本文選題：氫醌　切入點(diǎn)：苯酚　出處：《藥物分析雜志》2017年03期 　論文類(lèi)型：期刊論文

【摘要】：目的:對(duì)高效液相色譜法測(cè)定美白類(lèi)化妝品中氫醌、苯酚的色譜條件進(jìn)行優(yōu)化。方法:以系統(tǒng)適用性為指標(biāo),比較了色譜柱類(lèi)型、緩沖鹽種類(lèi)及流動(dòng)相p H對(duì)氫醌、苯酚和化妝品樣品分離效果的影響。對(duì)優(yōu)化后色譜條件進(jìn)行了方法學(xué)考察,并在此條件下對(duì)抽檢的化妝品樣品進(jìn)行測(cè)定。結(jié)果:最優(yōu)色譜條件:采用C_(18)(4.6 mm×250 mm,5μm)色譜柱,以10 mmol·L~(-1)磷酸二氫鉀水溶液(磷酸調(diào)p H 3.5)-甲醇為流動(dòng)相進(jìn)行梯度洗脫,流速1mL·min~(-1),柱溫25℃,檢測(cè)波長(zhǎng)280 nm,進(jìn)樣量5μL。在最優(yōu)色譜條件下,氫醌質(zhì)量濃度在0.4~40μg·mL~(-1)范圍內(nèi),苯酚質(zhì)量濃度在0.7~70μg·mL~(-1)范圍內(nèi)線(xiàn)性關(guān)系良好,相關(guān)系數(shù)分別為0.999 8和0.999 9,氫醌、苯酚檢測(cè)限分別為0.000 5和0.001μg,定量限分別為0.002和0.003 5μg,精密度試驗(yàn)的RSD分別為0.4%~1.0%和0.3%~1.8%,回收率分別為98.4%~99.5%和100.4%~102.2%。對(duì)市售的美白祛斑類(lèi)化妝品200批進(jìn)行測(cè)定,在本文的樣品處理方法下氫醌和苯酚的檢出限分別為1和2μg·g~(-1),定量限分別為4和7μg·g~(-1),雜質(zhì)峰和目標(biāo)峰能夠?qū)崿F(xiàn)基線(xiàn)分離、峰形對(duì)稱(chēng)。結(jié)論:本法測(cè)定美白祛斑類(lèi)化妝品中氫醌、苯酚含量的專(zhuān)屬性好,可以為化妝品檢驗(yàn)標(biāo)準(zhǔn)的改進(jìn)提供參考。
[Abstract]:Objective: to optimize the chromatographic conditions for the determination of hydroquinone and phenol in whitening cosmetics by high performance liquid chromatography. The effect of phenol and cosmetics on the separation of samples was investigated. The optimized chromatographic conditions were investigated, and the samples of cosmetics were determined under these conditions. Results: the optimum chromatographic conditions were as follows: Che 184.6mm 脳 250mm 脳 5 渭 m) column. 10 mmol 路L ~ (-1) potassium dihydrogen phosphate solution was used as mobile phase, flow rate was 1 mL 路min ~ (-1) ~ (-1), column temperature was 25 鈩，

本文編號(hào)：1629848