本文選題：花椒　切入點(diǎn)：非法添加物　出處：《湖北工業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：近年來(lái),花椒安全事件已經(jīng)嚴(yán)重危及了人們的身體健康,人們?cè)絹?lái)越關(guān)注花椒中非法添加物問(wèn)題。目前,檢測(cè)花椒中非法添加物的方法很多,但大多數(shù)都是生物化學(xué)方法,這些方法的樣品制備過(guò)程十分復(fù)雜,而且必須在實(shí)驗(yàn)室使用高端設(shè)備才能進(jìn)行檢測(cè),沒(méi)有方便、快捷的檢測(cè)方法。為了解決這一問(wèn)題,本文研究了一種激光拉曼方法來(lái)檢測(cè)花椒中的非法添加物。首先,本文詳細(xì)闡述了拉曼散射的基本原理,然后設(shè)計(jì)了一個(gè)便攜式的激光拉曼光路,選用各種光學(xué)元器件完成了該光路,并驗(yàn)證了該光路的可行性。本文采用兩種方法來(lái)增強(qiáng)拉曼信號(hào),一是保證激光打在樣品的焦點(diǎn)位置,另外一個(gè)是選用合適的拉曼增強(qiáng)試劑。本文還采用了一個(gè)黑色的密閉裝置對(duì)檢測(cè)過(guò)程中出現(xiàn)的背景噪聲進(jìn)行了處理,以保證檢測(cè)的準(zhǔn)確性。其次,本文通過(guò)分析花椒中非法添加物的光譜特性,制定了相應(yīng)的檢測(cè)方案。該方案的思路是先選擇合適的溶劑,制備樣品溶液然后進(jìn)行檢測(cè)。按照該思路,該方案是通過(guò)檢測(cè)純的非法添加物溶液并分析其光譜圖,選擇合適的溶劑,然后再檢測(cè)混有這些非法添加物的花椒溶液并分析其光譜圖。本文檢測(cè)了花椒中的三種非法添加物:羅丹明B、堿性橙Ⅱ、堿性嫩黃,本文主要的檢測(cè)對(duì)象是羅丹明B。本文處理了三種濃度的羅丹明B水溶液和甲醇溶液的拉曼光譜圖,并對(duì)這些數(shù)據(jù)進(jìn)行了分析以后,選擇了對(duì)這些非法添加物檢測(cè)影響較小的溶劑:水。在這個(gè)基礎(chǔ)上,本文檢測(cè)了三種濃度的樣品溶液的拉曼光譜圖,它們分別是混有羅丹明B的花椒溶液、混有堿性橙Ⅱ的花椒溶液和混有堿性嫩黃的花椒溶液,并對(duì)這些拉曼光譜數(shù)據(jù)進(jìn)行處理與分析。最后,本文提出了一種基于多項(xiàng)式擬合的基底漂移校正算法,以解決在檢測(cè)過(guò)程中出現(xiàn)的基線漂移現(xiàn)象。該算法結(jié)合GIFTS方法和最小二乘法,模擬出了拉曼基線,將原始光譜與基線相減,從而實(shí)現(xiàn)基線的校正。本文的研究結(jié)果表明,用激光拉曼檢測(cè)方法檢測(cè)花椒中的非法添加物時(shí),制樣簡(jiǎn)單、檢測(cè)速度快、檢測(cè)結(jié)果比較理想,為激光拉曼檢測(cè)方法在調(diào)味品中的進(jìn)一步應(yīng)用提供了一些實(shí)際的參考依據(jù)。
[Abstract]:In recent years, pepper safety incidents have seriously endangered people's health, people are increasingly concerned about the problem of illegal additives in pepper. At present, there are many methods for detecting illegal additives in Chinese pepper, but most of them are biochemical methods. The process of sample preparation for these methods is very complex, and it is necessary to use high-end equipment in the laboratory in order to carry out the test. There is no convenient and quick test method. In order to solve this problem, In this paper, a laser Raman method is studied to detect illegal additives in prickly ash. Firstly, the basic principle of Raman scattering is described in detail, and then a portable laser Raman light path is designed. All kinds of optical components are used to complete the optical circuit, and the feasibility of the optical path is verified. In this paper, two methods are used to enhance the Raman signal, one is to ensure that the laser is in the focal position of the sample. The other is to select the appropriate Raman intensifier. In this paper, a black sealing device is used to deal with the background noise in the detection process to ensure the accuracy of the detection. In this paper, by analyzing the spectral characteristics of illegal additives in Zanthoxylum bungeanum, a corresponding detection scheme has been established. The idea of the scheme is to first select the appropriate solvent, prepare the sample solution and then detect it. The scheme is to select the appropriate solvent by detecting the pure illegal additive solution and analyzing its spectrogram. Then we detected the pepper solution mixed with these illegal additives and analyzed their spectrogram. In this paper, we detected three kinds of illegal additives in pepper: Rhodamine B, alkaline orange 鈪，

本文編號(hào)：1637808