本文選題：姜黃素　切入點：金屬離子　出處：《石河子大學》2017年碩士論文

【摘要】：隨著社會科技的發(fā)展以及人們對生物資源的深入研究,新型藥物的研究重點逐漸轉(zhuǎn)向天然產(chǎn)物的開發(fā)和利用。姜黃素是從姜科類植物中提取的一種具有廣泛藥用價值的生物成分,具有抗氧化、抗炎、抗菌、抗腫瘤等生物活性。Al~(3+)、Fe~(3+)、Cu~(2+)、Zn~(2+)是人體中重要的金屬元素,對人體的各項生理及系統(tǒng)功能及新陳代謝中起著重要的作用。由于稀土元素在生物化學中的作用,促進了稀土配合物的發(fā)展應用。同時稀土元素具有特殊的光學、電學、磁性能,可作為生產(chǎn)制造出多種功能材料的基質(zhì)和激活劑,在多領域有著良好的用途。稀土元素是親氧的元素,稀土配合物的特征配位原子是氧,它們可以與含氧光電配體的β-二酮結(jié)構(gòu)的姜黃素生成配合物。由于稀土與姜黃素生成的配合物具有優(yōu)良的發(fā)光性能和藥用作用,可作為發(fā)光材料、藥用成分等被人們重視,并進行了廣泛的研究。本實驗研究了姜黃素與金屬(Al、Fe、Cu、Zn)以及六種稀土元素(Y,Ce,Sm,Eu,,Tb,Er)的相互作用,合成了姜黃素金屬配合物。利用紫外可見光譜和熒光光譜對合成配合物的實驗條件諸如緩沖液的選擇,pH值的影響,溫度,反應時間,表面活性劑等進行了優(yōu)化。并利用其體系熒光變化形成熒光探針,進行對部分藥物和其它分子成分的定量測定和檢測。通過對姜黃素金屬配合物的熒光性質(zhì)研究,以及在引入第三配體前后其體系熒光強度的變化,本實驗發(fā)現(xiàn)了姜黃素-Cu~(2+)-異煙肼熒光體系檢測異煙肼;姜黃素-Y~(3+)-氯諾昔康熒光體系檢測氯諾昔康。通過與其它檢測方法相比,本實驗方法由于具有高準確性、高靈敏度等特點,在熒光分光光度法測定藥物成分領域具有很好的應用前景。在研究β-二酮與稀土離子作用的實驗后,對于同樣具有β-二酮結(jié)構(gòu)的氯諾昔康進行了研究,通過氯諾昔康-Eu~(3+)-溶菌酶三元配合物的合成及Eu~(3+)的熒光增強性質(zhì),建立了一種簡單、靈敏的測定氯諾昔康熒光分析方法。其結(jié)果具有高靈敏度、高準確性及良好的選擇性。這種熒光分光光度法測定氯諾昔康的方法可以很好的在醫(yī)學領域進行應用
[Abstract]:With the development of social science and technology and the deep research on biological resources, the research focus of new drugs is gradually turning to the development and utilization of natural products.Curcumin is a kind of biological component with extensive medicinal value extracted from the family Curcumae. It has biological activities of anti-oxidation, anti-inflammation, anti-bacterial and anti-tumor. It is an important metal element in human body.It plays an important role in the physiological and systemic functions and metabolism of human body.The role of rare earth elements in biochemistry promotes the development and application of rare earth complexes.At the same time, rare earth elements have special optical, electrical and magnetic properties, which can be used as the matrix and activator for the production of various functional materials, and have good applications in many fields.Rare earth elements are oxyphilic elements, and the characteristic coordination atoms of rare earth complexes are oxygen, which can form complexes with curcumin with 尾 -diketone structure of optoelectronic ligands containing oxygen.Because the complexes of rare earth with curcumin have excellent luminescent properties and medicinal functions, they can be used as luminescent materials and medicinal components, and have been widely studied.In this paper, the interaction of curcumin with the metal Alniferoferin (Cuzn) and six rare earth elements (YCe, Sm, OU, TbCU) has been studied, and the curcumin metal complexes have been synthesized.UV-Vis spectra and fluorescence spectra were used to optimize the experimental conditions such as the pH value of buffer solution, temperature, reaction time, surfactant and so on.The fluorescence probe was formed by the fluorescence change of the system, and the quantitative determination and detection of some drugs and other molecular components were carried out.The fluorescence properties of curcumin metal complexes and the changes of fluorescence intensity of the system before and after the introduction of the third ligand were studied.Determination of lornoxicam by curcumin-yangzhuan3-lornoxicam fluorescence system.Compared with other detection methods, this method has a good application prospect in the field of fluorescence spectrophotometry because of its high accuracy and high sensitivity.After studying the interaction of 尾 -diketone with rare earth ions, the chlornoxicam with 尾 -diketone structure was studied, and the fluorescence enhancement properties of lornoxicam (Eu~(3) were studied by the synthesis of chlornoxicam -Euchloride-3-lysozyme ternary complex and the fluorescence enhancement of Lornoxicam.A simple and sensitive method for the determination of lornoxicam was established.The results show high sensitivity, high accuracy and good selectivity.The method for the determination of lornoxicam by fluorescence spectrophotometry can be well applied in the field of medicine.
【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O641.4;TQ460.1

【參考文獻】

相關期刊論文 前10條

1 楊麗艷;王化泉;邵宗鴻;;缺鐵及鐵代謝異常的研究進展[J];中華醫(yī)學雜志;2016年28期

2 韓健;;食品中鋁的檢測及對人體的危害[J];食品安全導刊;2016年21期

3 周美;陳華國;周欣;龔小見;;正交實驗法優(yōu)化姜黃中姜黃素提取工藝及其抗氧化活性[J];醫(yī)藥導報;2015年10期

4 朱乾華;;不同有機堿對銪-2-羥基喹啉-4-羧酸配合物發(fā)光性能的影響[J];發(fā)光學報;2015年07期

5 歐海龍;張慶海;;姜黃素對果蠅抗氧化能力和衰老的影響[J];云南農(nóng)業(yè)大學學報(自然科學);2015年04期

6 郭官在;;補充鋅元素對高強度運動腎促紅細胞生成影響[J];科技通報;2014年12期

7 萬勝利;楊林;朱必越;鐘萌;王紅;張景R，

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