本文選題：電化學(xué)傳感器　切入點：比率　出處：《青島大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：生物體內(nèi)一些重要分子,如:鹽酸阿霉素(DOX)等抗腫瘤藥物,多巴胺(DA)等疾病標(biāo)志物的檢測對人體健康以及臨床生化分析意義重大。熒光法,比色法,電泳法,色譜法等常見的分析方法面臨耗費(fèi)高,樣品制備復(fù)雜等問題。電化學(xué)傳感器由于靈敏度高,響應(yīng)快,簡便,成本低,體內(nèi)檢測等特點,受到廣泛關(guān)注。傳統(tǒng)的單信號電化學(xué)傳感器對實驗室檢測條件的嚴(yán)重依賴的技術(shù)缺陷,限制了它的發(fā)展應(yīng)用。比率電化學(xué)傳感器引入了內(nèi)在校準(zhǔn)機(jī)制,可有效降低傳感體系內(nèi)的背景電信號,進(jìn)而提高電化學(xué)傳感的準(zhǔn)確性,重現(xiàn)性和靈敏性�；诖�,本課題基于電化學(xué)比率技術(shù)構(gòu)建了新型的比率電化學(xué)生物傳感器對DOX、DA進(jìn)行檢測,具體研究如下:一、設(shè)計了一種直接基于裸玻碳電極檢測常規(guī)電解液中的目標(biāo)物的新型雙比率電化學(xué)DOX傳感器。本研究選取具有不同氧化峰電位的三種物質(zhì),提供內(nèi)部參比信號的二茂鐵(Fc,0.15 V)和亞甲基藍(lán)(MB,-0.26 V),以及作為分析物的DOX(-0.62 V)后,制備出三者的混合電解液體系。通過直接使用裸玻碳電極作為雙信號傳感平臺,測量含有固定濃度Fc、MB和不同濃度DOX的三種組分的各電解質(zhì)溶液的方波伏安曲線。實驗結(jié)果表明,該傳感體系具有類似甚至優(yōu)于其他修飾電極體系的寬線性范圍和低檢測限。此外,該雙比率電化學(xué)傳感器具有良好的選擇性和穩(wěn)定性,可用于實際血清和唾液樣品中DOX的檢測。該雙比率電化學(xué)傳感器簡易、新穎,且具備一定的普遍性,可發(fā)展出一系列的新型雙比率電化學(xué)傳感體系。二、構(gòu)建了一種基于金納米離子/電化學(xué)還原石墨烯-耐爾藍(lán)(AuNPs/ERGO-NB)納米復(fù)合材料修飾玻碳電極的新型免標(biāo)記比率電化學(xué)DA適體傳感器。基于AuNPs/ERGO-NB納米復(fù)合材料優(yōu)異的導(dǎo)電性,適體對分析物的特異性,以雙信號電流比值(IDA/INB)作為信號輸出,該比率電化學(xué)適體傳感器表現(xiàn)出長時間穩(wěn)定性和優(yōu)異的重現(xiàn)性,可以對DA實現(xiàn)高靈敏性,高選擇性,和準(zhǔn)確性地檢測。此外,該傳感器具備實際樣品檢測能力,可應(yīng)用于實際血清樣品中的DA的分析。更重要的是,該分析方法可以通過結(jié)合特定受體和靶目標(biāo)物,容易地擴(kuò)展到用于檢測其它電活性物質(zhì)的新型電化學(xué)傳感器的設(shè)計。
[Abstract]:The detection of some important molecules, such as doxorubicin hydrochloride and dopamine (DAA), is of great significance to human health and clinical biochemical analysis. Common analytical methods, such as chromatography, face high cost and complex sample preparation. Electrochemical sensors are characterized by high sensitivity, fast response, simplicity, low cost and in vivo detection. The traditional single signal electrochemical sensor, which is heavily dependent on the laboratory detection conditions, has limited its development and application. The ratio electrochemical sensor has introduced the inherent calibration mechanism. It can effectively reduce the background signal in the sensing system and improve the accuracy, reproducibility and sensitivity of the electrochemical sensor. Based on the electrochemical ratio technology, a novel ratio electrochemical biosensor was constructed to detect DOXDA. The main results are as follows: 1. A novel dual ratio electrochemical DOX sensor based on bare glassy carbon electrode was designed to detect the target in conventional electrolyte. In this study, three kinds of substances with different oxidation peak potentials were selected. A mixture electrolyte system of ferrocene (Fc0. 15 V), methylene blue (MB-0.26 V) and DOX(-0.62 V (as an analytical material) was prepared by direct use of bare glassy carbon electrode as a dual signal sensing platform. The square wave voltammetry curves of three electrolyte solutions with fixed concentrations of Fcmb and different concentrations of DOX were measured. The sensor system has a wide linear range and a low detection limit similar to or even superior to that of other modified electrode systems. In addition, the dual ratio electrochemical sensor has good selectivity and stability. It can be used for the detection of DOX in serum and saliva samples. The dual ratio electrochemical sensor is simple, novel and universal, and a series of novel dual ratio electrochemical sensing systems can be developed. A novel labeling free ratio electrochemical DA aptamer sensor based on au nano ion / electrochemical reduction graphene / Nel blue (AuNPs / ERGO-NB) nanocomposite modified glassy carbon electrode was constructed. Based on the excellent conductivity of AuNPs/ERGO-NB nanocomposites, a novel electrochemical aptamer sensor was developed. The specificity of the aptamer to the analyte. The ratio of double signal current to IDA / INB is used as the signal output. The electrochemical aptamer sensor has a long time stability and excellent reproducibility, which can achieve high sensitivity and selectivity to DA. In addition, the sensor has the ability to detect actual samples and can be applied to the analysis of DA in actual serum samples. Easy to extend to the design of new electrochemical sensors for the detection of other electrically active substances.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TP212;O657.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 祝敬妥;;電化學(xué)傳感器在應(yīng)急救援中應(yīng)用[J];廣州化工;2011年03期

2 金利通,宋豐斌,柏竹平,方禹之;味覺電化學(xué)傳感器的研究——Ⅰ.甜、酸、苦、咸物質(zhì)對模擬生物膜電位振動頻率的影響[J];分析化學(xué);1993年11期

3 金利通，，毛煜，劉彤;味覺電化學(xué)傳感器的研究──黃連素對模擬生物膜的響應(yīng)[J];分析科學(xué)學(xué)報;1994年02期

4 李星瑋,李曉宣,居明;導(dǎo)電聚苯胺在化學(xué)及電化學(xué)傳感器中的應(yīng)用[J];化工新型材料;2000年09期

5 王春,何錫文;超分子在質(zhì)量敏感壓電化學(xué)傳感器中的應(yīng)用[J];分析測試學(xué)報;2001年01期

6 陸曉軍,鞠q

本文編號：1629665