本文選題：反St(o|)kes發(fā)光 + 上轉(zhuǎn)換納米粒子�。� 參考：《湖南大學(xué)》2016年碩士論文

【摘要】：自二十世紀(jì)90年代末開始,上轉(zhuǎn)換納米材料的領(lǐng)域不斷發(fā)展,并逐步變成納米科學(xué)最活躍的研究領(lǐng)域之一。在過(guò)去20年里發(fā)表的大量上轉(zhuǎn)換納米材料的論文證明,很多研究團(tuán)隊(duì)在這個(gè)領(lǐng)域做出了大量努力。上轉(zhuǎn)換納米材料在近紅外光激發(fā)下,可以發(fā)射出紫外、可見或近紅外光,即反Stokes發(fā)光。這種獨(dú)特的發(fā)光性質(zhì),排除了生物樣品的背景熒光和來(lái)自生物系統(tǒng)的光散射,因此信噪比高。目前上轉(zhuǎn)換納米粒子可用于生物標(biāo)記、光動(dòng)力學(xué)治療和生物成像等領(lǐng)域。本論文把上轉(zhuǎn)換納米粒子應(yīng)用于免疫層析技術(shù),實(shí)驗(yàn)結(jié)果表明,這在一定程度上提高了免疫層析方法的靈敏性和檢測(cè)準(zhǔn)確性。在論文里,我們?cè)敿?xì)描述了上轉(zhuǎn)換納米粒子的合成過(guò)程和修飾方法,并把它與免疫層析技術(shù)相結(jié)合去檢測(cè)C-反應(yīng)蛋白。(1)本課題組對(duì)上轉(zhuǎn)換納米材料的合成原本已有較豐富的實(shí)踐經(jīng)驗(yàn),在此基礎(chǔ)上,我們合成出兩種疏水的上轉(zhuǎn)換納米粒子,即藍(lán)光NaYF_4:Yb,Tm/NaYF_4和綠光NaYF_4:Yb,Er/NaYF_4。為方便進(jìn)一步與生物分子偶聯(lián),我們把油溶性的兩種納米粒子通過(guò)表面配體吸附法,即納米晶體表面疏水基團(tuán)和高分子疏水碳鏈之間的疏水相互作用,使納米晶體吸附上一種兩親性嵌段高分子。高分子親水的一端裸露在外面,親水端豐富的羧基增加了納米粒子的水溶性,也方便進(jìn)一步與生物分子偶聯(lián)。通過(guò)透射電鏡可以發(fā)現(xiàn),納米粒子幾乎全部包覆上一層約2 nm厚的高分子膜。通過(guò)實(shí)驗(yàn)驗(yàn)證,證明這種表面修飾法耗時(shí)少,全程約30 min,操作簡(jiǎn)單,且修飾好的納米粒子在水溶液里不易團(tuán)聚,就目前來(lái)說(shuō),是效率較高且較容易的表面修飾方法。(2)免疫層析技術(shù),是基于抗原抗體的特異性識(shí)別,通過(guò)毛細(xì)管虹吸作用,使液體樣品在試紙條上橫向流動(dòng),液體樣品里的目標(biāo)物被分析膜上捕獲蛋白捕獲,最后通過(guò)檢測(cè)免疫試紙條的檢測(cè)線的熒光強(qiáng)弱來(lái)判斷目標(biāo)物濃度的方法。我們把上轉(zhuǎn)換納米粒子作為熒光探針應(yīng)用于免疫層析技術(shù),再檢測(cè)人體內(nèi)C-反應(yīng)蛋白。結(jié)果表明,該方法不僅保留了免疫層析試紙條操作簡(jiǎn)單,經(jīng)濟(jì)實(shí)惠的優(yōu)點(diǎn),而且應(yīng)用上轉(zhuǎn)換納米粒子為熒光探針,提高了C-反應(yīng)蛋白的檢測(cè)靈敏度和準(zhǔn)確性。并且,它可以被用于更多種類的抗原樣品的半定量和定量檢測(cè)中,具有較好的市場(chǎng)應(yīng)用前景。
[Abstract]:Since the late 1990s, the field of up-conversion nanomaterials has been developing and gradually becoming one of the most active research fields in nanoscience. Papers published over the past 20 years on up-conversion nanomaterials have demonstrated that many research teams have made a lot of effort in this area. The up-conversion nanocrystalline materials can emit ultraviolet, visible or near-infrared light, that is, anti- luminescence, under the excitation of near-infrared light. This unique luminescent property excludes the background fluorescence of biological samples and light scattering from biological systems, so the signal-to-noise ratio (SNR) is high. Up-conversion nanoparticles can be used in biomarkers, photodynamic therapy and biometric imaging. In this paper, the up-conversion nanoparticles were used in immunochromatography. The experimental results show that the sensitivity and accuracy of immunochromatography are improved to some extent. In this paper, we describe the synthesis process and modification methods of up-conversion nanoparticles in detail. And combine it with immunochromatography to detect C- reactive protein. (1) our team has had rich practical experience in the synthesis of up-conversion nanomaterials. On this basis, we have synthesized two kinds of hydrophobic up-conversion nanoparticles. That is, the blue light NaYF4: YbBU Tm / NaYFANG4 and the green NaYFs 4: Yb/ / NaYF4. In order to facilitate further coupling with biomolecules, two oil-soluble nanoparticles were adsorbed by surface ligands, that is, the hydrophobic interaction between the hydrophobic groups on the surface of nanocrystals and the hydrophobic carbon chains of polymers. An amphiphilic block polymer is adsorbed on nanocrystalline crystals. The hydrophilic end of the polymer is exposed to the outside, and the rich carboxyl groups at the hydrophilic end increase the water solubility of the nanoparticles and facilitate further coupling with biomolecules. It was found by TEM that almost all of the nanoparticles were coated with a polymer film about 2 nm thick. The experimental results show that the surface modification method takes less time, takes about 30 mins, is simple to operate, and the modified nanoparticles are not easy to reunite in aqueous solution. It is an efficient and easy surface modification method. It is based on the specific recognition of antigens and antibodies, and makes liquid samples flow transversely on the test paper by siphon action of capillary tube. The target in the liquid sample is captured by the captured protein on the analytical membrane. Finally, the method of judging the concentration of the target material by detecting the fluorescence intensity of the detection line of the immune test strip is presented. We applied up-conversion nanoparticles as fluorescent probes to immunochromatography to detect C-reactive proteins in human body. The results show that this method not only preserves the advantages of simple operation and economical operation of immunochromatographic strip, but also improves the sensitivity and accuracy of C-reactive protein detection by using up-conversion nanoparticles as fluorescent probes. Moreover, it can be used in the semi-quantitative and quantitative detection of more kinds of antigens, and has a good market prospect.
【學(xué)位授予單位】：湖南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：O658.1;TB383.1
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本文編號(hào)：1778588