本文選題：傅里葉變換紅外光譜 + 三七�。� 參考：《光譜學(xué)與光譜分析》2016年08期

【摘要】：野生近緣種在豐富種質(zhì)資源以及改善栽培物種品質(zhì)產(chǎn)量方面具有十分重要的意義和作用。采用傅里葉變換紅外光譜對(duì)三七及其野生近緣種親緣關(guān)系進(jìn)行探討,以期為三七的品種改良及種質(zhì)資源開(kāi)發(fā)利用提供理論基礎(chǔ)。采集三七及其4個(gè)野生近緣種(珠子參、屏邊三七、越南人參、羽葉三七)、三七總皂苷標(biāo)準(zhǔn)品紅外光譜,將三七及其野生近緣種紅外光譜進(jìn)行自動(dòng)基線校正、平滑、縱坐標(biāo)歸一化、二階導(dǎo)數(shù)預(yù)處理,結(jié)合主成分分析、偏最小二乘判別分析及系統(tǒng)聚類分析進(jìn)行親緣關(guān)系研究。結(jié)果顯示,三七與三七總皂苷標(biāo)準(zhǔn)品紅外光譜在3 400,2 930,1 635,1 385,1 075,927 cm~(-1)等附近存在多個(gè)共有峰,推測(cè)這些共有峰強(qiáng)度與皂苷含量之間有一定關(guān)系;三七及其野生近緣種原始光譜較為相似,存在C-H,C=O,O—H,C—N,C—O等官能團(tuán)吸收峰,不同物種的二階導(dǎo)數(shù)紅外光譜指紋區(qū)(1 800~500 cm~(-1))具有較大差異,如1 385和784 cm~(-1)等特征吸收峰差異明顯;選取指紋區(qū)光譜數(shù)據(jù)進(jìn)行主成分分析、偏最小二乘判別分析,通過(guò)比對(duì)兩種方法的3D得分圖,偏最小二乘判別分析對(duì)物種的區(qū)分效果優(yōu)于主成分分析;前6個(gè)主成分?jǐn)?shù)據(jù)的系統(tǒng)聚類分析表明,三七與越南人參、珠子參親緣關(guān)系較近,與屏邊三七、羽葉三七親緣關(guān)系較遠(yuǎn)。利用傅里葉變換紅外光譜結(jié)合化學(xué)計(jì)量學(xué)的方法能有效對(duì)三七及其野生近緣種進(jìn)行親緣關(guān)系分析,同時(shí)為藥用植物種質(zhì)資源相關(guān)研究提供借鑒與參考。
[Abstract]:Wild relative species play an important role in enriching germplasm resources and improving the quality and yield of cultivated species. The relationship between Panax notoginseng and its wild relatives was studied by Fourier transform infrared spectroscopy (FTIR) in order to provide theoretical basis for variety improvement and germplasm resources development and utilization of Panax notoginseng. The infrared spectra of Panax notoginseng and its four wild relative species (Panax japonicus, Panax notoginseng, Panax ginseng, Panax notoginseng, Panax notoginseng) were collected, and the infrared spectra of Panax notoginseng and its wild relatives were calibrated automatically and smoothly. The genetic relationship is studied by means of ordinate normalization, second derivative preprocessing, principal component analysis, partial least squares discriminant analysis and systematic cluster analysis. The results showed that there were several common peaks in the vicinity of 3400 ~ (2 930) C ~ (-1) C ~ (-1) and total saponins of Panax notoginseng and Panax notoginseng. It was inferred that there was a certain relationship between the intensity of these common peaks and the content of saponins. The original spectra of Panax notoginseng and its wild relative species were similar, and there were absorption peaks of functional groups, such as C-Haphnianthus OOOOHZOHZON C-O, and the second derivative infrared fingerprint region of different species was 1 800 ~ 500 cm ~ (-1) ~ 1 ~ (-1), and there was a great difference in the original spectra of Panax notoginseng and its wild relative species. For example, there are obvious differences in absorption peaks between 1 385 and 784 cm ~ (-1), the spectral data of fingerprint region are selected for principal component analysis, partial least squares discriminant analysis, and 3D score map of the two methods are compared. Partial least squares discriminant analysis was superior to principal component analysis in distinguishing species, and the systematic cluster analysis of the first six principal components data showed that Panax notoginseng was closely related to Panax ginseng and Radix Ginseng, and Panax notoginseng was closely related to Panax notoginseng. The phylogenetic relationship of Panax notoginseng was far away. Fourier transform infrared spectroscopy (FTIR) combined with chemometrics can effectively analyze the genetic relationship of Panax notoginseng and its wild relative species and provide reference for the study of medicinal plant germplasm resources.
【作者單位】： 云南中醫(yī)學(xué)院中藥學(xué)院;云南省農(nóng)業(yè)科學(xué)院藥用植物研究所;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81260610,81460581)資助
【分類號(hào)】：S567.236;O657.33
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本文編號(hào)：1831291