本文選題：三唑類殺菌劑 + 金屬配合物�。� 參考：《西北大學(xué)》2017年碩士論文

【摘要】：三唑類殺菌劑由于高效、廣譜、低毒的優(yōu)良特性,使其在農(nóng)業(yè)殺菌方面具有突出優(yōu)勢,但是廣泛的使用使菌種已經(jīng)對(duì)其產(chǎn)生了抗藥性,因此,尋找高效的、高安全性的、環(huán)境友好的新型真菌類殺菌劑勢在必行。近年來,一些研究者發(fā)現(xiàn)農(nóng)藥殺菌劑和過渡金屬通過配位反應(yīng)形成金屬配合物,可以提高殺菌活性同時(shí)降低毒性,減小對(duì)環(huán)境的污染�；谏鲜鰞�(yōu)點(diǎn),本文以烯效唑和多效唑?yàn)榕潴w合成了 6種金屬配合物,對(duì)其進(jìn)行了一系列表征,確定了其結(jié)構(gòu),并研究了六種金屬配合物的殺菌性能和緩釋效果。研究內(nèi)容主要包括:首先,以2:1的摩爾比將烯效唑(L1)和多效唑(L2)分別與過渡金屬鹽在室溫條件下攪拌,反應(yīng)得到了 6種金屬配合物。利用元素分析以及紅外光譜等方法得到其元素比例及官能團(tuán)等信息,用X射線單晶衍射儀確定晶體結(jié)構(gòu)。利用SHELXTL程序進(jìn)行晶體結(jié)構(gòu)解析,并分析其結(jié)構(gòu)特征,H鍵連接方式以及三維堆積方式。其次,對(duì)合成的6種金屬配合物進(jìn)行了生物活性研究。采用菌絲的生長速率法,在實(shí)驗(yàn)中測定了配合物、配體以及金屬鹽對(duì)蘋果干腐病菌,蘋果輪紋病菌,小麥赤霉病菌,葡萄黑豆病菌的抗真菌活性,并通過抑菌率及EC50值兩個(gè)指標(biāo)來評(píng)估抗菌性能。實(shí)驗(yàn)結(jié)果表明:[CoL14Cl2]的抑菌效果在烯效唑的三種金屬配合物中最顯著,尤其是對(duì)葡萄黑痘病菌(Ⅳ)的殺菌活性達(dá)到烯效唑配體的11.67倍,對(duì)其他幾種菌也有較高的殺菌活性。多效唑的三種金屬配合物中,[CuL24Br2]對(duì)蘋果輪紋病菌(Ⅱ)、小麥赤霉病菌(Ⅲ)、葡萄黑痘病菌(Ⅳ)的殺菌活性都是最好的。最后,利用高效液相色譜儀(HPLC)測定了 6種配合物的緩釋性能。首先通過對(duì)不同濃度標(biāo)準(zhǔn)烯效唑(L1)和多效唑(L2)溶液的測定,得到2種配體的HPLC標(biāo)準(zhǔn)曲線。然后測定所合成的六種配合物置于水中不同時(shí)間段緩慢釋放的配體峰面積,并且通過濃度和峰面積的關(guān)系進(jìn)一步計(jì)算其緩釋率,用所得數(shù)據(jù)繪制時(shí)間-釋放率圖,分析緩釋性能。
[Abstract]:Because of its high efficiency, broad spectrum and low toxicity, triazole fungicides have outstanding advantages in the field of agricultural sterilization.Environmental friendly new fungicides are imperative.In recent years, some researchers have found that pesticide fungicides and transition metals form metal complexes through coordination reactions, which can improve bactericidal activity and reduce toxicity and environmental pollution.On the basis of the above advantages, six metal complexes were synthesized with Uniconazole and paclobutrazole as ligands. Their structures were characterized, and the bactericidal properties and slow-release effects of the six metal complexes were studied.The main contents are as follows: firstly, six kinds of metal complexes were obtained by stirring with transition metal salts at room temperature at 2:1 molar ratio of Uniconazole L _ 1) and paclobutrazole L _ 2.The element ratio and functional group were obtained by elemental analysis and infrared spectroscopy. The crystal structure was determined by X-ray single crystal diffractometer.The crystal structure was analyzed by SHELXTL program, and the structure characteristics of H bond and three dimensional stacking were analyzed.Secondly, the bioactivity of the six metal complexes was studied.The antifungal activities of complexes, ligands and metal salts against dry rot fungus, rotavirus, scab of wheat and black bean of grape were determined by the method of mycelium growth rate.Bacteriostatic rate and EC50 value were used to evaluate the antibacterial performance.The results showed that the bacteriostatic effect of [CoL14Cl2] was the most significant in the three metal complexes of Uniconazole, especially the bactericidal activity of acidazole (鈪，

本文編號(hào)：1770991