本文選題：高光譜　切入點(diǎn)：黃瓜細(xì)菌性角斑病　出處：《光譜學(xué)與光譜分析》2017年06期 　論文類型：期刊論文

【摘要】：應(yīng)用可見/近紅外高光譜對細(xì)菌性角斑病早期脅迫下的黃瓜葉片中所含過氧化物酶(peroxidase,POD)活性進(jìn)行檢測。在380~1 030nm光譜范圍獲取120個(gè)樣本(健康,病害輕微感染1級和2級)的光譜曲線,并使用分光光度計(jì)法測量感染病害樣本中的過氧化物酶活性值。采用單因素方差分析(analysis of variance,ANOVA)對三種不同程度早期病害脅迫下過氧化物酶活性值進(jìn)行統(tǒng)計(jì)分析,結(jié)果表明不同程度病害脅迫下黃瓜葉片中的過氧化物活性存在顯著性差異(p=0.05)。采用SPXY方法將樣本分為建模集(80個(gè)樣本)與預(yù)測集(40個(gè)樣本)。采用random frog(RF)和回歸系數(shù)法(regression coefficient,RC)方法提取特征波段,并建立過氧化物酶活性值的偏最小二乘回歸(partial least square regression,PLSR)預(yù)測模型。最終得到RF-PLSR具有最佳的預(yù)測效果,預(yù)測集相關(guān)系數(shù)為0.816,預(yù)測均方根誤差為11.235。研究結(jié)果表明高光譜結(jié)合化學(xué)計(jì)量學(xué)方法可以實(shí)現(xiàn)細(xì)菌性角斑病早期脅迫下黃瓜葉片中過氧化物酶活性的測定,為植物病害的早期無損診斷提供參考。
[Abstract]:The activity of peroxidase peroxidase (POD) in cucumber leaves under early stress of bacterial keratoplakia was detected by visible / near infrared hyperspectral method. 120 samples (healthy) were obtained in the spectrum range of 380 ~ 1030nm. The spectral curves of the disease with slight infection of grades 1 and 2), The peroxidase activity in infected disease samples was measured by spectrophotometer, and the peroxidase activity was analyzed by single factor analysis of variance (ANOVA) under three different degrees of early disease stress. The results showed that there was significant difference in peroxide activity in cucumber leaves under different degree of disease stress. SPXY method was used to divide the samples into modeling set (80 samples) and prediction set (40 samples). Random frogfr and regression coefficient method were used. The characteristic bands were extracted by regression regression method. A partial least square regression model for predicting peroxidase activity was established. Finally, the best prediction effect of RF-PLSR was obtained. The correlation coefficient of prediction set was 0.816, and the root mean square error of prediction was 11.235. The results showed that hyperspectral combined with chemometrics could be used to determine the activity of peroxidase in cucumber leaves under early bacterial corner spot stress. It provides reference for early nondestructive diagnosis of plant diseases.
【作者單位】： 浙江大學(xué)農(nóng)業(yè)與生物技術(shù)學(xué)院生物技術(shù)研究所;浙江大學(xué)生物系統(tǒng)工程與食品科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(31471417) 高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金項(xiàng)目(20130101110104)資助
【分類號(hào)】：O657.3;S436.421

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