目(2014M562341) 教育部重點科技項目(2012152) 貴州省教育廳創(chuàng)新群體項目((2014)32) 西部之光項目(2015) 貴州大學(xué)實驗室開放課題(2016)資助
【分類號】：O629.74;O657.32

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