本文選題：電化學(xué)傳感器　切入點：碳納米材料　出處：《江西農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：痛風(fēng)是一種普遍存在的反復(fù)發(fā)作性炎性疾病,早在二十世紀中期我國就已經(jīng)出現(xiàn)了此類病例。研究發(fā)現(xiàn),人體內(nèi)尿酸(UA)產(chǎn)生過多是引起痛風(fēng)的直接原因,而人體血液和組織液中嘌呤含量的升高則是產(chǎn)生痛風(fēng)的根本原因,因而開發(fā)一種快速、簡便、高效的嘌呤化合物分析方法具有重要的研究意義和應(yīng)用價值。與傳統(tǒng)的檢測方法相比,電化學(xué)傳感分析由于具有靈敏性高、選擇性好、儀器簡單易操作、檢測成本低及可在線分析等特點,成為分析方法的一個重要研究方向。本論文通過結(jié)合納米粘土材料和碳納米材料的優(yōu)點,利用羧甲基纖維素鈉(CMC)良好的親水性和粘附性,制備了水可加工性的、經(jīng)濟環(huán)保的嘌呤化合物納米復(fù)合物傳感器;圍繞所構(gòu)筑傳感器的選擇性、靈敏度、穩(wěn)定性和實用性等特征展開了相應(yīng)的研究,并成功應(yīng)用于實際樣品中嘌呤化合物的檢測。具體結(jié)論如下:(1)通過選擇強吸附性的埃落石納米管(HNTs)和具有較好導(dǎo)電能力和催化能力的羧基功能化多壁碳納米管(fMWCNTs)為主體材料,并借助于具有良好粘附性、親水性的CMC溶液分散復(fù)合后,制備出具有良好電化學(xué)響應(yīng)的CMC-HNTsfMWCNTs/GCE復(fù)合修飾電極。該復(fù)合修飾電極由于具有良好的穩(wěn)定性、選擇性和靈敏度,在最佳的實驗條件下,將構(gòu)建的電化學(xué)傳感器用于UA、腺嘌呤(A)和鳥嘌呤(G)的同時電化學(xué)檢測,線性響應(yīng)范圍分別為1 130μmol·L-1、0.05 9μmol·L-1和0.3 25μmol·L-1,檢出限分別為0.5μmol·L-1、0.018μmol·L-1和0.019μmol·L-1。此外,CMC-HNTs-fMWCNTs修飾電極被成功的應(yīng)用于人體血清和生菜樣品中UA、A和G的同時檢測。(2)在CMC的水溶液中,將吸附性強、比表面積大的凹凸棒石(ATP)與導(dǎo)電能力好的氮摻雜石墨烯(NG)進行分散復(fù)合,制備出能檢測UA、黃嘌呤(XA)和次黃嘌呤(HX)的CMC-ATP-NG/GCE。該修飾電極具有良好的穩(wěn)定性、選擇性和靈敏度,對UA、XA和HX的線性范圍分別為0.5 60μmol·L-1、0.2 20μmol·L-1和0.6 55μmol·L-1,檢出限分別為0.13μmol·L-1、0.054μmol·L-1和0.39μmol·L-1。此外,CMC-ATP-NG修飾電極被成功的應(yīng)用于水果和蔬菜樣品中UA、XA和HX的同時檢測。
[Abstract]:Gout is a common recurrent inflammatory disease, as early as the middle of the twentieth century, such cases have appeared in China.The study found that too much uric acid UAA is the direct cause of gout, and the increase of purine content in human blood and tissue fluid is the root cause of gout.The efficient method of purine compound analysis has important significance and application value.Compared with the traditional detection methods, electrochemical sensing analysis has become an important research direction because of its high sensitivity, good selectivity, simple and easy to operate instruments, low detection cost and on-line analysis.In this paper, by combining the advantages of nano-clay and carbon nanomaterials, a water-processable, economical and environmentally friendly purine compound sensor was prepared by utilizing the good hydrophilicity and adhesiveness of CMC-based sodium carboxymethyl cellulose.The selectivity, sensitivity, stability and practicability of the sensors were studied and successfully applied to the detection of purine compounds in practical samples.The specific conclusions are as follows: (1) by selecting HNTs with strong adsorbability and carboxyl functionalized multiwalled carbon nanotubes (FMWCNTs) with good conductivity and catalytic ability, and with the aid of good adhesion,The CMC-HNTsfMWCNTs/GCE composite modified electrode with good electrochemical response was prepared after dispersion of hydrophilic CMC solution.Due to its good stability, selectivity and sensitivity, the electrochemical sensor was used for simultaneous electrochemical detection of UAA and guanine guanine (G) under the best experimental conditions.The linear response ranges were 1: 130 渭 mol L -1 0. 05 渭 mol L -1 and 0 30 渭 mol L -1. The detection limits were 0. 5 渭 mol L -1 0. 018 渭 mol L -1 and 0.019 渭 mol L -1, respectively.In addition, CMC-HNTs-fMWCNTs modified electrode has been successfully used in the simultaneous detection of UAA and G in human serum and lettuce samples.High specific surface area attapulgite ATP-NGN / GCEs were prepared by dispersion and compounding with nitrogen-doped graphene (NGN) with high specific surface area. CMC-ATP-NGR / GCEs were prepared for the detection of UAA, xanthine (XA) and Hypoxanthine (HX).In addition, CMC-ATP-NG modified electrode was successfully used for simultaneous detection of UAXA and HX in fruit and vegetable samples.
【學(xué)位授予單位】：江西農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.1;R446.1;TS255.7

【參考文獻】

相關(guān)期刊論文 前9條

1 王永成;陳濤;石婷;王智文;趙學(xué)明;;嘌呤核苷及其衍生物的代謝工程[J];中國生物工程雜志;2015年05期

2 ;高尿酸血癥和痛風(fēng)治療的中國專家共識[J];中華內(nèi)分泌代謝雜志;2013年11期

3 董存智;穆志英;;吖啶黃熒光光度法測定腺嘌呤[J];分析試驗室;2011年04期

4 張敏;岳寧寧;張苗;呂慶鑾;王懷友;;熒光猝滅法測定次黃嘌呤及機理研究[J];分析試驗室;2009年04期

5 金麗虹;申炳俊;翁占坤;向陽;田堅;;毛細管電動色譜法用于DNA水解產(chǎn)物堿基測定[J];長春理工大學(xué)學(xué)報(自然科學(xué)版);2008年03期

6 王滔;薛冰純;劉二保;;化學(xué)發(fā)光法測定巰嘌呤的含量[J];光譜學(xué)與光譜分析;2008年05期

7 王志賢;王峧胤;胡效亞;;化學(xué)修飾電極的制備及其藥物分析應(yīng)用的研究進展[J];化學(xué)傳感器;2007年04期

8 童萍;何聿;張?zhí)m;;毛細管電泳-柱端安培檢測法用于抗癌藥物2-氨基-6-巰基嘌呤和8-氮雜鳥嘌呤的研究[J];分析測試技術(shù)與儀器;2007年01期

9 姜旭淦,陳盛霞;毛細管電泳技術(shù)及其在生物分子研究中的應(yīng)用進展[J];江蘇大學(xué)學(xué)報(醫(yī)學(xué)版);2004年05期

，

本文編號：1713645