本文選題：銀納米粒子　切入點(diǎn)：銀離子　出處：《河北大學(xué)》2016年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：銀作為一種古老的殺菌劑,早在遠(yuǎn)古時(shí)代就被人們所認(rèn)知和使用。今天,在這個(gè)抗生素被濫用的時(shí)代,銀基殺菌劑以其特有的廣譜性和抗耐藥性而重新受到人們的青睞。銀納米粒子和銀離子是兩種常見(jiàn)且具代表性的銀基殺菌劑。近年來(lái),人們對(duì)這兩種殺菌劑在殺菌機(jī)理方面的異同產(chǎn)生了極大興趣,希望通過(guò)對(duì)這一問(wèn)題的深入研究為銀基殺菌劑的設(shè)計(jì)和應(yīng)用提供重要借鑒。在本研究中,我們首次應(yīng)用紅外光譜技術(shù)對(duì)這兩種殺菌劑的殺菌機(jī)理在分子水平上進(jìn)行了對(duì)比研究。我們選取常見(jiàn)的大腸桿菌作為模型菌株,采集其被銀納米粒子和銀離子作用前后的紅外光譜,并在多個(gè)重要的紅外光譜區(qū)域?qū)Υ竽c桿菌受到銀殺菌作用前后的結(jié)構(gòu)和組成變化進(jìn)行了深入細(xì)致的譜學(xué)分析。這些選取的光譜區(qū)域包括對(duì)蛋白質(zhì)錯(cuò)誤折疊聚集敏感的酰胺I帶振動(dòng)區(qū)(1700-1600 cm~（-1)),對(duì)DNA構(gòu)象變化敏感的磷酸根振動(dòng)區(qū)(1300-1180 cm~（-1)),對(duì)自由巰基組成變化敏感的巰基振動(dòng)區(qū)(2580-2530 cm~（-1)),對(duì)生物膜結(jié)構(gòu)變化敏感的碳?xì)滏溦駝?dòng)區(qū)(3000-2820 cm~（-1))和脂多糖糖環(huán)振動(dòng)區(qū)(1200-900 cm~（-1))。通過(guò)對(duì)比研究我們發(fā)現(xiàn),銀納米粒子和銀離子在殺菌機(jī)制上存在整體殺菌機(jī)制類(lèi)似而具體動(dòng)力學(xué)過(guò)程不同的特點(diǎn)。就二者整體殺菌機(jī)制而言,我們發(fā)現(xiàn)銀納米粒子和銀離子都通過(guò)如下四種方式和大腸桿菌發(fā)生作用,即:(1)銀和大腸桿菌內(nèi)的自由巰基發(fā)生配位絡(luò)合;(2)銀使大腸桿菌內(nèi)蛋白質(zhì)發(fā)生錯(cuò)誤折疊聚集;(3)銀導(dǎo)致大腸桿菌生物膜上的脂多糖發(fā)生缺失;(4)銀能引起大腸桿菌內(nèi)的DNA發(fā)生構(gòu)象改變。就二者和大腸桿菌發(fā)生作用時(shí)的具體動(dòng)力學(xué)過(guò)程而言,我們發(fā)現(xiàn),在銀和自由巰基配位以及引起脂多糖缺失方面,銀納米粒子和大腸桿菌的作用是一個(gè)慢動(dòng)力學(xué)過(guò)程,而銀離子和大腸桿菌的作用卻是一個(gè)快動(dòng)力學(xué)過(guò)程。對(duì)于銀納米粒子和銀離子和大腸桿菌作用時(shí)呈現(xiàn)出的這種動(dòng)力學(xué)上的差異,我們從銀納米粒子殺菌的粒子效應(yīng)的角度出發(fā),提出了一種合理的解釋。
[Abstract]:Silver as an ancient fungicide, as early as in ancient times is known and used. Today, the abuse of antibiotics in this era, silver bactericide with its unique broad-spectrum resistance and resistance to by people of all ages. The silver nanoparticles and silver ions are two common and representative silver bactericide. In recent years, people have a great interest in the two kinds of fungicides in the sterilization mechanism of the similarities and differences of hope through in-depth study on this issue provides an important reference for the design and application of silver based fungicides. In this study, we first used the sterilization mechanism of infrared spectroscopy of the two kinds of sterilization agent were investigated at the molecular level. We selected common strains of Escherichia coli as a model, the infrared spectrum before and after the acquisition of silver nanoparticles and silver ions, and in a number of important red outside The spectral region of Escherichia coli by silver structure before and after sterilization and changes in the composition of a detailed spectrum analysis. The selected spectral region including the misfolded protein aggregation sensitive amide I band vibration area (1700-1600 cm~ (-1)), the conformational change of DNA sensitive phosphate (1300-1180 cm~ (vibration area -1)), of free sulfhydryl groups composed of thiol sensitive vibration area changes (2580-2530 cm~ (-1)), the hydrocarbon chain vibration area sensitive to biofilm structure changes (3000-2820 cm~ (-1)) and lipopolysaccharide sugar ring vibration area (1200-900 cm~ (-1)). Through the comparative study we found that silver nanoparticles and silver ion sterilization mechanism and specific similar overall dynamic process of different characteristics in the sterilization mechanism. On the whole the two sterilization mechanism, we found that silver nanoparticles and silver ions through the following four ways and Escherichia coli as 鐢，

本文編號(hào)：1639938