本文選題：TTHA衍生物　切入點(diǎn)：稀土配合物　出處：《內(nèi)蒙古大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：本文利用已有的文獻(xiàn)和方法首次合成了一系列水溶性很好的三乙四胺六乙酸(TTHA)氨基酸衍生物稀土配合物,稀土元素為鑭(La)、鈰(Ce)、釹(Nd)、釓(Gd),所用氨基酸為蘇氨酸(Thr)、纈氨酸(Val)和谷氨酸(Glu)。該系列稀土配合物通過紅外光譜、元素分析和熱分析進(jìn)行實(shí)驗(yàn)表征。TTHA氨基酸衍生物稀土配合物與大腸桿菌DNA(pBR322)特異性識別片段相連,再與pBR322進(jìn)行靶向切割。運(yùn)用瓊脂糖凝膠電泳和原子力顯微鏡(AFM)的檢測手段來探究這一系列配合物對pBR322的作用情況。本文詳細(xì)研究了 DNA的AFM樣的制作過程,此外,通過設(shè)計(jì)合成靶向探針寡聚核苷酸(ODN)并與TTHA氨基酸衍生物稀土配合物相連,從而形成對pBR322 DNA具有靶向切割作用的稀土配合物,再與pBR322 DNA進(jìn)行作用。用瓊脂糖凝膠電泳從宏觀方面討論了 TTHA氨基酸衍生物稀土配合物對pBR322 DNA的切割作用。通過原子力顯微鏡在接觸模式下對pBR322DNA成像,從微觀上進(jìn)行觀測并分析pBR322 DNA構(gòu)型構(gòu)象的變化。結(jié)果表明在37℃和pH =8.0的生理?xiàng)l件下,合成的一系列稀土配合物能夠靶向切割雙螺旋DNA。
[Abstract]:In this paper, a series of rare earth complexes of triethylenetetramine hexaacetic acid (TTHAA) amino acid derivatives were synthesized for the first time by using existing literatures and methods. The rare earth elements are lanthanum, cerium, neodymium, neodymium, gadolinium, and amino acids such as threonine, Thyr, valine, valine, and glutamate. The rare earth complexes pass through infrared spectroscopy. Elemental analysis and thermal analysis showed that the rare earth complexes of the amino acid derivatives of TTHA were linked to the specific recognition fragment of Escherichia coli DNA pBR322. Then we used agarose gel electrophoresis and atomic force microscopy (AFM) to investigate the effect of the complexes on pBR322. The preparation of AFM sample of DNA was studied in detail. By designing and synthesizing oligonucleotide oligodeoxynucleotides (ODN) targeting probe and connecting with rare earth complexes of TTHA amino acid derivatives, rare earth complexes with targeted cleavage to pBR322 DNA were formed. The effect of rare earth complexes of TTHA amino acid derivatives on pBR322DNA was discussed macroscopically by agarose gel electrophoresis. PBR322DNA was imaged by atomic force microscope in contact mode. The conformation changes of pBR322 DNA were observed and analyzed microscopically. The results showed that a series of rare earth complexes could target to cut double helix DNAs at 37 鈩，

本文編號：1644162