本文選題：復序橐吾　切入點：氨基酸　出處：《中華中醫(yī)藥雜志》2016年12期

【摘要】：目的:測定復序橐吾中游離和水解氨基酸的含量。方法:以異硫氰酸苯酯(PITC)為柱前衍生試劑,用反相高效液相色譜法(RP-HPLC)測定復序橐吾中氨基酸的含量。結(jié)果:21種氨基酸的檢測濃度線性范圍為0.0022-2.1000μmol/L,精密度RSD在0.38%-1.05%之間;穩(wěn)定性RSD在0.46%-1.28%之間;游離氨基酸和水解氨基酸的重復性RSD在1.20%-2.38%和0.86%-2.43%之間;游離氨基酸和水解氨基酸的加樣平均回收率分別在97.89%-101.05%和98.04%-103.03%之間且RSD均小于4%,游離和水解氨基酸的含量分別為0.2307%和8.7943%。結(jié)論:復序橐吾中含有21種氨基酸,并確定了其含量。
[Abstract]:Objective: to determine the content of free and hydrolyzed amino acids in Ligularia complex.Methods: the content of amino acids in Ligularia complex was determined by reversed-phase high performance liquid chromatography (RP-HPLC) with phenylthiocyanate (PITC) as precolumn derivatization reagent.The average recoveries of free amino acids and hydrolyzed amino acids were 97.89-101.05% and 98.04% -103.03%, respectively, and RSD were less than 43.03%. The contents of free and hydrolyzed amino acids were 0.2307% and 8.7943, respectively.Conclusion: Ligularia polyphylla contains 21 amino acids and its content is determined.
【作者單位】： 吉林農(nóng)業(yè)大學中藥材學院;吉林省食品藥品審評中心;
【基金】：國家科技支撐計劃項目(No.2011BAI03B01) 國家公益性行業(yè)科研專項(No.201303111) 科技部科技人員服務企業(yè)項目(No.2009GJB10031) 吉林省科技發(fā)展計劃項(No.20130303094,No.20140204 013YY,No.20140311050YY,No.20150307012YY)~~
【分類號】：R284.1;O657.72

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1 朱梅;長白山區(qū)復序橐吾和狹苞橐吾生物活性成分研究[D];吉林農(nóng)業(yè)大學;2013年

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