發(fā)布時間：2018-04-27 03:24

  本文選題：雜色曲霉毒素 + 離子液體　； 參考：《天津科技大學》2017年碩士論文

【摘要】：雜色曲霉毒素(ST)是一種霉菌毒素,主要是由雜色曲霉、構巢曲霉和離孺孢霉等真菌產(chǎn)生的有毒次級代謝產(chǎn)物,廣泛存在于自然界中,主要對小麥、玉米、花生、大豆等糧食作物及動物飼料造成污染,是最常見的霉菌毒素污染物之一,人和畜禽攝入含有雜色曲霉毒素的食物會引起廣泛的毒性效應。所以建立雜色曲霉毒素的檢測方法具有重要意義。本論文合成了離子液體1-乙烯基-3-丙胺咪唑氫溴酸鹽和1-乙烯基-3-丁烯咪唑溴鹽,分別作為印跡過程中的功能單體和交聯(lián)劑,制備了兩種對ST具有選擇性的分子印跡聚合物。實驗以目標物結構類似物1,8-二羥基蒽醌作為ST替代模板,1-乙烯基-3-丙胺咪唑氫溴酸鹽作為功能單體,1-乙烯基-3-丁烯咪唑溴鹽作為交聯(lián)劑,偶氮二異丁腈為引發(fā)劑,采用原位聚合法成功制備了對ST具有特異性吸附的印跡聚離子液體毛細管整體柱。以1,8-二羥基蒽醌作為ST的替代模板,1-乙烯基-3-丙胺咪唑氫溴酸鹽作為功能單體,1-乙烯基-3-丁烯咪唑溴鹽作為交聯(lián)劑,偶氮二異丁腈為引發(fā)劑,采用本體聚合法制備了對ST具有特異性吸附性能的印跡聚離子液體聚合物,并將該分子印跡聚合物作為固相萃取的填充材料。采用原位聚合法直接在毛細管柱中合成出具有空穴結構的毛細管分子印跡整體柱。通過掃描電鏡表征顯示,所制備的毛細管整體柱內部結構均勻,孔隙度適中。采用電色譜模式對雜色曲霉毒素和其結構類似物黃曲霉毒素B1進行了分離。通過對分離條件的優(yōu)化實現(xiàn)了雜色曲霉毒素和黃曲霉毒素B1的快速分離。通過本體聚合法制備的印跡聚離子液體聚合物,利用掃描電鏡對制備的印跡聚離子液體聚合物進行了結構形態(tài)表征,通過吸附實驗進行了吸附性能表征。實驗結果表明,制備的聚離子液體印跡聚合物對雜色曲霉毒素具有較好的吸附效果。將聚離子液體印跡聚合物作為固相萃取材料,建立了雜色曲霉毒素分子印跡固相萃取與高效液相色譜聯(lián)用的方法,并應用于對實際樣品的檢測。
[Abstract]:Aspergillus chromotoxin (STT) is a mycotoxin, mainly a toxic secondary metabolite produced by Aspergillus heterochromoides, Aspergillus monocystis and Aspergillus sp., and other fungi. It is widely found in nature, mainly in wheat, corn, peanut, etc. Soybean and other food crops and animal feed pollution is one of the most common mycotoxin pollutants human and poultry intake of food containing aspergillus mutans can cause a wide range of toxic effects. Therefore, it is of great significance to establish a method for the detection of aspergillus mutans. In this paper, ionic liquids 1-vinyl-3-propylaminoimidazole hydrobromide and 1-vinyl-3-butene imidazolium bromide were synthesized. As functional monomers and crosslinkers in the imprinting process, two kinds of molecularly imprinted polymers with selectivity to St were prepared. In this experiment, the target structure analogue, 1o 8-dihydroxyanthraquinone, was used as a substitute for St template, 1-vinyl-3-propylaminoimidazole hydrobromate as the functional monomer 1-vinyl-3-butylene imidazolium bromide as crosslinking agent, and azodiisobutyronitrile as initiator, and azodiisobutyronitrile as initiator. Imprinted polyionic liquid capillary monolithic column with specific adsorption of St was successfully prepared by in situ polymerization. 1-vinyl-3-propylaminoimidazolium hydrobromide was used as a functional monomer, 1-vinyl-3-butenyl imidazolium bromide as crosslinking agent and azodiisobutyronitrile as initiator. The imprinted polyionic liquid polymer with specific adsorption property for St was prepared by bulk polymerization, and the molecularly imprinted polymer was used as the filling material for solid phase extraction (SPE). A capillary molecularly imprinted monolithic column with hole structure was synthesized by in-situ polymerization. Scanning electron microscopy (SEM) showed that the capillary column had a uniform internal structure and a moderate porosity. The hybrid aspergillus toxin and its structural analogue aflatoxin B1 were separated by electrochromatography. The rapid separation of aflatoxin and aflatoxin B1 was achieved by optimizing the separation conditions. The imprinted polyionic liquid polymer was prepared by bulk polymerization. The structure and morphology of the imprinted polyionic liquid polymer were characterized by scanning electron microscope. The adsorption properties of the imprinted polyionic liquid polymer were characterized by adsorption experiments. The experimental results show that the polyionic liquid imprinted polymer has a good adsorption effect on the heterochromatic aspergillus toxin. The polyionic liquid imprinted polymer was used as solid phase extraction material to establish the method of molecular imprinted solid phase extraction of aspergillus chromotoxin and high performance liquid chromatography (HPLC), and the method was applied to the detection of real samples.
【學位授予單位】：天津科技大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TS207.5;O658.2

【參考文獻】

相關期刊論文 前4條

1 江飛劍;王海彬;楊承剛;韋國棟;柯世省;李娜;章根寶;張平;;雜色曲霉素雙抗夾心ELISA檢測方法的建立[J];吉林農業(yè);2015年07期

2 徐存英;華一新;段云彪;張鵬翔;;離子液體存在下銀的電沉積及其表面增強拉曼散射活性研究[J];光譜學與光譜分析;2009年11期

3 蔡琪,鮮躍仲,李輝,張仰明,湯杰,金利通;采用離子液體的二氧化硫電化學傳感器的研究[J];華東師范大學學報(自然科學版);2001年03期

4 趙寶玉,曹光榮;雜色曲霉毒素研究進展[J];動物醫(yī)學進展;1997年02期

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本文編號：1808968