本文選題：木質(zhì)素　切入點(diǎn)：微區(qū)分布　出處：《光譜學(xué)與光譜分析》2017年10期 　論文類型：期刊論文

【摘要】：整合共聚焦顯微熒光和拉曼光譜成像技術(shù)系統(tǒng)研究了黃藤藤莖組織中不同類型細(xì)胞以及同一細(xì)胞不同形態(tài)區(qū)域的木質(zhì)素區(qū)域化學(xué)特點(diǎn)。共聚焦熒光成像表明黃藤藤莖組織中木質(zhì)素主要匯聚于初生木質(zhì)部導(dǎo)管、次生木質(zhì)部導(dǎo)管、維管束間的薄壁組織細(xì)胞以及纖維細(xì)胞角隅區(qū)�；跓晒夤庾V差異的光譜成像線性拆分結(jié)果顯示纖維細(xì)胞次生壁由寬、窄層交替的同心層狀結(jié)構(gòu)組成,且窄層具有更高的木質(zhì)化程度。比較黃藤、毛竹、芒草、毛白楊和虎皮松拉曼光譜發(fā)現(xiàn)黃藤材細(xì)胞壁拉曼光譜與闊葉木毛白楊類似,證實(shí)了黃藤材的化學(xué)組成更加趨近于闊葉木毛白楊。對拉曼光譜中木質(zhì)素特征峰成像進(jìn)一步揭示出纖維細(xì)胞中木質(zhì)素不均一的分布規(guī)律:其中細(xì)胞角隅胞間層和復(fù)合胞間層的拉曼信號強(qiáng)度最高,表明較高的木質(zhì)化程度,其次是次生壁中的窄層,而次生壁寬層中拉曼特征峰強(qiáng)度最低,這一分布規(guī)律與竹材纖維細(xì)胞中木質(zhì)素分布規(guī)律類似。寬、窄層中木質(zhì)素不僅存在濃度上的差異,而且木質(zhì)素基本結(jié)構(gòu)單元的比例亦不同。采取光譜去卷積的方法排除了碳水化合物的影響,發(fā)現(xiàn)窄層中愈創(chuàng)木基(G型)木質(zhì)素與紫丁香基木質(zhì)素(S型)比例為0.19,而在寬層中這一比值為0.14,這一結(jié)果亦解釋了寬、窄層熒光光譜間的差異。該研究結(jié)果對探索黃藤細(xì)胞壁生物合成及力學(xué)響應(yīng)機(jī)制研究具有重要理論指導(dǎo)意義。
[Abstract]:The chemical characteristics of lignin region in different types of cells in stem tissue and different morphological regions of the same cell were studied by integrating confocal fluorescence microscopy and Raman spectroscopy. Lignin in the stem tissue of rattan mainly converges in the primary xylem catheter. Secondary xylem ducts, parenchyma cells between vascular bundles and filamentous corner regions. The results of spectral imaging based on fluorescence spectrum difference showed that the secondary wall of fibroblasts was composed of concentric lamellar structures with wide and narrow layers alternately. The narrow layer has a higher degree of lignification. Compared with the raman spectra of Rattan, Phyllostachys pubescens, mango, Populus tomentosa and Poplar, it was found that the cell wall Raman spectra of the wood were similar to those of broadleaved Populus tomentosa. It is confirmed that the chemical composition of the wood is closer to that of hardwood Populus tomentosa. The distribution of lignin heterogeneity in fibroblasts is further revealed by the characteristic peak imaging of lignin in Raman spectroscopy: the intercellular layer of the cell horn and the intercellular layer of the cell. The Raman signal intensity of the composite intercellular layer was the highest. The results showed that the intensity of Raman characteristic peak in the broad layer of secondary wall was the lowest, which was similar to that of lignin distribution in bamboo fiber cells, and the distribution of lignin in secondary wall was similar to that of lignin in bamboo fiber cells, the second was narrow layer in secondary wall, and the intensity of Raman characteristic peak in broad layer of secondary wall was the lowest. The concentration of lignin in narrow layer is different, and the proportion of lignin basic structure unit is also different. The effect of carbohydrate is excluded by spectral deconvolution. It is found that the ratio of guaiacol G type) lignin to syringe lignin S type in narrow layer is 0.19, and the ratio in wide layer is 0.14. This result also explains the width. The results of this study have important theoretical significance for exploring the biosynthesis of cell wall and the mechanism of mechanical response.
【作者單位】： 國際竹藤中心竹藤科學(xué)與技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國際竹藤中心基本科研業(yè)務(wù)費(fèi)項(xiàng)目(1632015002) 國家自然科學(xué)基金項(xiàng)目(31500497)資助
【分類號】：O657.3;Q944.6

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本文編號：1625826